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CPTII 抗体 (G-5): sc-377294

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说明书
  • CPTII 抗体 G-5 是小鼠单克隆 IgG1 κ,CPTII抗体, 在10篇文献中引用,规格为200 µg/ml
  • 免疫human物种的CPTII的氨基酸51-350
  • CPTII 抗体 (G-5) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 CPTII
  • 抗CPTII抗体(G-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CPTII (G-5): sc-377294免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是CPTII Antibody (G-5) for WB applications. 的首选辅助检测试剂,这些试剂现与CPTII Antibody (G-5) 打包提供(请参阅下面的订购信息)。

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関連項目

CPTII抗体(G-5)是一种IgG1 κ小鼠单克隆CPTII抗体(也称为CPT2抗体、肉碱/胆碱乙酰转移酶抗体、肉碱棕榈酰转移酶II抗体、PT1抗体、CPTASE抗体或感染诱导的急性脑病-4(IIAE4)抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源性CPTII蛋白。CPTII抗体(G-5)既有非偶联型抗CPTII抗体形式,也有多种偶联型抗CPTII抗体形式,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。长链脂肪酸的线粒体β-氧化是由肉碱棕榈酰转移酶(CPT)I(外膜和洗涤剂不稳定)和II(内膜和洗涤剂稳定)以及肉碱载体的顺序作用引发的。CPTI催化长链脂肪酸从细胞质到线粒体的转运中的第一个反应,这是β-氧化的限速步骤。已知有两种类型的CPTI,即肝型(CPTIA)和肌型(CPTIB)同工酶。肌肉型蛋白主要在心脏和骨骼肌中表达。膜结合型CPTI(而非CPTII)可受到丙二酰辅酶A(CoA)的可逆性抑制。与CPTII不同,CPTI的催化功能需要膜完整性。此外,谷氨酸3和组氨酸5对于丙二酰CoA抑制和与肝CPTI的结合是必需的,但对于催化活性则不是必需的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CPTII 抗体 (G-5) 参考文献:

  1. 肉碱长链酰基转移酶活性的选择性调节。COT和CPT-II的动力学、抑制剂和活性位点。  |  Ramsay, RR. and Gandour, RD. 1999. Adv Exp Med Biol. 466: 103-9. PMID: 10709633
  2. 在酵母 Pichia pastoris 中表达的哺乳动物线粒体肉碱棕榈酰转移酶 I 和 II 的功能特征。  |  Woldegiorgis, G., et al. 2000. J Nutr. 130: 310S-314S. PMID: 10721894
  3. 一种新的无义突变与致死性新生儿CPTII缺乏症中的畸形有关。  |  McGill, JS., et al. 2012. Clin Dysmorphol. 21: 179-181. PMID: 22652984
  4. 线粒体肉碱棕榈酰基转移酶酶系统的组织间和物种间特征。  |  Woeltje, KF., et al. 1990. J Biol Chem. 265: 10714-9. PMID: 2355017
  5. 酮体生成的调节和肉碱棕榈酰转移酶的复兴。  |  McGarry, JD., et al. 1989. Diabetes Metab Rev. 5: 271-84. PMID: 2656156
  6. CPTII纯合突变患者在斋月禁食期间因长期禁食和间歇性高脂负荷而加重了严重的高氨血症性脑病,需要透析治疗。  |  Phowthongkum, P., et al. 2018. JIMD Rep. 41: 11-16. PMID: 29159461
  7. CPTII缺乏症原位心脏移植的成功。  |  Arnold, GL., et al. 2021. Mol Genet Metab. 133: 182-184. PMID: 34020866
  8. 人类肝脏线粒体肉碱棕榈酰基转移酶 I:其 cDNA 和染色体定位的特征以及基因的部分分析。  |  Britton, CH., et al. 1995. Proc Natl Acad Sci U S A. 92: 1984-8. PMID: 7892212
  9. 编码人类肌肉型肉碱棕榈酰转移酶 I 的 cDNA 和基因组克隆的分离与特征描述。  |  Yamazaki, N., et al. 1996. Biochim Biophys Acta. 1307: 157-61. PMID: 8679700
  10. 编码人类肌肉型肉碱棕榈酰转移酶 I 基因的结构特征。  |  Yamazaki, N., et al. 1997. FEBS Lett. 409: 401-6. PMID: 9224698
  11. 酵母表达的人心肌肉碱棕榈酰基转移酶 I 的功能研究。  |  Zhu, H., et al. 1997. Arch Biochem Biophys. 347: 53-61. PMID: 9344464
  12. 心脏线粒体肉碱酰肉碱转运酶系统的特征。  |  Pande, SV. and Parvin, R. 1976. J Biol Chem. 251: 6683-91. PMID: 977593

订购信息

产品名称产品编号规格价格数量收藏夹

CPTII 抗体 (G-5)

sc-377294
200 µg/ml
$316.00

CPTII (G-5): m-IgG Fc BP-HRP 套装

sc-527593
200 µg Ab; 10 µg BP
$354.00

CPTII (G-5): m-IgG1 BP-HRP 套装

sc-532966
200 µg Ab; 20 µg BP
$354.00

CPTII 抗体 (G-5) AC

sc-377294 AC
500 µg/ml, 25% agarose
$416.00

CPTII 抗体 (G-5) HRP

sc-377294 HRP
200 µg/ml
$316.00

CPTII 抗体 (G-5) FITC

sc-377294 FITC
200 µg/ml
$330.00

CPTII 抗体 (G-5) PE

sc-377294 PE
200 µg/ml
$343.00

CPTII 抗体 (G-5) Alexa Fluor® 488

sc-377294 AF488
200 µg/ml
$357.00

CPTII 抗体 (G-5) Alexa Fluor® 546

sc-377294 AF546
200 µg/ml
$357.00

CPTII 抗体 (G-5) Alexa Fluor® 594

sc-377294 AF594
200 µg/ml
$357.00

CPTII 抗体 (G-5) Alexa Fluor® 647

sc-377294 AF647
200 µg/ml
$357.00

CPTII 抗体 (G-5) Alexa Fluor® 680

sc-377294 AF680
200 µg/ml
$357.00

CPTII 抗体 (G-5) Alexa Fluor® 790

sc-377294 AF790
200 µg/ml
$357.00

ask for a FREE 10 µg sample of CPTII (G-5): sc-377294

Asked by: Zhenning
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with CPTII (G-5): sc-377294?

Asked by: Dr Ninau Qelp
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Clean and specificWorked well for WB (mouse heart, one band at expected size). Originally ordered nonconjugated antibody which could not be delivered until Monday, however, we'd have a lockdown on Friday due to Covid19. Contacted customer service on Thursday afternoon, they were able to get me the HRP conjugated antibody delivered by Friday 1pm, and I got the result right before lockdown. Very happy about the results, perfect for my paper revision. Thank you!
Date published: 2020-03-21
Rated 5 out of 5 by from Good for mouse tissuegave a strong and clean band of the right size from mouse tissue
Date published: 2018-12-04
Rated 5 out of 5 by from Good clear bands in WBAntibody (1:500) showed one clear band in WB of primary mouse hepatocyte protein lysates
Date published: 2018-01-10
Rated 5 out of 5 by from Produced positive Western blot data of CPTIIProduced positive Western blot data of CPTII expression in Hep G2 whole cell lysate. -SCBT QC
Date published: 2014-10-15
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CPTII 抗体 (G-5) is rated 5.0 out of 5 by 4.
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