Date published: 2025-9-6

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DNA pol α 抗体 (G-12): sc-365039

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说明书
  • DNA pol α抗体(G-12)是小鼠单克隆IgG2a κ, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的DNA pol α的N-terminus附近的氨基酸3-31之间
  • 推荐用于 mouse, rat 和 human 来源的DNA pol α WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是DNA pol α Antibody (G-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与DNA pol α Antibody (G-12) 打包提供(请参阅下面的订购信息)。

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関連項目

DNA的复制、重组和修复是维持基因组稳定性所必需的过程,这些过程需要核酸外切酶的参与。在DNA复制过程中,这些酶参与冈崎片段的加工;而在DNA修复过程中,它们的功能是切除受损的DNA片段并纠正重组错配。这些核酸外切酶包括DNA聚合酶家族。DNA聚合酶α、β、δ和ε参与DNA的复制和修复。DNA聚合酶δ和DNA聚合酶ε是多亚基酶,其中DNA聚合酶δ由两个亚基组成:p125,它与滑动DNA夹蛋白PCNA相互作用;另一个是p50。核编码的DNA聚合酶γ是线粒体DNA复制所需的唯一DNA聚合酶。DNA聚合酶ζ在各种组织中普遍表达,并介导损伤诱导突变的细胞机制。DNA聚合酶θ是一种与ATP结合的DNA聚合酶-解旋酶,并参与链间交联的修复。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DNA pol α 抗体 (G-12) 参考文献:

  1. 复制酶与衰老:DNA聚合酶α功能对细胞衰老事件的重要性。  |  Srivastava, VK. and Busbee, DL. 2002. Ageing Res Rev. 1: 443-63. PMID: 12067597
  2. DNA聚合酶α-引物酶的引物利用率受其与Mcm10p相互作用的影响。  |  Fien, K., et al. 2004. J Biol Chem. 279: 16144-53. PMID: 14766746
  3. 钙调蛋白在NRK细胞增殖激活过程中调节DNA聚合酶α的活性。  |  López-Girona, A., et al. 1992. Biochem Biophys Res Commun. 184: 1517-23. PMID: 1590809
  4. DNA聚合酶α/引物酶辅助蛋白的作用机制。  |  Goulian, M. and Heard, CJ. 1990. J Biol Chem. 265: 13231-9. PMID: 2376593
  5. DNA聚合酶-α通过细胞质RNA:DNA合成调节I型干扰素的激活。  |  Starokadomskyy, P., et al. 2016. Nat Immunol. 17: 495-504. PMID: 27019227
  6. 人类DNA聚合酶α错配延伸的结构和功能研究。  |  Baranovskiy, AG., et al. 2022. Proc Natl Acad Sci U S A. 119: e2111744119. PMID: 35467978
  7. 哺乳动物DNA聚合酶α全酶在复制叉的前导链和滞后链上可能具有的功能。  |  Ottiger, HP. and Hübscher, U. 1984. Proc Natl Acad Sci U S A. 81: 3993-7. PMID: 6588375
  8. DNA聚合酶α活性与单链DNA模板的辅助蛋白。  |  Lamothe, P., et al. 1981. Proc Natl Acad Sci U S A. 78: 4723-7. PMID: 6946421
  9. DNA聚合酶α在复制蛋白-A的存在下克服了易出错的暂停位点。  |  Suzuki, M., et al. 1994. J Biol Chem. 269: 10225-8. PMID: 8144602
  10. 与DNA聚合酶α相关的DNA解旋酶:通过改良的免疫亲和色谱法分离。  |  Biswas, EE., et al. 1993. Biochemistry. 32: 13393-8. PMID: 8257676

订购信息

产品名称产品编号规格价格数量收藏夹

DNA pol α 抗体 (G-12)

sc-365039
200 µg/ml
$316.00

DNA pol α (G-12): m-IgG Fc BP-HRP 套装

sc-537763
200 µg Ab; 10 µg BP
$354.00

DNA pol α (G-12): m-IgGκ BP-HRP 套装

sc-535134
200 µg Ab; 40 µg BP
$354.00

DNA pol α (G-12): m-IgG2a BP-HRP 套装

sc-548113
200 µg Ab; 10 µg BP
$354.00

DNA pol α (G-12) 中和勝肽

sc-365039 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-365039 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Produced beautiful immunofluorescence nuclearProduced beautiful immunofluorescence nuclear and perinuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-11
Rated 5 out of 5 by from Produced positive Western Blot data of DNAProduced positive Western Blot data of DNA pol expression in HeLa and Jurkat nuclear extracts. -SCBT QC
Date published: 2014-01-06
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DNA pol α 抗体 (G-12) is rated 5.0 out of 5 by 2.
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