Cytokeratin 6A CRISPR/Cas9 KO Plasmid (m): sc-421354
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cytokeratin 6A CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Cytokeratin 6A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cytokeratin 6A CRISPR/Cas9 KO Plasmid (m) | sc-421354 | 20 µg | $397.00 |
Overview
Krt6a encodes cytokeratin 6A, a type II intermediate filament protein that heterodimerizes with type I keratins to build the keratin cytoskeleton in stratified epithelia. It is rapidly induced during wound repair, mechanical stress, and inflammatory signaling, supporting keratinocyte migration, proliferation, and barrier reinforcement. Cytokeratin 6A integrates with cytoskeletal remodeling programs and junctional dynamics, influencing epithelial integrity and tissue resilience. Altered Krt6a expression is associated with hyperproliferative epidermal states and keratinization defects, making it relevant to studies of skin homeostasis and epithelial stress responses in mouse models.
Cytokeratin 6A CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Krt6a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Krt6a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Krt6a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Cytokeratin 6A protein expression.
This CRISPR knockout system enables efficient generation of Krt6a-deficient cell models for investigation of Cytokeratin 6A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Krt6a exon(s) critical for Cytokeratin 6A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Krt6a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Cytokeratin 6A CRISPR/Cas9 KO Plasmid (m) and Cytokeratin 6A CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Krt6a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Cytokeratin 6A HDR Plasmid (m) and Cytokeratin 6A HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Krt6a homology arms to support homology-directed repair at defined Krt6a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.