Date published: 2025-9-5
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claudin-4 Antibody (A-12) - Western Blotting - Image 387407
claudin-4 抗体 (A-12) HRP: sc-376643 HRP. SW480 (A), MIA PaCa-2 (B) 和 Caco-2 (C) 全细胞裂解液中 claudin-4 表达的 Western 印迹分析.
claudin-4 抗体 (A-12) HRP: sc-376643 HRP. SW480 (A), MIA PaCa-2 (B) 和 Caco-2 (C) 全细胞裂解液中 claudin-4 表达的 Western 印迹分析.

claudin-4 抗体 (A-12): sc-376643

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说明书
  • claudin-4 抗体 A-12 是小鼠单克隆 IgG1 κ，claudin-4抗体, 在30篇文献中引用，规格为200 µg/ml
  • 特异性表位图谱分析在human的claudin-4的C-terminal细胞质域内的183-209氨基酸之间进行
  • claudin-4 抗体 (A-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 claudin-4
  • 抗claudin-4抗体（A-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取claudin-4 (A-12): sc-376643的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 claudin-4 抗体 (A-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与claudin-4 抗体 (A-12) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Claudin 抗体包括 claudin-1, claudin-10, claudin-11, claudin-19, claudin-2, claudin-3, claudin-4, claudin-5, claudin-6 和 claudin-9

claudin-4抗体（A-12）是一种IgG1 κ小鼠单克隆claudin-4抗体（也称为Claudin 4抗体或CLDN4抗体），可通过WB、IP、IF、IHC(P)和ELISA检测到小鼠、大鼠和人类来源的claudin-4蛋白。claudin-4抗体（A-12）既有非偶联的抗claudin-4抗体形式，也有多种偶联形式的抗claudin-4抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。在人类中，Claudin超家族由许多结构相关的蛋白质组成。这些蛋白质是细胞旁转运中紧密连接的重要结构和功能成分。Claudins位于所有紧密连接承载组织的上皮和内皮细胞中。已知三类蛋白质定位于紧密连接，包括Claudins、Occludin和连接粘附分子。Claudins由四个跨膜结构域和两个细胞外环组成，构成紧密连接链。Claudin的表达高度局限于不同组织的特定区域，并可能在通过紧密连接的跨细胞转运中发挥重要作用。Claudin-4在大鼠肝脏中不表达，而在胰腺中，Claudin-4定位于导管上皮的连接处和腺泡细胞的连接处。在大鼠肠道中，Claudin-4的表达高度局限于结肠表面细胞。人类claudin-4基因定位于7q11.23染色体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

claudin-4 抗体 (A-12) 参考文献:

  1. 紧密连接屏障中的跨膜蛋白  |  Fanning, AS., et al. 1999. J Am Soc Nephrol. 10: 1337-45. PMID: 10361874
  2. 产气荚膜梭状芽孢杆菌肠毒素能与紧密连接整体膜蛋白 Claudin-3 的第二个细胞外环结合。  |  Fujita, K., et al. 2000. FEBS Lett. 476: 258-61. PMID: 10913624
  3. 大鼠肝脏, 胰腺和肠道中克劳丁蛋白 2, 3, 4 和 5 的表达和亚细胞定域的异质性。  |  Rahner, C., et al. 2001. Gastroenterology. 120: 411-22. PMID: 11159882
  4. 克劳丁超家族蛋白在细胞外转运中的作用  |  Heiskala, M., et al. 2001. Traffic. 2: 93-8. PMID: 11247307
  5. 白细胞介素-2 受体 beta 亚基对肠上皮紧密连接的依赖性和非依赖性调控。  |  Nishiyama, R., et al. 2001. J Biol Chem. 276: 35571-80. PMID: 11466322
  6. 在人类上皮细胞中通过 p63 对 claudin-4 进行调控。  |  Kojima, T., et al. 2017. Ann N Y Acad Sci. 1405: 25-31. PMID: 28856683
  7. Claudin-4在胃癌发展中的作用。  |  Liu, W. and Li, M. 2020. Scand J Gastroenterol. 55: 1072-1078. PMID: 32715822
  8. Claudin-3和Claudin-4在双侧和单侧乳腺癌中的表达比较。  |  Szade, J., et al. 2021. Neoplasma. 68: 283-289. PMID: 33147053
  9. 尿路上皮癌中的Claudin-4免疫表达。  |  SĂndulescu, AŞ., et al. 2020. Curr Health Sci J. 46: 379-382. PMID: 33717512
  10. Claudin-3和Claudin-4在卵巢癌治疗中的潜在作用。  |  Uthayanan, L. and El-Bahrawy, M. 2022. J Egypt Natl Canc Inst. 34: 24. PMID: 35665865
  11. Claudin-4在卵巢癌中的定位。  |  Neville, MC., et al. 2022. Heliyon. 8: e10862. PMID: 36237976
  12. Claudin-4和D2-40的表达及其在前列腺腺癌中的意义。  |  Kabra, H., et al. 2023. J Cancer Res Ther. 19: S800-S806. PMID: 38384059

订购信息

产品名称产品编号规格价格数量收藏夹

claudin-4 抗体 (A-12)

sc-376643
200 µg/ml
$316.00

claudin-4 (A-12): m-IgGκ BP-HRP 套装

sc-523031
200 µg Ab, 40 µg BP
$354.00

claudin-4 抗体 (A-12) AC

sc-376643 AC
500 µg/ml, 25% agarose
$416.00

claudin-4 抗体 (A-12) HRP

sc-376643 HRP
200 µg/ml
$316.00

claudin-4 抗体 (A-12) FITC

sc-376643 FITC
200 µg/ml
$330.00

claudin-4 抗体 (A-12) PE

sc-376643 PE
200 µg/ml
$343.00

claudin-4 抗体 (A-12) Alexa Fluor® 488

sc-376643 AF488
200 µg/ml
$357.00

claudin-4 抗体 (A-12) Alexa Fluor® 546

sc-376643 AF546
200 µg/ml
$357.00

claudin-4 抗体 (A-12) Alexa Fluor® 594

sc-376643 AF594
200 µg/ml
$357.00

claudin-4 抗体 (A-12) Alexa Fluor® 647

sc-376643 AF647
200 µg/ml
$357.00

claudin-4 抗体 (A-12) Alexa Fluor® 680

sc-376643 AF680
200 µg/ml
$357.00

claudin-4 抗体 (A-12) Alexa Fluor® 790

sc-376643 AF790
200 µg/ml
$357.00

claudin-4 (A-12) 中和勝肽

sc-376643 P
100 µg/0.5 ml
$68.00

I have Anti-claudin-4 Antibody (A-12): sc-376643 AF-647 cojugated. How can i use it for FCM analysis of live cells. Is it possible?

Asked by: GBAYDAS
You can find our recommended flow cytometry protocol under this link: https://www.scbt.com/de/resources/protocols/flow-cytometry However, the epitope of this antibody is a C-terminal sequence within a cytoplasmic domain of claudin-4. This means that any flow cytometry staining will require permeabilization.
Answered by: Tech Support Europe
Date published: 2021-08-31

What is the best method of antigen retrieval for this antibody?

Asked by: scta1102
Thank you for your question. We recommend using heat treatment with sodium citrate buffer, pH 6.0. A complete protocol for this preferred method of antigen retrieval is available on our website.
Answered by: Technical Support
Date published: 2019-03-11

What is the recommended antigen retrieval method used with this claudin-4 antibody in formalin-fixed paraffin-embedded tissue sections?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2017-01-27
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Rated 2 out of 5 by from 腹水セルブロックで染色しました。Leica Bond maxで50倍希釈、PAb15分、pH6,9のそれぞれで20分で賦活してます。癌腫症例だったのですが、染色強度が弱く感じ、染まる細胞と染まらない細胞がありました。他のレビューが良かったので、条件の設定が悪かったかもしれませんが、自施設では上記としました。
Date published: 2021-09-28
Rated 5 out of 5 by from Excellent antibody for western blotThis antibody performed nicely for Western blot using mouse cell lysates.
Date published: 2019-08-05
Rated 5 out of 5 by from Sehr gut im Western-BlotAntikörper hat sehr gut im Westernblot mit NCI-H292 funktioniert.
Date published: 2018-09-18
Rated 4 out of 5 by from 免疫染色に有用最近購入しましたが、組織の免疫染色にとても有効です。きれいに染まります。細胞の免疫染色はまだ試していませんが、価値ありそうです。
Date published: 2018-08-23
Rated 4 out of 5 by from Good antibodyThis antibody works well on frozen tissue sections and immunofluorescence. It did not worked in western blot.
Date published: 2017-10-17
Rated 4 out of 5 by from Works OK.claudin-4 (A-12): sc-376643. Immunofluorescence staining of PFA-fixed JEG-3 cells showing weak cytoplasmic staining and scattered localization. Diluted 1:50. Thank you for the free sample.
Date published: 2017-09-21
Rated 5 out of 5 by from Good production to test bands in western blotTwo months ago, I used claudin-4 antibody to test the corresponding protein ，and the results is perfect. later I had tried there times acquiring the same results.
Date published: 2017-09-10
Rated 5 out of 5 by from excellent antibodyI bought this two weeks ago and it worked nicely in mouse colon epithelial cells.
Date published: 2017-05-17
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claudin-4抗体 (A-12) is rated 4.4 out of 5 by 10.
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