Date published: 2025-9-7
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COX4 Antibody (F-8) - Western Blotting - Image 295850
COX4 抗体 (F-8): sc-376731. A-431 (A), MOLT-4 (B), Raji (C), MCF7 (D) 和 A-673 (E) 全细胞裂解液及人骨骼肌组织提取物 (F) 中 COX4 表达的 Western 印迹分析.
COX4 抗体 (F-8): sc-376731. A-431 (A), MOLT-4 (B), Raji (C), MCF7 (D) 和 A-673 (E) 全细胞裂解液及人骨骼肌组织提取物 (F) 中 COX4 表达的 Western 印迹分析.

COX4 抗体 (F-8): sc-376731

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寫評論提問

说明书
  • COX4 抗体 F-8 是小鼠单克隆 IgG1 κ，COX4抗体, 在69篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列86-169位于human物种的COX4的C-terminus
  • COX4 抗体 (F-8) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 COX4
  • 抗COX4抗体（F-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取COX4 (F-8): sc-376731的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 COX4 抗体 (F-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与COX4 抗体 (F-8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Cytochrome c Oxidase 抗体 包括 COX15, COX17, COX2, COX4, COX4I2, COX4NB, COX5a, COX5b, COX6b1和COX6c

COX4抗体（F-8）是一种IgG1 κ小鼠单克隆COX4抗体（也称为COX4I1抗体或COXIV抗体），可通过WB、IP、IF、IHC(P)和ELISA检测人源的COX4蛋白。COX4抗体（F-8）既可以是非偶联型抗COX4抗体形式，也可以是多种偶联型抗COX4抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。细胞色素c氧化酶（COX）作为呼吸链的末端氧化酶，利用细胞色素c作为电子供体驱动质子梯度穿过线粒体内膜。哺乳动物的COX脱辅酶是一个异聚体，由三个线粒体编码的催化亚基和几个核基因编码的结构亚基组成。COX含有两个铁配位点和两个铜配位点。细胞色素c氧化酶IV（COX4）是COX的核编码亚基，可能在调节COX活性中发挥作用。COX4在成年人体组织中普遍表达，在胰腺中表达水平最高，在心脏、骨骼肌和胎盘中表达水平中等。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

COX4 抗体 (F-8) 参考文献:

  1. COX4 基因的 5' 区域包含一个新的重叠基因 NOC4。  |  Bachman, NJ., et al. 1999. Mamm Genome. 10: 506-12. PMID: 10337626
  2. 哺乳动物细胞色素 c 氧化酶亚单位 IV 同工型。  |  Hüttemann, M., et al. 2001. Gene. 267: 111-23. PMID: 11311561
  3. 小鼠红细胞白血病（MEL）细胞中 c-myc, B22, COX II 和 COX IV 基因的分化抑制。  |  Vizirianakis, IS., et al. 2002. Biochem Pharmacol. 63: 1009-17. PMID: 11911854
  4. 人类细胞色素 c 氧化酶亚基 IV 基因的快速进化。  |  Lomax, MI., et al. 1992. Proc Natl Acad Sci U S A. 89: 5266-70. PMID: 1319058
  5. 细胞色素 c 氧化酶 IV[1--3]的研究。亚基 II 的初级结构和功能。  |  Steffens, GJ. and Buse, G. 1979. Hoppe Seylers Z Physiol Chem. 360: 613-9. PMID: 220175
  6. Cox4和线粒体Hsp70复合物在细胞色素c氧化酶的组装中起着重要作用。  |  Böttinger, L., et al. 2013. Mol Biol Cell. 24: 2609-19. PMID: 23864706
  7. 分离编码人细胞色素 c 氧化酶亚单位 IV 的 cDNA 克隆。  |  Zeviani, M., et al. 1987. Gene. 55: 205-17. PMID: 2444497
  8. 在线粒体COX4-1缺乏症中，通过HIF-1α上调COX4-2。  |  Douiev, L., et al. 2021. Cells. 10: PMID: 33672589
  9. COX4-1促进线粒体超复合体的组装，并限制抗辐射性GBM中活性氧物种的产生。  |  Oliva, CR., et al. 2022. Cell Stress. 6: 45-60. PMID: 35478774
  10. 细胞色素c氧化酶IV同工型1（COX4-1）通过调节线粒体功能来调控滋养细胞增殖、迁移和侵袭。  |  Yu, J., et al. 2024. Placenta. 151: 48-58. PMID: 38718733
  11. 灵长类动物的线粒体 DNA 序列：进化的速度和模式。  |  Brown, WM., et al. 1982. J Mol Evol. 18: 225-39. PMID: 6284948
  12. 编码细胞色素 c 氧化酶亚单位 IV 的基因映射到小鼠的第 8 号染色体上。  |  Makris, GJ., et al. 1996. Mamm Genome. 7: 789-90. PMID: 8854875

订购信息

产品名称产品编号规格价格数量收藏夹

COX4 抗体 (F-8)

sc-376731
200 µg/ml
$316.00

COX4 (F-8): m-IgG Fc BP-HRP 套装

sc-529928
200 µg Ab; 10 µg BP
$354.00

COX4 (F-8): m-IgGκ BP-HRP 套装

sc-523068
200 µg Ab, 40 µg BP
$354.00

COX4 抗体 (F-8) AC

sc-376731 AC
500 µg/ml, 25% agarose
$416.00

COX4 抗体 (F-8) HRP

sc-376731 HRP
200 µg/ml
$316.00

COX4 抗体 (F-8) FITC

sc-376731 FITC
200 µg/ml
$330.00

COX4 抗体 (F-8) PE

sc-376731 PE
200 µg/ml
$343.00

COX4 抗体 (F-8) Alexa Fluor® 488

sc-376731 AF488
200 µg/ml
$357.00

COX4 抗体 (F-8) Alexa Fluor® 546

sc-376731 AF546
200 µg/ml
$357.00

COX4 抗体 (F-8) Alexa Fluor® 594

sc-376731 AF594
200 µg/ml
$357.00

COX4 抗体 (F-8) Alexa Fluor® 647

sc-376731 AF647
200 µg/ml
$357.00

COX4 抗体 (F-8) Alexa Fluor® 680

sc-376731 AF680
200 µg/ml
$357.00

COX4 抗体 (F-8) Alexa Fluor® 790

sc-376731 AF790
200 µg/ml
$357.00

Hello, have you tested this antibody by chance in mouse? I am look for a nuclear control for western blots.

Asked by: westerns001
Thank you for your question. COX4 Antibody (F-8) is only recommended for detection of COX4 of human origin by WB, IP, IF, IHC(P) and ELISA. Based on our tests we do not recommend it for murine cells or tissues.
Answered by: Tech Support Europe
Date published: 2022-08-15

Can you recommend a positive control to use with COX4 (F-8): sc-376731 monoclonal antibody for Western blot?

Asked by: jenni
We recommend A-431 (sc-2201), MOLT-4 (sc-2201), Raji (sc-364236), MCF7 (sc-2206) and A-673 (sc-2414) whole cell lysates to use as positive control.
Answered by: Technical Support 8
Date published: 2017-01-10
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Rated 5 out of 5 by from Excellent functionI bought this product. It's very easy to use. I recommend you to buy this product.
Date published: 2023-02-01
Rated 5 out of 5 by from It worked greatIt works great without background. The dilution I used was 1:500.
Date published: 2021-10-25
Rated 4 out of 5 by from WB LCLsL'anticorpo è stato usato per WB su linfoblasti e ha dato un buon segnale specifico!
Date published: 2021-04-30
Rated 1 out of 5 by from No band!I used fresh antibody dilution (1:100 and 1:50) on 10ug HUVEC cell lysate and I did not get any signal in western blotting. Conversely, I detected a sharp band with anti-HADHA (sc-374497) on the same blot.
Date published: 2019-11-12
Rated 1 out of 5 by from too many nonspecific bandsI have been using COX4 antibody from Santa Cruz for mitochondrial fractionation (Western blot). Since the older product was no longer available, I ordered this version of antibody. And there are just too many of the nonspecific bands all over, including 30 kD band signal, which is virtually the strongest signal in my membrane. I wonder how others use this antibody.
Date published: 2018-05-09
Rated 1 out of 5 by from no bandDespite high sample input (up to 100µg protein per well) and fresh antibody dilution (1:100) I received not a single band in either whole cell lysate or mitochondrial fraction
Date published: 2018-03-01
Rated 4 out of 5 by from It works!Got the sample and tried it out. The results shows strong band at right position.
Date published: 2017-12-21
Rated 5 out of 5 by from very goodI tested it in C42B cell line. Works very good. A single band.
Date published: 2017-09-27
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COX4 抗体 (F-8) is rated 4.2 out of 5 by 28.
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