DOT1L1抗体 (E-5) 推荐用于 mouse, rat 和 human 来源的DOT1L1 WB, IP, IF 和 ELISA检测

DOT1L1 抗体 (E-5): sc-374317

DOT1L1抗体 (E-5) is rated 3.5 out of 5 by 4.
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说明书

    • DOT1L1 抗体 E-5 是小鼠单克隆 IgG1 (kappa轻链),DOT1L1抗体,规格为200 µg/ml
    • 免疫原氨基酸序列1-300位于human物种的DOT1L1的N-terminus
    • DOT1L1 抗体 (E-5) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 DOT1L1
    • 抗-DOT1L1 抗体 (E-5) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 可用于ChIP的TransCruz试剂(sc-374317 X, 200 µg/0.1 ml)
    • 关于如何获取DOT1L1 (E-5): sc-374317免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 DOT1L1 Antibody (E-5) for WB 的首选应用二抗。这些试剂现在以套装形式提供DOT1L1 Antibody (E-5)。 (见下方订购信息).
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Hi I was looking to purchase an antibody for DOT1L, for use in immunoblotting and immunoprecipation. I saw the following promotion and would be interested to hear more. "Contact us to receive a FREE 10 µg sample of DOT1L1 (E-5): sc-374317".

Asked by: Janith
Thank you for your question. Please contact our Technical Service Department and we would be happy to help answer any questions you have about our current antibody sample promotion. https://www.scbt.com/scbt/resources/technical-service
Answered by: Technical Support
Date published: 2017-09-13

Are there any issues with using sc-374317: DOT1L1 (E-5) mouse monoclonal primary antibody on mouse samples?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-374317 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 3 out of 5 by from A fair antibody Western blotting with this antibody in mouse testicular tissues did not detect any bands around molecular weight of 150 to 190 kDa.
Date published: 2017-08-25
Rated 5 out of 5 by from Works great Excellent antibody! One single (and strong) band at the right molecular weight.
Date published: 2017-07-21
Rated 1 out of 5 by from Not good for IP It cannot pull down itself in co-IP experiment using human cell nuclear extract.
Date published: 2017-06-24
Rated 5 out of 5 by from Good Western blot data of DOT1L1 expression in non Good Western blot data of DOT1L1 expression in non-transfected and mouse DOT1L1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-05-30
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