Date published: 2025-9-6
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DNA pol α Antibody (G-5) - Western Blotting - Image 371548
DNA pol α 抗体 (G-5): sc-365327. K-562 (A), HEL 92. 1. 7 (B) 和 Hep G2 (C) 全细胞裂解液中 DNA pol α 表达的 Western 印迹分析.
DNA pol α 抗体 (G-5): sc-365327. K-562 (A), HEL 92. 1. 7 (B) 和 Hep G2 (C) 全细胞裂解液中 DNA pol α 表达的 Western 印迹分析.

DNA pol α 抗体 (G-5): sc-365327

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寫評論提問

说明书
  • DNA pol α抗体(G-5)是小鼠单克隆IgA (kappa轻链)，规格为200 µg/ml
  • 特异性抗原位于human物种的DNA pol α的N-terminus附近的氨基酸3-31之间
  • 推荐用于 mouse, rat 和 human 来源的DNA pol α WB, IP, IF 和 ELISA检测
  • m-IgGκ BP-HRP是DNA pol α Antibody (G-5) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与DNA pol α Antibody (G-5) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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    描述
    基因信息
    说明书与实验方案
    研究信息

    関連項目

    额外DNA Polymerase抗体，包括DNA pol α, DNA pol β, DNA pol δ 2, DNA pol δ cat, DNA pol ε, DNA pol ε A, DNA pol ε B, DNA pol γ, DNA pol ι, DNA pol λ和DNA pol μ

    DNA复制、重组和修复，所有这些对基因组稳定性都是必要的，需要核酸外切酶的存在。在DNA复制中，这些酶参与冈崎片段的加工，而在DNA修复中，它们的功能是切除受损的DNA片段并纠正重组错配。这些核酸外切酶包括DNA聚合酶家族。DNA pol α、β、δ和e参与DNA复制和修复。DNA pol δ和DNA pol e是多亚基酶，DNA pol δ由两个亚基组成：p125，与滑动DNA夹蛋白PCNA相互作用；和p50。核编码的DNA pol γ是线粒体DNA复制所需的唯一DNA聚合酶。DNA pol ζ在各种组织中普遍表达，并介导损伤诱导突变的细胞机制。DNA pol θ是一种DNA聚合酶-解旋酶，结合ATP并参与链间交联的修复。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    DNA pol α 抗体 (G-5) 参考文献:

    1. 异常的DNA聚合酶α因缺陷性导入和降解而被排除在细胞核之外。  |  Eichinger, CS., et al. 2009. J Biol Chem. 284: 30604-14. PMID: 19726690
    2. 通过遗传选择确定有效启动复合物形成所需的DNA聚合酶α亚基残基和相互作用。  |  Lindow, JC., et al. 2015. J Biol Chem. 290: 16851-60. PMID: 25987558
    3. 人类DNA聚合酶α的活性和保真度取决于引物结构。  |  Baranovskiy, AG., et al. 2018. J Biol Chem. 293: 6824-6843. PMID: 29555682
    4. DNA Polymerase alpha 对乙型肝炎病毒共价闭合环状 DNA 的胞内扩增至关重要。  |  Tang, L., et al. 2019. PLoS Pathog. 15: e1007742. PMID: 31026293
    5. DNA聚合酶α与H3-H4相互作用，促进母体组蛋白向滞后链的转运。  |  Li, Z., et al. 2020. Sci Adv. 6: eabb5820. PMID: 32923642
    6. DNA聚合酶α突变可恢复WEE1KO对HU的敏感性。  |  Eekhout, T., et al. 2021. Int J Mol Sci. 22: PMID: 34502313
    7. 在压力下，检查点激酶与DNA聚合酶α的相互作用可调节复制进程。  |  Hadjicharalambous, A., et al. 2023. Wellcome Open Res. 8: 327. PMID: 37766847
    8. 人类DNA聚合酶α-引物酶中的RNA引物和传递受柔性和分配合成调节。  |  Cordoba, JJ., et al. 2023. J Mol Biol. 435: 168330. PMID: 37884206
    9. DNA聚合酶α-引物酶催化结构中引物合成的机理模型。  |  Mullins, EA., et al. 2024. Nat Struct Mol Biol. 31: 777-790. PMID: 38491139
    10. DNA聚合酶α辅助因子C1C2作为引物识别蛋白发挥作用。  |  Pritchard, CG., et al. 1983. J Biol Chem. 258: 9810-9. PMID: 6224785

    订购信息

    产品名称产品编号规格价格数量收藏夹

    DNA pol α 抗体 (G-5)

    sc-365327
    200 µg/ml
    		$316.00

    DNA pol α (G-5): m-IgGκ BP-HRP 套装

    sc-552008
    200 µg Ab; 40 µg BP
    		$354.00

    DNA pol α (G-5) 中和勝肽

    sc-365327 P
    100 µg/0.5 ml
    		$68.00

    What application is the blocking peptide sc-365327 P appropriate for?

    Asked by: jerojero
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from Very nice and strong band by WB in HeLa nuclearVery nice and strong band by WB in HeLa nuclear extract. -SCBT QC
    Date published: 2015-06-06
    Rated 4 out of 5 by from Performs well in WesternPerforms well in Western, good signal strength in K562 nuclear extract.. -SCBT QC
    Date published: 2015-04-06
    Rated 5 out of 5 by from Produced great immunofluorescence nuclearProduced great immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
    Date published: 2014-10-09
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    DNA pol α 抗体 (G-5) is rated 4.7 out of 5 by 3.
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