DGAT1抗体 (A-5) 推荐用于 human 来源的DGAT1 WB, IP, IF, IHC(P) 和 ELISA检测

DGAT1 抗体 (A-5): sc-271934

DGAT1抗体 (A-5) is rated 5.0 out of 5 by 4.
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说明书

    • 抗-DGAT1 抗体 A-5 是小鼠单克隆 IgG1 κ,DGAT1抗体, 在11篇文献中引用,规格为200 µg/ml
    • 免疫原氨基酸序列1-100位于human物种的DGAT1的N-terminus
    • DGAT1 抗体 (A-5) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 DGAT1
    • 抗-DGAT1 抗体 (A-5) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取DGAT1 (A-5): sc-271934免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG1 BP-HRP 是DGAT1 Antibody (A-5) for WB and IHC(P)首选应用二抗. 这些试剂现在以套装形式提供DGAT1 Antibody (A-5) (见下方订购信息).
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What is the difference between DGAT1 (A-5) and DGAT1 (G-6) antibody? Which one do you recommend for WB with Huh 7.5 cell?

Asked by: Katsuki
Thank you for your question. These are two different mouse monoclonal antibodies both suitable for Western blot of human DGAT1. They are different isotypes and have different epitopes. We have not tested either antibody in Huh 7.5 cells.
Answered by: Technical Support
Date published: 2018-11-30

Can it be used for WB with Huh 7.5 cells?

Asked by: Katsuki
Thank you for your question. This antibody, DGAT1 (A-5): sc-271934 , is recommended for Western blot. We have not tested the antibody specifically on Huh 7.5 cells.
Answered by: Technical Support
Date published: 2018-11-30

Can DGAT1 (A-5): sc-271934 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your question. Yes, DGAT1 (A-5): sc-271934 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from good resources use it at 1:200 for WB ,get a good resource is singer ana clear.
Date published: 2017-08-18
Rated 5 out of 5 by from Worked well for western blotting with human Worked well for western blotting with human DGAT1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-05-23
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmic Produced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from WB: clean bands in HeLa and Hep G2 whole WB: clean bands in HeLa and Hep G2 whole cell lysates. -SCBT QC
Date published: 2015-03-09
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