Date published: 2025-9-6

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cyclin G1 抗体 (F-5): sc-8016

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说明书
  • cyclin G1 抗体 F-5 是小鼠单克隆 IgG1 κ,cyclin G1抗体, 在11篇文献中引用,规格为200 µg/ml
  • 免疫human来源cyclin G1位于N-terminus 的1-46氨基酸
  • cyclin G1 抗体 (F-5) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 cyclin G1
  • 抗cyclin G1抗体(F-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取cyclin G1 (F-5): sc-8016免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 cyclin G1 抗体 (F-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与cyclin G1 抗体 (F-5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Cyclin G1 抗体(F-5)是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学和酶联免疫吸附等方法检测人类来源的 Cyclin G1。Cyclin G1 (F-5) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。Cyclin G1 在细胞周期调节中起着至关重要的作用,特别是在从 G1 期到 S 期的过渡中,cyclin G1 参与控制细胞增殖。细胞周期蛋白G1对于细胞周期的正常进行至关重要,它有助于确保细胞仅在准备就绪时复制DNA,从而防止可能导致癌症的细胞无序分裂。细胞周期蛋白G1的特征在于其典型的N端细胞周期蛋白盒和C端与表皮生长因子受体的酪氨酸磷酸化位点同源的域。生长刺激后三个小时内,细胞周期蛋白G1的表达就会开始,且不受细胞周期的明显影响,一直保持高水平,这与其他细胞周期蛋白的表达模式不同。细胞周期蛋白G2与细胞周期蛋白G1的氨基酸序列相似度为53%,在S期后期达到表达峰值,进一步凸显了这些蛋白质在细胞周期动态中发挥的独特调节作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

cyclin G1 抗体 (F-5) 参考文献:

  1. D 型细胞周期蛋白与多种蛋白激酶以及 DNA 复制和修复因子 PCNA 相关联。  |  Xiong, Y., et al. 1992. Cell. 71: 505-14. PMID: 1358458
  2. 哺乳动物 D 型 G1 细胞周期蛋白的非典型催化亚基(p34PSK-J3/cdk4)的鉴定和特性。  |  Matsushime, H., et al. 1992. Cell. 71: 323-34. PMID: 1423597
  3. 有证据表明,在高等真核生物中,G1-S 和 G2-M 转变是由不同的 cdc2 蛋白控制的。  |  Fang, F. and Newport, JW. 1991. Cell. 66: 731-42. PMID: 1652371
  4. [小鼠卵巢中细胞周期蛋白 G1 的表达及与卵泡发育的关系]。  |  Liu, ZH., et al. 2006. Sichuan Da Xue Xue Bao Yi Xue Ban. 37: 893-7. PMID: 17236588
  5. 人类细胞周期蛋白 E,一种与 CDC2 基因家族两个成员相互作用的新型细胞周期蛋白。  |  Koff, A., et al. 1991. Cell. 66: 1217-28. PMID: 1833068
  6. 哺乳动物成纤维细胞开始 DNA 复制时需要 Cyclin A。  |  Girard, F., et al. 1991. Cell. 67: 1169-79. PMID: 1836977
  7. 人类细胞周期蛋白 A 是腺病毒 E1A 相关蛋白 p60,其行为与细胞周期蛋白 B 不同。  |  Pines, J. and Hunter, T. 1990. Nature. 346: 760-3. PMID: 2143810
  8. 细胞周期蛋白 G1 通过磷脂肌醇 3- 激酶/Akt 信号介导的上皮-间质转化促进了肝癌的进展。  |  Wen, W., et al. 2012. Hepatology. 55: 1787-98. PMID: 22271581
  9. 沉默细胞周期蛋白 G1 的表达可通过诱导细胞凋亡增强肝细胞癌在体外和体内的放射敏感性  |  Xu, G., et al. 2021. Radiat Res. 195: 378-384. PMID: 33543294
  10. 细胞周期蛋白 G1 通过 CDK5 激活诱导近端肾小管细胞不良分化和肾脏纤维化。  |  Taguchi, K., et al. 2022. J Clin Invest. 132: PMID: 36453545
  11. 细胞周期蛋白 G:一种新的哺乳动物细胞周期蛋白,与裂殖酵母 Cig1 同源。  |  Tamura, K., et al. 1993. Oncogene. 8: 2113-8. PMID: 8336937
  12. 细胞周期蛋白 G1 和细胞周期蛋白 G2 是一个新的细胞周期蛋白家族,具有不同的组织特异性和细胞周期调控表达。  |  Horne, MC., et al. 1996. J Biol Chem. 271: 6050-61. PMID: 8626390

订购信息

产品名称产品编号规格价格数量收藏夹

cyclin G1 抗体 (F-5)

sc-8016
200 µg/ml
$316.00

cyclin G1 (F-5): m-IgG Fc BP-HRP 套装

sc-526507
200 µg Ab; 10 µg BP
$354.00

cyclin G1 (F-5): m-IgG1 BP-HRP 套装

sc-531880
200 µg Ab; 20 µg BP
$354.00

cyclin G1 抗体 (F-5) AC

sc-8016 AC
500 µg/ml, 25% agarose
$416.00

cyclin G1 抗体 (F-5) HRP

sc-8016 HRP
200 µg/ml
$316.00

cyclin G1 抗体 (F-5) FITC

sc-8016 FITC
200 µg/ml
$330.00

cyclin G1 抗体 (F-5) PE

sc-8016 PE
200 µg/ml
$343.00

cyclin G1 抗体 (F-5) Alexa Fluor® 488

sc-8016 AF488
200 µg/ml
$357.00

cyclin G1 抗体 (F-5) Alexa Fluor® 546

sc-8016 AF546
200 µg/ml
$357.00

cyclin G1 抗体 (F-5) Alexa Fluor® 594

sc-8016 AF594
200 µg/ml
$357.00

cyclin G1 抗体 (F-5) Alexa Fluor® 647

sc-8016 AF647
200 µg/ml
$357.00

cyclin G1 抗体 (F-5) Alexa Fluor® 680

sc-8016 AF680
200 µg/ml
$357.00

cyclin G1 抗体 (F-5) Alexa Fluor® 790

sc-8016 AF790
200 µg/ml
$357.00

Cyclin G1 (F-5): sc-8016 200 ug/ml is a trash.I used this antibody do western blot, this antibody never works in my experiments though other tag antibody works. Also, I validated my samples by doing mass spec, confirmed it is cyclin G1.

Asked by: Shouqing Cui
Thank you for contacting Santa Cruz Biotechnology. We are sorry to hear the antibody did not work has expected . To troubleshoot the antibody please contact technical service by email scbt@scbt.com or call (800)-457-3801. Please provide your order number and lot number.
Answered by: BlakeJ
Date published: 2023-05-18

Is Cyclin G1 (F-5): sc-8016 200 ug/ml used for Western Blot? I tried this antibody do western blot, this antibody never works in my experiments though other tag antibody works. Is there specific different for sc-8016 and sc-8016 HRP ?

Asked by: Shouqing Cui
Thank you for your question. Please contact technical service to trouble shoot at (800)-457-3801 or email scbt@scbt.com
Answered by: BlakeJ
Date published: 2022-11-17

I am using cyclin G1 (F-5): sc-8016 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from WBに適した抗体Western blottingに用いましたが、特異的でクリアなバンドを見ることができて満足でした。
Date published: 2019-07-31
Rated 4 out of 5 by from okWeak signal of a single band at ~37 kDa in HeLa whole cell lysate. Strong non-specific band seen at ~41 kDa.
Date published: 2018-04-26
Rated 4 out of 5 by from a good antibody for WBThis antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from Good nuclear staining with paraffinGood nuclear staining with paraffin-embedded human colon samples. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Works very nicely for IF on human cancerWorks very nicely for IF on human cancer cell lines. -SCBT Publication Review
Date published: 2015-01-19
Rated 5 out of 5 by from Clean blot with single strong band at expectedClean blot with single strong band at expected size with recombinant His-tagged cyclin G1. -SCBT QC
Date published: 2014-10-03
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cyclin G1 抗体 (F-5) is rated 4.7 out of 5 by 6.
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