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DNA pol δ cat Antibody (H-8) - Western Blotting - Image 362812
DNA pol δ cat 抗体 (H-8): sc-374025. 对 HeLa (A), HL-60 (B), MCF7 (C) 和 MOLT-4 (D) 全细胞裂解液中 DNA pol δ cat 表达的 Western 印迹分析.
DNA pol δ cat 抗体 (H-8): sc-374025. 对 HeLa (A), HL-60 (B), MCF7 (C) 和 MOLT-4 (D) 全细胞裂解液中 DNA pol δ cat 表达的 Western 印迹分析.

DNA pol δ cat 抗体 (H-8): sc-374025

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寫評論提問

说明书
  • DNA pol δ cat抗体(H-8)是小鼠单克隆IgG2a λ, 在1篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的DNA pol δ cat的C-terminus附近的氨基酸1079-1116之间
  • 抗-DNA pol delta cat 抗体 (H-8) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 DNA pol δ cat; 也和以下物种反应，包括： 和 equine, canine and porcine
  • 抗DNA pol delta cat抗体（H-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DNA pol δ cat (H-8): sc-374025的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2a BP-HRP">m-IgG2a BP-HRP和m-IgGλ BP-HRP是DNA pol δ cat Antibody (H-8) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与DNA pol δ cat Antibody (H-8) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外DNA Polymerase抗体，包括DNA pol α, DNA pol β, DNA pol δ 2, DNA pol δ cat, DNA pol ε, DNA pol ε A, DNA pol ε B, DNA pol γ, DNA pol ι, DNA pol λ和DNA pol μ

DNA pol δ cat抗体（H-8）是一种IgG2a λ小鼠单克隆DNA pol δ cat抗体，可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的DNA pol δ cat。DNA pol δ cat抗体（H-8）可作为非偶联的抗DNA pol δ cat抗体形式，以及多种偶联形式的抗DNA pol δ cat抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。DNA复制、重组和修复，所有这些对于基因组稳定性都是必需的，需要外切核酸酶的存在。在DNA复制中，这些酶参与冈崎片段的加工，而在DNA修复中，它们的功能是切除受损的DNA片段并纠正重组错配。参与这些过程的外切核酸酶包括DNA聚合酶，包括DNA pol δ和ε。DNA pol δ由两个亚基组成，p125直接与滑动DNA夹蛋白PCNA相互作用，p50。DNA pol δ可以被细胞周期蛋白调节。DNA pol ε是一种多亚基酶，其催化亚基由POL2基因编码。由DNA pol δ和ε在引导链和滞后链上催化的确切反应尚未阐明。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DNA pol δ cat 抗体 (H-8) 参考文献:

  1. 外源性 MSH6 和 POLD1 的表达对具有突变表型的人类结肠癌细胞系 DLD-1 中 HPRT 基因座突变率的影响。  |  Yabuta, T., et al. 2004. Int J Oncol. 24: 697-702. PMID: 14767555
  2. 检测结直肠癌患者 POLE 和 POLD1 基因的等位基因变异。  |  LA, P., et al. 2017. Balkan J Med Genet. 20: 83-88. PMID: 29876237
  3. 早发结直肠癌和多发性结直肠腺瘤犹太群体中的 POLD1 和 POLE 基因突变。  |  Rosner, G., et al. 2018. Dis Colon Rectum. 61: 1073-1079. PMID: 30086056
  4. 多发性结直肠息肉患者中 POLD1 和 POLE 外切酶结构变异的频率较低。  |  Elsayed, FA., et al. 2019. Mol Genet Genomic Med. 7: e00603. PMID: 30827058
  5. POLD1 缺陷揭示了 POLD1 在 DNA 修复以及 T 细胞和 B 细胞发育中的作用。  |  Nichols-Vinueza, DX., et al. 2021. J Clin Immunol. 41: 270-273. PMID: 33140240
  6. POLD1 p.ser605del 变异的功能分析：MDPL 综合征的衰老表型与 DNA 修复能力受损有关。  |  Murdocca, M., et al. 2021. Aging (Albany NY). 13: 4926-4945. PMID: 33618333
  7. 白藜芦醇通过降低 POLD1 表达促进三阴性乳腺癌细胞凋亡  |  Liang, ZJ., et al. 2021. Front Oncol. 11: 569295. PMID: 33747905
  8. 微卫星不稳定型、微卫星稳定型或 POLE/POLD1 突变型结肠癌中的肿瘤浸润淋巴细胞、肿瘤突变负荷和遗传变异。  |  Keshinro, A., et al. 2021. JCO Precis Oncol. 5: PMID: 34250404
  9. 研究 CENPF 和 FOXM1 的功能协同发现 POLD1 是肝细胞癌的下游靶点  |  Ho, DW., et al. 2022. Front Med (Lausanne). 9: 860395. PMID: 35865168
  10. 人类 POLD1 基因。上游激活序列的鉴定、Sp1 和 Sp3 的激活以及细胞周期调控。  |  Zhao, L. and Chang, LS. 1997. J Biol Chem. 272: 4869-82. PMID: 9030545

订购信息

产品名称产品编号规格价格数量收藏夹

DNA pol δ cat 抗体 (H-8)

sc-374025
200 µg/ml
$316.00

DNA pol δ cat (H-8): m-IgGλ BP-HRP 套装

sc-522594
200 µg Ab, 40 µg BP
$354.00

DNA pol δ cat (H-8): m-IgG2a BP-HRP 套装

sc-546235
200 µg Ab; 10 µg BP
$354.00

DNA pol δ cat 抗体 (H-8) AC

sc-374025 AC
500 µg/ml, 25% agarose
$416.00

DNA pol δ cat 抗体 (H-8) HRP

sc-374025 HRP
200 µg/ml
$316.00

DNA pol δ cat 抗体 (H-8) FITC

sc-374025 FITC
200 µg/ml
$330.00

DNA pol δ cat 抗体 (H-8) PE

sc-374025 PE
200 µg/ml
$343.00

DNA pol δ cat 抗体 (H-8) Alexa Fluor® 488

sc-374025 AF488
200 µg/ml
$357.00

DNA pol δ cat 抗体 (H-8) Alexa Fluor® 546

sc-374025 AF546
200 µg/ml
$357.00

DNA pol δ cat 抗体 (H-8) Alexa Fluor® 594

sc-374025 AF594
200 µg/ml
$357.00

DNA pol δ cat 抗体 (H-8) Alexa Fluor® 647

sc-374025 AF647
200 µg/ml
$357.00

DNA pol δ cat 抗体 (H-8) Alexa Fluor® 680

sc-374025 AF680
200 µg/ml
$357.00

DNA pol δ cat 抗体 (H-8) Alexa Fluor® 790

sc-374025 AF790
200 µg/ml
$357.00

DNA pol δ cat (H-8) 中和勝肽

sc-374025 P
100 µg/0.5 ml
$68.00

I used this antibody for WB in DN3A4(mouse NKT cell line) and Jurkat (human T cell line) whole cell lysates but there was no band. Beta-actin band was confirmed. Which cell line could be a positive control? Is there a possibility of antibody problem?

Asked by: wmtmaksm
Thank you for your question. We recommend using HeLa, HL-60, MCF7 or MOLT-4 whole cell lysates as positive controls with this antibodies. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2020-07-08

What application is the blocking peptide sc-374025 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from good signal but not sure about he sizeGot a sample of this antibody to try, it worked well on both soluble lysates and chromatin (1:500 for western blot), but the band I see is juts below 150kDa, so I would say 140? Definitely not 125 as expected. I am a little concerned, but I don't have any siRNA to test it. Has this antibody been tested with siRNA?
Date published: 2021-07-20
Rated 4 out of 5 by from Right protein at the right size.Right protein, correct kDa; 1:200, could be a bit more potent.
Date published: 2021-06-13
Rated 5 out of 5 by from GREATGreat. This antibody is very good for IF staining.
Date published: 2017-08-17
Rated 5 out of 5 by from Works well for WB with in NIH/3T3 nuclear extractWorks well for WB with in NIH/3T3 nuclear extract. -SCBT QC
Date published: 2015-05-01
Rated 5 out of 5 by from Clean blot with single strong band at expectedClean blot with single strong band at expected size using in HeLa nuclear extract. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Produced superb immunofluorescence nuclearProduced superb immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2013-03-01
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DNA pol δ cat 抗体 (H-8) is rated 4.7 out of 5 by 6.
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