Date published: 2025-9-7
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Cofilin 1 Antibody (5) - Western Blotting - Image 353963
Cofilin 1 抗体 (5): sc-53934. NIH/3T3 (A), MCF7 (B), HEK293 (C), Caco-2 (D), Raji (E) 和 A-431 (F) 全细胞裂解液中 Cofilin 1 表达的 Western 印迹分析.
Cofilin 1 抗体 (5): sc-53934. NIH/3T3 (A), MCF7 (B), HEK293 (C), Caco-2 (D), Raji (E) 和 A-431 (F) 全细胞裂解液中 Cofilin 1 表达的 Western 印迹分析.

Cofilin 1 抗体 (5): sc-53934

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说明书
  • Cofilin 1 抗体 5 是小鼠单克隆 IgG2b κ，Cofilin 1抗体, 在28篇文献中引用，规格为200 µg/ml
  • 针对全长肌动蛋白结合蛋白1（Cofilin 1来源）的抗体
  • Cofilin 1 抗体 (5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Cofilin 1
  • Cofilin 1抗体（5）可与Alexa Fluor® 546或Alexa Fluor® 594结合，用于WB（RGB）、IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cofilin 1 (5): sc-53934的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 Cofilin 1 抗体 (5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Cofilin 1 抗体 (5) 组合成套装提供。 (请参阅下面的订购信息)。

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研究信息

関連項目

此外 Cofilin 抗体包括 Cofilin 1 和 Cofilin 2

Cofilin 1 抗体 (5) 是一种小鼠单克隆 IgG2b kappa 轻链抗体，可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF)、免疫组织化学和酶联免疫吸附试验 (ELISA) 检测小鼠、大鼠和人源的 Cofilin 1 蛋白。抗 Cofilin 1 抗体 (5) 有非结合型和多种 Alexa Fluor® 结合型。Cofilin 1 在调节肌动蛋白动力学方面起着至关重要的作用，而肌动蛋白动力学对包括运动、分裂和凋亡在内的各种细胞过程都至关重要。Cofilin 1 主要位于细胞质中，在细胞质中，Cofilin 1 与丝状肌动蛋白（F-actin）相互作用，促进肌动蛋白丝的分解，从而促进细胞骨架的快速重塑。Cofilin 1 与 F-肌动蛋白结合的能力对维持细胞的完整性和功能至关重要，可使细胞对环境线索做出反应，并相应地调整形状和运动。此外，Cofilin 1 的活性受到磷酸化的调控；当被 LIM 激酶 1 磷酸化时，Cofilin 1 就会失去活性，无法结合 F-肌动蛋白，这凸显了翻译后修饰在控制功能方面的重要性。这种调控对细胞迁移和组织重塑等过程至关重要，因此 Cofilin 1 在正常生理和疾病状态中都扮演着重要角色。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cofilin 1 抗体 (5) 参考文献:

  1. 通过快速去磷酸化和转运至质膜，cofilin 参与了溶血酶素引发的中性粒细胞样 HL-60 细胞的活化。  |  Nagaishi, K., et al. 1999. J Biochem. 125: 891-8. PMID: 10220580
  2. LIM激酶1。  |  Stanyon, CA. and Bernard, O. 1999. Int J Biochem Cell Biol. 31: 389-94. PMID: 10224665
  3. 酿酒酵母中两种肌动蛋白结合蛋白（cofilin 和 Aip1）的合作。  |  Iida, K. and Yahara, I. 1999. Genes Cells. 4: 21-32. PMID: 10231390
  4. 人类恶性细胞中的Cofilin-1和其他ADF/Cofilin超家族成员。  |  Shishkin, S., et al. 2016. Int J Mol Sci. 18: PMID: 28025492
  5. 储存血小板中由Cofilin-1诱导的肌动蛋白重组。  |  Dasgupta, SK. and Thiagarajan, P. 2020. Transfusion. 60: 806-814. PMID: 32159862
  6. Cofilin-1参与严重再生障碍性贫血患者骨髓树突状细胞的超功能。  |  Sun, Y., et al. 2022. J Cell Mol Med. 26: 3460-3470. PMID: 35579089
  7. Cofilin-1通过促进内质网应激介导的铁死亡诱导急性肾损伤。  |  Lin, S., et al. 2023. Hum Cell. 36: 1928-1937. PMID: 37548903
  8. Cofilin-1的N-端α-氨基SUMO化对其调节肌动蛋白解聚至关重要。  |  Weng, W., et al. 2023. Nat Commun. 14: 5688. PMID: 37709794
  9. 科菲林的 Ser-3 磷酸化可调节其在肌动蛋白上的基本功能。  |  Moriyama, K., et al. 1996. Genes Cells. 1: 73-86. PMID: 9078368
  10. cofilin/destrin在肌动蛋白细胞骨架重组过程中对压力和细胞刺激的反应中的作用。  |  Yahara, I., et al. 1996. Cell Struct Funct. 21: 421-4. PMID: 9118250
  11. Cofilin 可促进体内肌动蛋白丝的快速周转。  |  Lappalainen, P. and Drubin, DG. 1997. Nature. 388: 78-82. PMID: 9214506
  12. Cofilin改变F-肌动蛋白的扭曲：对肌动蛋白丝动力学和细胞功能的影响。  |  McGough, A., et al. 1997. J Cell Biol. 138: 771-81. PMID: 9265645

订购信息

产品名称产品编号规格价格数量收藏夹

Cofilin 1 抗体 (5)

sc-53934
200 µg/ml
$316.00

Cofilin 1 (5): m-IgG Fc BP-HRP 套装

sc-525621
200 µg Ab; 10 µg BP
$354.00

Cofilin 1 抗体 (5) Alexa Fluor® 546

sc-53934 AF546
200 µg/ml
$357.00

Cofilin 1 抗体 (5) Alexa Fluor® 594

sc-53934 AF594
200 µg/ml
$357.00

Cofilin 1 抗体 (5) Alexa Fluor® 680

sc-53934 AF680
200 µg/ml
$357.00

Cofilin 1 抗体 (5) Alexa Fluor® 790

sc-53934 AF790
200 µg/ml
$357.00

Could sc-53934 be used for FCM (Flow Cytometry)? Thanks!

Asked by: sme1203
Thank you for your question. We have not tested this antibody, Cofilin 1 (5): sc-53934, for flow cytometry (FCM), so it is not a recommended application at this time.
Answered by: Technical Support
Date published: 2017-07-25

Can Cofilin 1 (5): sc-53934 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, Cofilin 1 (5): sc-53934 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 7
Date published: 2017-01-13

What is the total volume provided of Cofilin 1 (5):sc-53934 monoclonal antibody?

Asked by: jenni
Thank you for your question. This antibody is provided as 200 µg in 1 ml total volume of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 7
Date published: 2017-01-13
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Rated 5 out of 5 by from SI have bought this one month ago.It's really working
Date published: 2023-11-17
Rated 5 out of 5 by from Cofilin 1 Antibody (5): sc-53934works well with western blot analysis of human gland tissues
Date published: 2021-02-13
Rated 1 out of 5 by from Poor signal for endogenous Cofilin in HEK293 cellsThis antibody was tested alongside sc-376476 and sc-166958, but did not give any signal using 1:1000 dilution on HEK293T cells extract. Only a faint band was visible at approx 35kDa, for this antibody and all Cofilin antibodies tested. 50ug of proteins were separated on a 10% SDS-PAGE and transferred on nitrocellulose membrane that was blocked using 5% milk in TBS-T. Antibody incubation was done for 16h at 4 degrees Cielcius.
Date published: 2019-01-24
Rated 5 out of 5 by from Great antibody for western blottingWe used 1:1000 on western blotting from NPCs. it worked great
Date published: 2018-07-19
Rated 1 out of 5 by from no bands1:1000 in 5%dry milk ON. 20µg protein applied. Clarity ECL developement on Chemidoc. No bands
Date published: 2018-04-09
Rated 4 out of 5 by from Good antibodyThis Cofilin1 antibody worked well, bit required to be used at a high concentration; 1:200!
Date published: 2018-03-28
Rated 2 out of 5 by from WBCofillin1 (5) did not worked in the WB of mouse lung primary fibroblast. We used anti-mouse IgG as the secondary antibody. Maybe the problem was the secondary antibody.
Date published: 2018-03-28
Rated 5 out of 5 by from Excellent readoutWe tested this antibody using mouse whole brain lysates on western blotting. The signal is strong and clean. Highly recommended.
Date published: 2018-03-27
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Cofilin 1 抗体 (5) is rated 4.3 out of 5 by 23.
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