Date published: 2025-9-5

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claudin-5 抗体 (A-12): sc-374221

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说明书
  • claudin-5 抗体 A-12 是小鼠单克隆 IgG1 κ,claudin-5抗体, 在30篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列167-218位于human物种的claudin-5的C-terminus
  • claudin-5 抗体 (A-12) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 claudin-5
  • 抗claudin-5抗体(A-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取claudin-5 (A-12): sc-374221免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP是claudin-5 Antibody (A-12) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与claudin-5 Antibody (A-12) 打包提供(请参阅下面的订购信息)。

快捷链接

関連項目

claudin-5 抗体(A-12)是一种 IgG1 κ 小鼠单克隆 claudin-5 抗体(也称为 CLDN5 抗体),可通过 WB、IP、IF 和 ELISA 检测人源 claudin-5 蛋白。claudin-5 抗体(A-12)既可以是非偶联的抗 claudin-5 抗体形式,也可以是多种偶联形式的抗 claudin-5 抗体,包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。claudin 超家族由人体内许多结构相关的蛋白质组成。这些蛋白质是细胞旁转运中紧密连接的重要结构和功能组分。Claudins 分布于所有具有紧密连接的组织中的上皮细胞和内皮细胞。已知有三类蛋白质定位于紧密连接,包括 claudins、Occludin 和连接粘附分子。Claudins 由四个跨膜结构域和两个细胞外环组成,构成紧密连接链。Claudin 的表达严格限制于不同组织的特定区域,并可能在通过紧密连接的跨细胞转运中发挥重要作用。Claudin-5 表达于大鼠肝脏的内皮连接和胰腺腺泡细胞的连接中。人 Claudin-5 在成人肺、心脏和骨骼肌中大量表达,并在口面指(趾)端综合征患者中缺失,该病症的特征是腭裂、面部畸形和心脏流出道缺陷。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

claudin-5 抗体 (A-12) 参考文献:

  1. 紧密连接屏障中的跨膜蛋白  |  Fanning, AS., et al. 1999. J Am Soc Nephrol. 10: 1337-45. PMID: 10361874
  2. 产气荚膜梭状芽孢杆菌肠毒素能与紧密连接整体膜蛋白 Claudin-3 的第二个细胞外环结合。  |  Fujita, K., et al. 2000. FEBS Lett. 476: 258-61. PMID: 10913624
  3. 大鼠肝脏, 胰腺和肠道中克劳丁蛋白 2, 3, 4 和 5 的表达和亚细胞定域的异质性。  |  Rahner, C., et al. 2001. Gastroenterology. 120: 411-22. PMID: 11159882
  4. 克劳丁超家族蛋白在细胞外转运中的作用  |  Heiskala, M., et al. 2001. Traffic. 2: 93-8. PMID: 11247307
  5. 白细胞介素-2 受体 beta 亚基对肠上皮紧密连接的依赖性和非依赖性调控。  |  Nishiyama, R., et al. 2001. J Biol Chem. 276: 35571-80. PMID: 11466322
  6. 牛分枝杆菌和非分枝杆菌肉芽肿中S-100α阳性细胞的免疫组织化学分布。  |  Greene, C., et al. 2019. Fluids Barriers CNS. 16: 3. PMID: 30691500
  7. 妊娠早期蜕膜和宫颈腺体中的S-100蛋白。  |  Kakogiannos, N., et al. 2020. Circ Res. 127: 1056-1073. PMID: 32673519
  8. 自噬通过调节 CLDN5(claudin 5)减轻缺氧诱导的血脑屏障损伤  |  Yang, Z., et al. 2021. Autophagy. 17: 3048-3067. PMID: 33280500
  9. 淋巴结中S-100蛋白免疫反应细胞的比较研究。  |  Bilgiç, A., et al. 2023. Nord J Psychiatry. 77: 506-511. PMID: 36662163
  10. 血清中自闭症谱系障碍学龄前儿童中的Claudin-5、Claudin-11、Occludin、Vinculin、Paxillin和β-Catenin水平。  |  Li, Y., et al. 2023. J Nanobiotechnology. 21: 70. PMID: 36855156
  11. 牛输卵管上皮中的S-100蛋白亚基:原位分布和原代细胞培养过程中的变化。  |  Díaz-Coránguez, M., et al. 2023. Cells. 12: PMID: 37830616
  12. S-100 beta beta蛋白在羔羊嗅觉上皮细胞中的免疫细胞化学定位。  |  Sun, ZW., et al. 2024. Brain Behav Immun. 115: 143-156. PMID: 37848095

订购信息

产品名称产品编号规格价格数量收藏夹

claudin-5 抗体 (A-12)

sc-374221
200 µg/ml
$316.00

claudin-5 (A-12): m-IgG Fc BP-HRP 套装

sc-529691
200 µg Ab; 10 µg BP
$354.00

claudin-5 (A-12): m-IgGκ BP-HRP 套装

sc-522664
200 µg Ab, 40 µg BP
$354.00

claudin-5 抗体 (A-12) AC

sc-374221 AC
500 µg/ml, 25% agarose
$416.00

claudin-5 抗体 (A-12) HRP

sc-374221 HRP
200 µg/ml
$316.00

claudin-5 抗体 (A-12) FITC

sc-374221 FITC
200 µg/ml
$330.00

claudin-5 抗体 (A-12) PE

sc-374221 PE
200 µg/ml
$343.00

claudin-5 抗体 (A-12) Alexa Fluor® 488

sc-374221 AF488
200 µg/ml
$357.00

claudin-5 抗体 (A-12) Alexa Fluor® 546

sc-374221 AF546
200 µg/ml
$357.00

claudin-5 抗体 (A-12) Alexa Fluor® 594

sc-374221 AF594
200 µg/ml
$357.00

claudin-5 抗体 (A-12) Alexa Fluor® 647

sc-374221 AF647
200 µg/ml
$357.00

claudin-5 抗体 (A-12) Alexa Fluor® 680

sc-374221 AF680
200 µg/ml
$357.00

claudin-5 抗体 (A-12) Alexa Fluor® 790

sc-374221 AF790
200 µg/ml
$357.00

Hi! Using your antibody for WB in HUVEC extracts isn't working very well... I'm using a dilution of 1:500... And I'm incubating the Ab with the membrane for 48 hours. What can I do in roder to see the clear band?

Asked by: Fawkes
Thank you for your question. We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Please reach out to your local distributor in Mexico or contact Technical Service directly. We are happy to help you optimize and troubleshoot the antibody to make sure you have success with the antibody.
Answered by: Technical Support
Date published: 2018-12-11

does the claudin-5 antibody can be used in formalin fixed paraffin embedded brain tissue?

Asked by: neurozw
Thank you for your question. We have not validated this claudin-5 antibody sc-374221 for use with formalin fixed, paraffin-embedded tissue sections. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Service
Date published: 2017-08-29

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with claudin-5 (A-12): sc-374221 monoclonal antibody?

Asked by: jenni
Thank you for your question We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Santa Cruz TS
Date published: 2017-01-30
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Rated 1 out of 5 by from 浓度有影响一抗稀释1:500-1:1000浓度范围内,WB条带结果一片空白,无任何条带的痕迹;所用marker均完全转移至PVDF膜上且转膜后用丽春红染色,可见明显泳道及蛋白;
Date published: 2022-03-03
Rated 5 out of 5 by from work for wbgot the sample one months ago, and diluted in 1:1000,the band is clean and clear
Date published: 2018-01-18
Rated 4 out of 5 by from Works for WBI got a sample of this antibody earlier and tried it on WB (1:1000). It works alright and the band is clear.
Date published: 2017-10-30
Rated 4 out of 5 by from Great featuresI get it about a month ago, and run recently. The bands were clear, but not only one as showed, I have to pick one at 23kDa for representative.
Date published: 2017-10-30
Rated 5 out of 5 by from Beautiful bandsI have bought this claudin-5 andibody two months ago. It showed high quality band in a low dilution range, very good product, hopes keeping well in the future!!!
Date published: 2017-09-26
Rated 5 out of 5 by from Great!It is perfect in western blotting for human endothelial cell line/ D3 and Rat endothelial cell (RBE4), but not in IF staining.
Date published: 2017-09-21
Rated 3 out of 5 by from Not bad.claudin-5 (A-12): sc-374221. Immunofluorescence staining of PFA-fixed JEG-3 cells showing very weak staining but should be specific. Diluted 1:50. Probably the concentration is still not enough.
Date published: 2017-09-21
Rated 5 out of 5 by from specific/sharp bands detectedThis antibody was tested on human aortic endothelial cells post various treatments. It gives specific band with no background.
Date published: 2017-09-14
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claudin-5 抗体 (A-12) is rated 4.4 out of 5 by 14.
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