CHD6抗体 (E-6) 推荐用于 mouse, rat 和 human 来源的CHD6 WB, IP, IF 和 ELISA检测

CHD6 抗体 (E-6): sc-393445

CHD6抗体 (E-6) is rated 3.0 out of 5 by 2.
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说明书

    • 抗-CHD6 抗体 E-6 是小鼠单克隆 IgG2a (kappa轻链),CHD6抗体,规格为200 µg/ml
    • 免疫原氨基酸序列243-320位于human物种的CHD6的N-terminus附近
    • 抗-CHD6 抗体 (E-6) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 CHD6
    • 抗-CHD6 抗体 (E-6) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 可用于ChIP的TransCruz试剂(sc-393445 X, 200 µg/0.1 ml)
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取CHD6 (E-6): sc-393445免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

What blocking solution should I use with CHD6 (E-6): sc-393445 monoclonal antibody for Western blot?

Asked by: Germaine
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
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Rated 1 out of 5 by from sc-393445 does not work with IFs We tried the sc-393445 anti-CHD6 in immunofluorescent antibodies and we could not detect it under the microscope. Used on crosslinked HUVECs with 4% paraformaldehyde
Date published: 2017-12-18
Rated 5 out of 5 by from Very good Western blot data of CHD6 expression Very good Western blot data of CHD6 expression in HeLa and Y79 whole cell lysates. -SCBT QC
Date published: 2013-10-01
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