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CT-R Antibody (2F7) - Western Blotting - Image 348448
CT-R 抗体 (2F7): sc-293299. 人重组 CT-R 融合蛋白的 Western 印迹分析.
CT-R Antibody (2F7) - Western Blotting - Image 348448
CT-R 抗体 (2F7): sc-293299. 人重组 CT-R 融合蛋白的 Western 印迹分析.
CT-R 抗体 (2F7): sc-293299. 人重组 CT-R 融合蛋白的 Western 印迹分析.

CT-R 抗体 (2F7): sc-293299

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说明书
  • CT-R 抗体 2F7 是小鼠单克隆 IgG2a κ，CT-R抗体, 在1篇文献中引用，规格为100 µg/ml
  • 免疫human物种的CT-R的氨基酸394-474
  • 推荐用于 human 来源的CT-R WB, IP 和 ELISA检测
  • 目前，我们还没有完成CT-R 抗体 (2F7)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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说明书与实验方案

関連項目

CT-R抗体（2F7）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和酶联免疫吸附测定（ELISA）检测人源CT-R蛋白。抗CT-R抗体（2F7）以非结合形式提供。CT-R蛋白（也称为降钙素受体）通过调节甲状腺分泌的肽激素降钙素的作用，在钙稳态中发挥关键作用。CT-R (2F7)抗体识别一组独特的七种跨膜蛋白，包括甲状旁腺激素、促胰液素和胰高血糖素的受体。CT-R蛋白主要位于破骨细胞和肾细胞的表面，CT-R（2F7）单克隆抗体通过与各种异源三聚体G蛋白结合，启动细胞内信号级联反应。这种相互作用激活多种信号通路，包括涉及腺苷酸环化酶和磷脂酶C的信号通路，它们对于调节骨吸收和血液中的钙水平至关重要。编码CT-R的基因具有多个外显子和较大的内含子，可产生多种剪接变体，这些变体具有独特的功能特性和组织分布。这些同源物优先与特定的G蛋白结合，从而招募不同的信号传导中间体，这对于精确调节钙稳态和整体代谢平衡至关重要。在破骨细胞和胚胎肾细胞中，降钙素与CT-R结合后，通过磷酸化Shc和HEF1等适配蛋白激活了丝裂原活化蛋白激酶Erk1/2和蛋白激酶C，这表明CT-R能够独立于蛋白激酶A和腺苷酸环化酶等传统途径来传递复杂的信号。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CT-R 抗体 (2F7) 参考文献:

  1. 蛋白激酶 C 可拮抗百日咳毒素敏感的降钙素受体与腺苷酸环化酶的耦合。  |  Shyu, JF., et al. 1999. Eur J Biochem. 262: 95-101. PMID: 10231369
  2. G蛋白偶联降钙素受体下游的p130(Cas)家族成员HEF1的细胞骨架依赖性酪氨酸磷酸化。降钙素诱导 HEF1, paxillin 和病灶粘附激酶结合。  |  Zhang, Z., et al. 1999. J Biol Chem. 274: 25093-8. PMID: 10455189
  3. 来自正常骨髓的人类破骨细胞中的新型降钙素受体基因。  |  Nishikawa, T., et al. 1999. FEBS Lett. 458: 409-14. PMID: 10570950
  4. 降钙素：发现, 开发和临床应用。  |  Copp, DH. 1994. Clin Invest Med. 17: 268-77. PMID: 7924003
  5. 小鼠脑降钙素受体互补脱氧核糖核酸的克隆和表征以及降钙素受体基因图谱的绘制。  |  Yamin, M., et al. 1994. Endocrinology. 135: 2635-43. PMID: 7988453
  6. 人类降钙素受体丰富亚型的克隆和特征描述。  |  Kuestner, RE., et al. 1994. Mol Pharmacol. 46: 246-55. PMID: 8078488
  7. 降钙素受体刺激 Shc 酪氨酸磷酸化和 Erk1/2 激活。Gi, 蛋白激酶 C 和钙参与其中。  |  Chen, Y., et al. 1998. J Biol Chem. 273: 19809-16. PMID: 9677414

订购信息

产品名称产品编号规格价格数量收藏夹

CT-R 抗体 (2F7)

sc-293299
100 µg/ml
$316.00

Hi, does this antibody can detect the Ctr1 protein by western blot in yeast (Candida species)? Thank you~ruei

Asked by: ruei
Thank you for your question. CT-R Antibody (2F7): sc-293299 was raised against amino acids 394-474 of CT-R of human origin and is only recommended for detection of CT-R of human origin by WB, IP and ELISA. It is not recommended for samples from yeast or other species.
Answered by: Tech Support Europe
Date published: 2020-07-02

Hi: Do you know if the anti CTR1 sc-293299 can be used in western blots to detect rat or mouse CTR1 for lysates of mouse and rat cell lines? Thanks, steve

Asked by: Steve Yanofsky
Thank you for your question. We have not yet tested this antibody, CT-R (2F7): sc-293299, in samples of mouse or rat origin and so those species are not recommended at this time. The antibody is raised against amino acids 394-474 of CT-R of human origin, which shares approximately 80% sequence identity with CT-R in mouse and rat. This is below the standard threshold where we would predict cross-reactivity in the absence of data. However, it is still significant enough that the antibody may also cross-react with those species.
Answered by: Technical Support
Date published: 2020-06-30

For Western Blot, is it recommended to use denatured or non-denatured conditions with CT-R (2F7): sc-293299 antibody?

Asked by: TinTin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Can be used for immunofluorescenceAntibody has been used to detect CT-R in differentiated RAW 264.7 cells by immunofluorescence in published research. -SCBT QC
Date published: 2023-09-14
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CT-R 抗体 (2F7) is rated 5.0 out of 5 by 1.
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