Date published: 2025-9-6
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Cdk4 Antibody (DCS-35) - Western Blotting - Image 391190
Cdk4 抗体 (DCS-35) Alexa Fluor® 488: sc-23896 AF488. HeLa (A) 和 F9 (B) 全细胞裂解液中 Cdk4 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 用 Cruz Marker MW Tag-Alexa Fluor® 680 检测 Cruz Marker™ 分子量标准: sc-516730.
Cdk4 抗体 (DCS-35) Alexa Fluor® 488: sc-23896 AF488. HeLa (A) 和 F9 (B) 全细胞裂解液中 Cdk4 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 用 Cruz Marker MW Tag-Alexa Fluor® 680 检测 Cruz Marker™ 分子量标准: sc-516730.

Cdk4 抗体 (DCS-35): sc-23896

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寫評論提問

说明书
  • Cdk4 抗体 DCS-35 是小鼠单克隆 IgG1 κ，Cdk4抗体, 在208篇文献中引用，规格为200 µg/ml
  • 与全长重组human Cdk4结合，表位定位在氨基酸1-20上
  • Cdk4 抗体 (DCS-35) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Cdk4
  • 抗Cdk4抗体（DCS-35）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cdk4 (DCS-35): sc-23896的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Cdk4 抗体 (DCS-35) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cdk4 抗体 (DCS-35) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Cdk 抗体 包括 Cdk1, Cdk11, Cdk2, Cdk3, Cdk4, Cdk5, Cdk6, Cdk7, Cdk8和Cdk9

Cdk4抗体（DCS-35）是一种小鼠单克隆IgG1 kappa轻链抗体，用于检测小鼠、大鼠和人类体内的细胞周期蛋白依赖性激酶4（Cdk4），也称为CDC2样激酶4。抗Cdk4抗体（DCS -35）针对全长重组人Cdk4蛋白前20个氨基酸内的表位，确保在应用中（如：蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA））的有效识别。Cdk4是细胞周期依赖性激酶家族的重要成员，通过与D型细胞周期蛋白结合，在G1期控制细胞生长，从而在调节细胞周期进展中发挥关键作用。Cdk4的功能对于驱动细胞周期前进的关键底物的磷酸化至关重要，因此Cdk4成为癌症研究和细胞增殖研究的重要焦点。Cdk4的活性通过以下两种方式调节：与增强激酶活性的细胞周期蛋白相互作用，以及与抑制其功能的抑制剂（如p16）相互作用。此外，Cdk4-细胞周期蛋白复合物的激活还涉及Cdk激活蛋白（CAK）的磷酸化，这凸显了细胞周期进展的复杂调节机制。Cdk4 (DCS-35) 单克隆抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Cdk4 (DCS-35)抗体能够可靠地检测多种物种和应用的Cdk4，是研究人员研究细胞周期动力学和相关信号通路的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdk4 抗体 (DCS-35) 参考文献:

  1. 新型 cdc2/CDC28 相关蛋白激酶基因家族的两个成员 PCTAIRE-1 和 PCTAIRE-3。  |  Okuda, T., et al. 1992. Oncogene. 7: 2249-58. PMID: 1437147
  2. 不同的 CDK6 复合物决定了肿瘤细胞对 CDK4/6 抑制剂和降解剂的反应。  |  Wu, X., et al. 2021. Nat Cancer. 2: 429-443. PMID: 34568836
  3. 过度表达的Cyclin D1和CDK4蛋白是乳腺癌对CDK4/6抑制剂产生抗药性的原因，而PI3K/mTOR抑制剂可以逆转这种抗药性。  |  Cai, Z., et al. 2023. Sci China Life Sci. 66: 94-109. PMID: 35982377
  4. CDK4：细胞周期的主要调节因子及其在癌症中的作用。  |  Baker, SJ., et al. 2022. Genes Cancer. 13: 21-45. PMID: 36051751
  5. 细胞周期激酶  |  Pines, J. 1994. Semin Cancer Biol. 5: 305-13. PMID: 7803767
  6. 小鼠 MO15 相关激酶对细胞周期蛋白依赖性激酶 4 (cdk4) 的激活作用  |  Matsuoka, M., et al. 1994. Mol Cell Biol. 14: 7265-75. PMID: 7935441
  7. cdk4 激活激酶对细胞周期蛋白 D 依赖性激酶 4 (cdk4) 的调控。  |  Kato, JY., et al. 1994. Mol Cell Biol. 14: 2713-21. PMID: 8139570
  8. 细胞周期控制中的一种新的调节基因，可对细胞周期蛋白 D/CDK4 产生特异性抑制作用。  |  Serrano, M., et al. 1993. Nature. 366: 704-7. PMID: 8259215
  9. 细胞周期蛋白, 细胞周期蛋白依赖性激酶和 cdk 抑制剂：对细胞周期控制和癌症的影响。  |  MacLachlan, TK., et al. 1995. Crit Rev Eukaryot Gene Expr. 5: 127-56. PMID: 8845581
  10. 细胞周期蛋白依赖性激酶 4 和 6 抑制剂基因家族 p15, p16, p18 和 p19 在白血病和淋巴瘤中的作用。  |  Siebert, R., et al. 1996. Leuk Lymphoma. 23: 505-20. PMID: 9031081
  11. 神经肿瘤学的最新概念：细胞周期--综述。  |  Dirks, PB. and Rutka, JT. 1997. Neurosurgery. 40: 1000-13; discussion 1013-5. PMID: 9149259
  12. 鉴定参与细胞周期蛋白 D1 和 p16 结合的 CDK4 序列。  |  Coleman, KG., et al. 1997. J Biol Chem. 272: 18869-74. PMID: 9228064

订购信息

产品名称产品编号规格价格数量收藏夹

Cdk4 抗体 (DCS-35)

sc-23896
200 µg/ml
$316.00

Cdk4 (DCS-35): m-IgG Fc BP-HRP 套装

sc-528380
200 µg Ab; 10 µg BP
$354.00

Cdk4 (DCS-35): m-IgGκ BP-HRP 套装

sc-520744
200 µg Ab, 40 µg BP
$354.00

Cdk4 (DCS-35): m-IgG1 BP-HRP 套装

sc-542907
200 µg Ab; 20 µg BP
$354.00

Cdk4 抗体 (DCS-35) AC

sc-23896 AC
500 µg/ml, 25% agarose
$416.00

Cdk4 抗体 (DCS-35) HRP

sc-23896 HRP
200 µg/ml
$316.00

Cdk4 抗体 (DCS-35) FITC

sc-23896 FITC
200 µg/ml
$330.00

Cdk4 抗体 (DCS-35) PE

sc-23896 PE
200 µg/ml
$343.00

Cdk4 抗体 (DCS-35) Alexa Fluor® 488

sc-23896 AF488
200 µg/ml
$357.00

Cdk4 抗体 (DCS-35) Alexa Fluor® 546

sc-23896 AF546
200 µg/ml
$357.00

Cdk4 抗体 (DCS-35) Alexa Fluor® 594

sc-23896 AF594
200 µg/ml
$357.00

Cdk4 抗体 (DCS-35) Alexa Fluor® 647

sc-23896 AF647
200 µg/ml
$357.00

Cdk4 抗体 (DCS-35) Alexa Fluor® 680

sc-23896 AF680
200 µg/ml
$357.00

Cdk4 抗体 (DCS-35) Alexa Fluor® 790

sc-23896 AF790
200 µg/ml
$357.00

Hi, what is the specific epitope for this antibody please (i.e the specific residues on Cdk4)?Thanks.

Asked by: fs605
Thank you for your question. Cdk4 Antibody (DCS-35): sc-23896 was raised against full length recombinant human Cdk4, with epitope mapping to amino acids 1-20.
Answered by: Tech Support Europe
Date published: 2025-04-23

Hi. I have mouse sample and would like to do WB, using this Cdk4 Antibody (sc-23896) as my primary antibody. Can you please suggest a suitable secondary antibody? Or do you have other recommendation?

Asked by: faza
Thank you for your question. We recommend m-IgGκ BP-HRP: sc-516102 as a secondary antibody for this product. We also offer secondary binding proteins with other conjugation options, you can find them under this link: https://www.scbt.com/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Tech Support Europe
Date published: 2020-08-11

Can you please send me a free sample of this Ab to validate its IF staining in my cells? Thank you!

Asked by: Afifim
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-23896 PE), fluorescein (sc-23896 FITC), Alexa Fluor® 488 (sc-23896 AF488) or Alexa Fluor® 647 (sc-23896 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-30
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Rated 5 out of 5 by from Great for WBIt is a good antibody for western blot. I'd recommend it.
Date published: 2024-03-19
Rated 3 out of 5 by from BeneIn westerb blot dà alcuni segnali aspecifici a varie altezze ma nel complesso funziona bene.
Date published: 2023-11-30
Rated 3 out of 5 by from Good antibodyUsed on melanoma cell lines both wild type and BRAF mutated, but to obtain a defined band i had to load an high amount of proteins and i had to use the antibody at a concentration of 1:200. I used the li-cor system for the detection
Date published: 2022-07-19
Rated 5 out of 5 by from Western BlotWe used it 1:500 dilution in 5%milk - TBST. Intense bands at the predicted molecular weight.
Date published: 2022-05-23
Rated 5 out of 5 by from 出色好用就是太贵了，能送点我试用装就更好了，东西一流，服务一流，物流一流，一流的公司真不错，毕业了去这个公司面试
Date published: 2022-01-05
Rated 1 out of 5 by from poor staining in immunohistochemical studyCDK4 Antibody (DSC-35): immunoperoxidase staining of formalin fixed, paraffin-embedded human dedifferentiated liposarcoma tissue showing poor nuclear staining of tumor cells.
Date published: 2021-07-14
Rated 4 out of 5 by from suitable for WBThis antibody is suitable for WB (1:1000) in human cell lines
Date published: 2019-10-03
Rated 1 out of 5 by from Very low signalI tested the antibody in a few cell lines that I know express CDK4 (detected in WB by other antibodies). The signal for CDK4 was barely detectable after a long exposure with sensitive ECL. The customer service at Santa Cruz was great since they offered this as a replacement for another antibody that didn't work either but I had no results with two different antibodies.
Date published: 2018-08-08
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Cdk4 抗体 (DCS-35) is rated 4.1 out of 5 by 34.
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