Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CYPOR Double Nickase Plasmid (h): sc-402375-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CYPOR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CYPOR Double Nickase Plasmid (h) and CYPOR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting POR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CYPOR Antibody (F-10): sc-25270
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYPOR Double Nickase Plasmid (h) | sc-402375-NIC | 20 µg | $410.00 | |||
CYPOR Double Nickase Plasmid (h2) | sc-402375-NIC-2 | 20 µg | $410.00 |
Human POR encodes cytochrome P450 oxidoreductase (CYPOR), an essential flavoprotein that transfers electrons from NADPH to microsomal cytochrome P450 enzymes in the endoplasmic reticulum. This activity supports xenobiotic metabolism, steroid hormone biosynthesis, fatty acid oxidation, and broader redox homeostasis by enabling CYP-dependent monooxygenase reactions. POR function therefore interfaces with drug metabolism pathways and endocrine steroidogenic networks that influence cellular stress responses and metabolic phenotypes. Genetic or functional perturbation of POR is relevant to disorders of steroidogenesis and variable pharmacogenomic responses, making it a useful target for mechanistic studies in hepatocyte-like and steroidogenic cell models.
CYPOR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the POR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within POR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt POR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of POR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.