Citric Acid Monohydrate CAS: 5949-29-1
MF: C6H8O7•H2O
MW: 210.14
A useful buffer component for antigen and epitope unmasking.

Citric Acid Monohydrate (CAS 5949-29-1)

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同义词: 2-Hydroxy-1,2,3-propanetricarboxylic acid monohydrate
应用; A useful buffer component for antigen and epitope unmasking
CAS号码: 5949-29-1
纯度: ≥98%
分子量: 210.14
分子式: C6H8O7•H2O
* 参考分析证明 大量特定数据 (包括水 含量).
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Citric Acid Monohydrate is an acidic compound from citrus fruits; as a starting point in the Krebs cycle, citrate is a key intermediate in metabolism. Citric acid is one of a series of compounds responsible for the physiological oxidation of fats, carbohydrates, and proteins to carbon dioxide and water. It has been used to prepare citrate buffer for antigen retrieval of tissue samples. The citrate solution is designed to break protein cross-links, thus unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, and resulting in enhanced staining intensity of antibodies. Citrate has anticoagulant activity; as a calcium chelator, it forms complexes that disrupt the tendency of blood to clot. May be used to adjust pH and as a sequestering agent for the removal of trace metals.
Additional forms available:
Citric Acid, Anhydrous (sc-211113)
Sodium Citrate, Dihydrate (sc-203383)
Citric Acid Trisodium Salt (sc-214745)
Sodium citrate monobasic (sc-215869)
Sodium citrate tribasic hydrate (sc-236898)
Citrate Concentrated Solution (sc-294091)


参考文献

1. Vandemark, P.J. and Smith, P.F. 1964. J. Bacteriol. 88: 1602-1607. PMID: 14240945
2. Shi, S.R., et al. 1993. J. Histochem. Cytochem. 41: 1599-1604. PMID: 7691930
3. Echezarreta, G., et al. 1997. Sangre. 42: 369-375. PMID: 9424736
4. Uszyński, W., et al. 2007. Folia Histochem. Cytobiol. 45: 33-36. PMID: 17378243

物理状态 :
Solid
溶解度 :
Soluble in water (59.2 g/100 ml) at 20° C, ethanol, ether, and water (84.0 g/100 ml) at 100° C.
保存 :
Desiccate at room temperature
密度 :
1.54 g/cm3 at 20° C
折射率 :
n20D 1.36 (lit.)
pK值 :
pKa: pK1: 3.13, pK2: 4.76, pK3: 6.4
仅供科研使用。不可用于诊断或治疗。
德国水公害等级 :
1
RTECS :
GE7810000
PubChem CID :
22230
默克索引 :
14: 2326
MDL 号码 :
MFCD00149972
EC号码 :
201-069-1
Beilstein 注册 :
4018641
SMILES :
C(C(=O)O)C(CC(=O)O)(C(=O)O)O.O

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PMID: # 10873093  Vakkala, M. et al. 2000. Clin. Cancer Res. 6: 2408-2416.

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