DDX37 Double Nickase Plasmid (h): sc-412866-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDX37 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DDX37 Double Nickase Plasmid (h) and DDX37 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DHX37. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX37 Double Nickase Plasmid (h) | sc-412866-NIC | 20 µg | $410.00 | |||
DDX37 Double Nickase Plasmid (h2) | sc-412866-NIC-2 | 20 µg | $410.00 |
DHX37 encodes the human DEAD-box RNA helicase DDX37, a nucleolar enzyme that remodels RNA–protein complexes during ribosome biogenesis and pre-rRNA processing. DDX37 participates in ATP-dependent RNA unwinding steps required for small subunit maturation, linking its activity to nucleolar homeostasis, translation capacity, and cell-cycle progression. Perturbation of RNA helicase–mediated ribosome assembly can trigger nucleolar stress signaling and broad transcriptome and proteome changes. DHX37 has been implicated in disorders of gonadal development and is also of interest in cancer biology contexts where ribosome biogenesis and RNA processing pathways are frequently rewired.
DDX37 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DHX37 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DHX37. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DHX37 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DHX37-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.