Date published: 2025-9-6

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Cdk2 抗体 (D-12): sc-6248

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说明书
  • Cdk2抗体(D-12)是小鼠单克隆IgG1 κ, 在494篇文献中引用,规格为200 µg/ml
  • 免疫1-298氨基酸,对应于human来源Cdk2全长
  • 抗-Cdk2 抗体 (D-12) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Cdk2
  • 抗Cdk2抗体(D-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 关于如何获取Cdk2 (D-12): sc-6248免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Cdk2 Antibody (D-12) for WB applications. 的首选辅助检测试剂,这些试剂现与Cdk2 Antibody (D-12) 打包提供(请参阅下面的订购信息)。

快捷链接

関連項目

Cdk2 抗体(D-12)是一种小鼠单克隆 IgG1 kappa 轻链抗体,专门针对细胞周期依赖性激酶 2(Cdk2)。Cdk2单克隆抗体(D-12)针对氨基酸1-298(代表全长人类Cdk2蛋白)进行免疫,可有效识别小鼠、大鼠和人类物种的Cdk2,适用于Western Blot(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附(ELISA)等应用。Cdk2通过与周期蛋白E和A形成复合物,在细胞周期调节中发挥关键作用,而周期蛋白E和A对于从G1期到S期的过渡以及S期的进展至关重要。这种激酶的活性可确保DNA复制和细胞分裂正常进行,因此Cdk2成为研究细胞增殖和癌症机制的重要靶标。Cdk2单克隆抗体(D-12)有非结合型和多种结合型,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®结合型,可满足不同实验需求。Cdk2 (D-12)抗体可促进Cdk2的检测和分析,支持细胞周期动力学、癌症生物学以及针对细胞增殖的治疗干预措施的研发。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdk2 抗体 (D-12) 参考文献:

  1. 人类细胞周期的两个阶段都需要 Cyclin A。  |  Pagano, M., et al. 1992. EMBO J. 11: 961-71. PMID: 1312467
  2. CDK2 编码一种 33 kDa 的细胞周期蛋白 A 相关蛋白激酶,在细胞周期中比 CDC2 表达得更早。  |  Elledge, SJ., et al. 1992. Proc Natl Acad Sci U S A. 89: 2907-11. PMID: 1372993
  3. 新型单克隆抗体鉴定出人类 CDC2 的不同形式。  |  Lukás, J., et al. 1992. Eur J Biochem. 207: 169-76. PMID: 1628647
  4. 人类 D 型细胞周期蛋白  |  Xiong, Y., et al. 1991. Cell. 65: 691-9. PMID: 1827756
  5. 爪蟾卵母细胞成熟过程中的环蛋白及其伙伴  |  Kobayashi, H., et al. 1991. Cold Spring Harb Symp Quant Biol. 56: 437-47. PMID: 1840257
  6. cdc2 的可逆酪氨酸磷酸化:进入有丝分裂过程中伴随着去磷酸化激活。  |  Morla, AO., et al. 1989. Cell. 58: 193-203. PMID: 2473839
  7. cdc2 激酶是一种核蛋白,对哺乳动物细胞的有丝分裂至关重要。  |  Riabowol, K., et al. 1989. Cell. 57: 393-401. PMID: 2541912
  8. 人细胞周期蛋白 cDNA 的分离:细胞周期蛋白 mRNA 和蛋白质在细胞周期中的调控以及与 p34cdc2 相互作用的证据。  |  Pines, J. and Hunter, T. 1989. Cell. 58: 833-46. PMID: 2570636
  9. Cip1 通过抑制依赖细胞周期蛋白的激酶,阻止了爪蟾提取物中 DNA 复制的启动。  |  Strausfeld, UP., et al. 1994. Curr Biol. 4: 876-83. PMID: 7850420
  10. 氮芥诱导的人体细胞G2延迟对细胞周期蛋白A/cdk2和细胞周期蛋白B1/cdc2-激酶复合物的影响不同。  |  O'Connor, PM., et al. 1993. J Biol Chem. 268: 8298-308. PMID: 8463339
  11. CDK2和cdc2激酶及其共同伙伴细胞周期蛋白A在甲状腺上皮细胞中的表达和亚细胞定位:依赖环磷酸腺苷和不依赖环磷酸腺苷的细胞周期的比较。  |  Baptist, M., et al. 1996. J Cell Physiol. 166: 256-73. PMID: 8591985
  12. 在爪蟾卵提取物中,细胞周期蛋白 A 和细胞周期蛋白 E 都具有促进 S 期(SPF)的活性。  |  Strausfeld, UP., et al. 1996. J Cell Sci. 109 (Pt 6): 1555-63. PMID: 8799842

订购信息

产品名称产品编号规格价格数量收藏夹

Cdk2 抗体 (D-12)

sc-6248
200 µg/ml
$316.00

Cdk2 (D-12): m-IgG Fc BP-HRP 套装

sc-526484
200 µg Ab; 10 µg BP
$354.00

Cdk2 (D-12): m-IgG1 BP-HRP 套装

sc-531857
200 µg Ab; 20 µg BP
$354.00

Cdk2 抗体 (D-12) AC

sc-6248 AC
500 µg/ml, 25% agarose
$416.00

Cdk2 抗体 (D-12) HRP

sc-6248 HRP
200 µg/ml
$316.00

Cdk2 抗体 (D-12) FITC

sc-6248 FITC
200 µg/ml
$330.00

Cdk2 抗体 (D-12) PE

sc-6248 PE
200 µg/ml
$343.00

Cdk2 抗体 (D-12) Alexa Fluor® 488

sc-6248 AF488
200 µg/ml
$357.00

Cdk2 抗体 (D-12) Alexa Fluor® 546

sc-6248 AF546
200 µg/ml
$357.00

Cdk2 抗体 (D-12) Alexa Fluor® 594

sc-6248 AF594
200 µg/ml
$357.00

Cdk2 抗体 (D-12) Alexa Fluor® 647

sc-6248 AF647
200 µg/ml
$357.00

Cdk2 抗体 (D-12) Alexa Fluor® 680

sc-6248 AF680
200 µg/ml
$357.00

Cdk2 抗体 (D-12) Alexa Fluor® 790

sc-6248 AF790
200 µg/ml
$357.00

Cdk2 抗体 (D-12) TRITC

sc-6248 TRITC
200 µg/ml
$320.00

Cdk2 抗体 (D-12) Alexa Fluor® 405

sc-6248 AF405
200 µg/ml
$357.00

is this antibody available as a biotin conjugate?

Asked by: George
Thank you for your question. To request a price quote and ship date for the biotin conjugate please email specialorders@scbt.com.
Answered by: BlakeJ
Date published: 2024-11-05

What is the recommended secondary reagent to be used with this Cdk (D-12) mouse monoclonal primary by Western blot?

Asked by: jenni
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Santa Cruz TS
Date published: 2016-12-20

Can I use Cdk2 Antibody (D-12) mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: jenni
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. Cdk2 (D-12) mouse monoclonal antibody is currently available conjugated to HRP, FITC, TRITC, PE, AF405, AF488 and AF647
Answered by: Santa Cruz TS
Date published: 2016-12-20
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Rated 5 out of 5 by from Western blotGood performance in western blot. Buffer 5% milk - TBST, intense bands at the predicted molecular weight
Date published: 2022-05-23
Rated 1 out of 5 by from 抗体非特异性不好WB目的条带上有杂带,而且IF非特异性信号特别多,无法区分WT和Cdk2-KO,根本不像展示的那么好!
Date published: 2019-11-23
Rated 5 out of 5 by from good1/500 dilution was used for WB, and the results are good.
Date published: 2018-12-24
Rated 5 out of 5 by from Great product!!It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 5 out of 5 by from Great features!I bought this a month ago and am so happy that I had gotten a perfect picture of Western blot analysis.
Date published: 2017-12-12
Rated 5 out of 5 by from Great features!I bought this a month ago and am so happy that I had gotten a perfect picture of Western blot analysis.
Date published: 2017-12-12
Rated 5 out of 5 by from Great antibody for IB!This antibody is great for IB, same result compared to CST #2546, but much cheaper.
Date published: 2017-11-28
Rated 5 out of 5 by from Great!the antibody is good at several human lung cancer cell line at a dilution of 1:1000.
Date published: 2017-11-07
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Cdk2 抗体 (D-12) is rated 4.8 out of 5 by 44.
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