CNG-1 CRISPR/Cas9 KO Plasmid (m): sc-419713
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CNG-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CNG-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CNG-1 CRISPR/Cas9 KO Plasmid (m) | sc-419713 | 20 µg | $397.00 |
Overview
Cnga1 encodes the alpha subunit of the cyclic nucleotide-gated channel CNG-1, a key component of the phototransduction machinery in mouse rod photoreceptors. By forming cGMP-gated cation channels in the outer segment plasma membrane, CNG-1 links light-driven changes in cGMP to Na⁺/Ca²⁺ influx, shaping membrane potential and downstream synaptic signaling. This channel function interfaces with cGMP metabolism, Ca²⁺ homeostasis, and adaptation mechanisms that regulate visual sensitivity. Disruption of CNGA1-dependent signaling is associated with rod dysfunction and retinal degenerative phenotypes, making it relevant for studies of inherited retinal disease mechanisms.
CNG-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cnga1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cnga1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cnga1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CNG-1 protein expression.
This CRISPR knockout system enables efficient generation of Cnga1-deficient cell models for investigation of CNG-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cnga1 exon(s) critical for CNG-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cnga1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CNG-1 CRISPR/Cas9 KO Plasmid (m) and CNG-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cnga1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CNG-1 HDR Plasmid (m) and CNG-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cnga1 homology arms to support homology-directed repair at defined Cnga1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.