DNA pol delta cat抗体 (H-8) 推荐用于 mouse, rat 和 human 来源的DNA pol δ cat WB, IP, IF 和 ELISA检测

DNA pol δ cat 抗体 (H-8): sc-374025

DNA pol δ cat抗体 (H-8) is rated 5.0 out of 5 by 4.
  • y_2021, m_1, d_27, h_17
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.13
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_zh_CN, sid_374025, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 108ms
  • REVIEWS, PRODUCT
5
4
4
0
3
0
2
0
1
0

说明书

    • DNA pol δ cat抗体(H-8)是小鼠单克隆IgG2a (lambda light chain),规格为200 µg/ml
    • 特异性抗原位于human物种的DNA pol δ cat的C-terminus附近的氨基酸1079-1116之间
    • 抗-DNA pol delta cat 抗体 (H-8) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 DNA pol δ cat; 也和以下物种反应,包括: 和 equine, canine and porcine
    • 抗-DNA pol delta cat 抗体 (H-8) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取DNA pol δ cat (H-8): sc-374025免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred detection reagent for DNA pol δ cat Antibody (H-8) for WB applications. This reagent is now offered in a bundle with DNA pol δ cat Antibody (H-8) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.

I used this antibody for WB in DN3A4(mouse NKT cell line) and Jurkat (human T cell line) whole cell lysates but there was no band. Beta-actin band was confirmed. Which cell line could be a positive control? Is there a possibility of antibody problem?

Asked by: wmtmaksm
Thank you for your question. We recommend using HeLa, HL-60, MCF7 or MOLT-4 whole cell lysates as positive controls with this antibodies. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2020-07-08

What application is the blocking peptide sc-374025 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
  • y_2021, m_1, d_27, h_17CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.13
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_374025, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 132ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from GREAT Great. This antibody is very good for IF staining.
Date published: 2017-08-17
Rated 5 out of 5 by from Works well for WB with in NIH/3T3 nuclear extract Works well for WB with in NIH/3T3 nuclear extract. -SCBT QC
Date published: 2015-05-01
Rated 5 out of 5 by from Clean blot with single strong band at expected Clean blot with single strong band at expected size using in HeLa nuclear extract. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Produced superb immunofluorescence nuclear Produced superb immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2013-03-01
  • y_2021, m_1, d_27, h_17
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.13
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_zh_CN, sid_374025, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 6ms
  • REVIEWS, PRODUCT

圣克鲁斯生物技术在生物医药研究市场的产品开发方面处于世界领先地位。欢迎 致电我们的免费电话(021) 800-820-8626。 版权 ©, 2007-2021圣克鲁斯生物技术。版权所有。“Santa Cruz Biotechnology”,圣克鲁斯生物技 术的 商标,“Santa Cruz Animal Health”,“San Juan Ranch”,“Supplement of Champions”,San Juan Ranch的商标,“Ultracruz”,“Chemcruz”,“Immunocruz”,“Exactacruz”和“EZ Touch”都是圣克鲁斯生物技术的注册商标。所有的商标都归其各自拥有者所有。