Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DBH Double Nickase Plasmid (h): sc-402441-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DBH Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DBH Double Nickase Plasmid (h) and DBH Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DBH. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DBH Antibody (A-9): sc-365710
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DBH Double Nickase Plasmid (h) | sc-402441-NIC | 20 µg | $410.00 | |||
DBH Double Nickase Plasmid (h2) | sc-402441-NIC-2 | 20 µg | $410.00 |
Dopamine beta-hydroxylase (DBH) is a copper-dependent monooxygenase that catalyzes the conversion of dopamine to norepinephrine within secretory vesicles of sympathetic neurons and adrenal chromaffin cells. By controlling the dopamine:norepinephrine balance, DBH influences catecholamine biosynthesis, adrenergic neurotransmission, and stress-responsive neuroendocrine signaling. DBH activity intersects with tyrosine metabolism and vesicular monoamine handling pathways, shaping downstream adrenergic receptor signaling and cyclic nucleotide–linked responses. Genetic and regulatory variation in DBH has been associated with disorders of autonomic function and neuropsychiatric phenotypes, making it a relevant target for mechanistic studies of catecholamine homeostasis.
DBH Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DBH locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DBH. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DBH function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DBH-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.