Date published: 2026-10-22
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Chk2 Antibody (A-11) - Western Blotting - Image 353236
Chk2 抗体 (A-11): sc-17747. CCRF-CEM (A), PC-12 (B), NIH/3T3 (C) 和 WEHI-231 (D) 全细胞裂解液中 Chk2 表达的 Western 印迹分析.
Chk2 抗体 (A-11): sc-17747. CCRF-CEM (A), PC-12 (B), NIH/3T3 (C) 和 WEHI-231 (D) 全细胞裂解液中 Chk2 表达的 Western 印迹分析.

Chk2 抗体 (A-11): sc-17747

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寫評論提問
说明书
  • Chk2 抗体 A-11 是小鼠单克隆 IgG1 κ，Chk2抗体, 在49篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Chk2的氨基酸1-300
  • Chk2 抗体 (A-11) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Chk2
  • 抗Chk2抗体（A-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是Chk2 Antibody (A-11) for WB applications. 的首选辅助检测试剂，这些试剂现与Chk2 Antibody (A-11) 打包提供（请参阅下面的订购信息）。
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関連項目

额外Chk抗体，包括Chk2

Chk2抗体（A-11）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的Chk2。Chk2单克隆抗体（A-11）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。Chk2在细胞对DNA损伤的反应中起着关键作用，是G2 DNA损伤检查点中的关键调节因子。通过磷酸化Cdc25C来应对DNA损伤，Chk2抑制这种磷酸酶的活性，有效阻止有丝分裂的进行，直到损伤修复。这种调节机制对于维持基因组稳定性至关重要，因为阻止DNA受损的细胞进入有丝分裂，可以降低突变传播的风险。Chk2与其他蛋白质（如Cdc25C）的相互作用凸显了其在细胞周期调控和DNA损伤反应的复杂网络中的重要性，使Chk2 (A-11)抗体成为研究人员研究这些关键细胞过程的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Chk2 抗体 (A-11) 参考文献:

  1. DNA损伤信号传导和癌症中的ATM-Chk2和ATR-Chk1通路。  |  Smith, J., et al. 2010. Adv Cancer Res. 108: 73-112. PMID: 21034966
  2. DNA损伤反应中的CHK2激酶及其他。  |  Zannini, L., et al. 2014. J Mol Cell Biol. 6: 442-57. PMID: 25404613
  3. CHK2-FOXK轴促进自噬程序的转录调控。  |  Chen, Y., et al. 2020. Sci Adv. 6: eaax5819. PMID: 31911943
  4. ATM-CHK2-Beclin 1 轴促进自噬，以维持氧化应激下的 ROS 稳态。  |  Guo, QQ., et al. 2020. EMBO J. 39: e103111. PMID: 32187724
  5. 葡萄糖生成酶 PCK1 缺乏会促进 CHK2 O-GlcNAcylation 和肝细胞癌在葡萄糖缺乏时的生长。  |  Xiang, J., et al. 2021. J Clin Invest. 131: PMID: 33690219
  6. CHK2 通过 ULK1 磷酸化促进代谢应激诱导的自噬  |  Guo, R., et al. 2022. Antioxidants (Basel). 11: PMID: 35740063
  7. ATM-CHK2-TRIM32轴调节ATG7泛素化以启动氧化应激下的自噬。  |  Liu, J., et al. 2023. Cell Rep. 42: 113402. PMID: 37943659
  8. ROS-ATM-Chk2轴介导的代谢和细胞周期重编程在M1巨噬细胞极化中发挥的新作用。  |  Li, C., et al. 2024. Redox Biol. 70: 103059. PMID: 38316066
  9. Chk2通过氧化应激、DNA损伤和p53/TGFβ1诱导的上皮-间充质转化调节Bmi1缺陷引起的肾衰老和纤维化。  |  Lu, J., et al. 2024. Int J Biol Sci. 20: 2008-2026. PMID: 38617548
  10. CHK2-USP7轴的磷酸化-去泛素化正反馈回路在氧化应激下稳定p53。  |  Liu, J., et al. 2024. Cell Rep. 43: 114366. PMID: 38879877

订购信息

产品名称产品编号规格价格数量收藏夹

Chk2 抗体 (A-11)

sc-17747
200 µg/ml
$322.00

Chk2 (A-11): m-IgG Fc BP-HRP 套装

sc-526568
200 µg Ab; 10 µg BP
$361.00

Chk2 (A-11): m-IgG1 BP-HRP 套装

sc-531941
200 µg Ab; 20 µg BP
$361.00

Chk2 抗体 (A-11) AC

sc-17747 AC
500 µg/ml, 25% agarose
$424.00

Chk2 抗体 (A-11) HRP

sc-17747 HRP
200 µg/ml
$322.00

Chk2 抗体 (A-11) FITC

sc-17747 FITC
200 µg/ml
$336.00

Chk2 抗体 (A-11) PE

sc-17747 PE
200 µg/ml
$349.00

Chk2 抗体 (A-11) Alexa Fluor® 488

sc-17747 AF488
200 µg/ml
$364.00

Chk2 抗体 (A-11) Alexa Fluor® 546

sc-17747 AF546
200 µg/ml
$364.00

Chk2 抗体 (A-11) Alexa Fluor® 594

sc-17747 AF594
200 µg/ml
$364.00

Chk2 抗体 (A-11) Alexa Fluor® 647

sc-17747 AF647
200 µg/ml
$364.00

Chk2 抗体 (A-11) Alexa Fluor® 680

sc-17747 AF680
200 µg/ml
$364.00

Chk2 抗体 (A-11) Alexa Fluor® 790

sc-17747 AF790
200 µg/ml
$364.00

Chk2 抗体 (A-11) TRITC

sc-17747 TRITC
200 µg/ml
$326.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Chk2 (A-11): sc-17747 monoclonal antibody?

Asked by: jenniferc
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2017-01-22
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Rated 2 out of 5 by toronto23 from tested by WB on challenged mouse colon extractsWe tested this antibody, after inducing DNA damage, on mouse colon protein extracts. Unfortunately, we got no signal even at a 1/200 dilution, whereas DNA damage was positively checked.
Date published: 2017-10-27
Rated 1 out of 5 by lucha from this antibody can not workwe use this anbibody in our DNA damage experiments,but it can not work in WB,our human samples.
Date published: 2017-09-13
Rated 4 out of 5 by Xiong Lab from Western Blot. The background is a little strong.1:1000 in 5% non-fat milk in PBST. Goat anti-mouse 1:5000 . The background is a little strong.
Date published: 2017-07-05
Rated 1 out of 5 by Anjie from DOES NOT WORK WELL IN OUR SAMPLESBought this antibody last month and it does not work well in our samples.
Date published: 2017-05-09
Rated 5 out of 5 by Satsat from Great features!I have used it before. It worked well for human and mouse cell in WB.
Date published: 2017-04-17
Rated 4 out of 5 by Hollydong from Great feature!I received this antibody a month ago and it worked nicely for immunoblotting.
Date published: 2017-04-01
Rated 4 out of 5 by njau stemcell lab from good WB signalthis antibody is good for WB if ASSAY,and signal is strong
Date published: 2017-03-03
Rated 5 out of 5 by Bill28 from Great WB and IF dataVery nice western blot data; also great results for IHC staining
Date published: 2017-01-31
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Chk2 抗体 (A-11) is rated 4.1 out of 5 by 16.
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