Date published: 2026-10-22
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Cdc6 Antibody (D-1) - Western Blotting - Image 135722
未经处理 (A, D), 血清饥饿和血清处理 (B, E) 以及血清饥饿, 血清处理和λ蛋白磷酸酶 (sc-200312A) 处理 (C, F) 的 HeLa 全细胞裂解液中 Cdc6 磷酸化的 Western 印迹分析. 测试的抗体包括 p-Cdc6 (Ser 54)-R: sc-12920-R (A, B, C) 和 Cdc6 (D-1): sc-13136 (D, E, F).
未经处理 (A, D), 血清饥饿和血清处理 (B, E) 以及血清饥饿, 血清处理和λ蛋白磷酸酶 (sc-200312A) 处理 (C, F) 的 HeLa 全细胞裂解液中 Cdc6 磷酸化的 Western 印迹分析. 测试的抗体包括 p-Cdc6 (Ser 54)-R: sc-12920-R (A, B, C) 和 Cdc6 (D-1): sc-13136 (D, E, F).

Cdc6 抗体 (D-1): sc-13136

4.3(9)
寫評論提問
说明书
  • Cdc6抗体(D-1)是小鼠单克隆IgG1 κ, 在12篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Cdc6的氨基酸257-560
  • Cdc6 抗体 (D-1) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 Cdc6
  • 抗Cdc6抗体（D-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是Cdc6 Antibody (D-1) for WB applications. 的首选辅助检测试剂，这些试剂现与Cdc6 Antibody (D-1) 打包提供（请参阅下面的订购信息）。
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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Cdc抗体，包括Cdc11, Cdc123, Cdc13, Cdc14 Phosphatase, Cdc16, Cdc2, Cdc20B, Cdc25, Cdc26, Cdc27, Cdc28, Cdc34, Cdc37, Cdc4, Cdc40和Cdc42

Cdc6抗体（D-1）是一种鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测人类来源的Cdc6。Cdc6 单克隆抗体（D-1）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。Cdc6 在 DNA 复制的启动中起着至关重要的作用，而 DNA 复制对于细胞分裂和细胞周期的正常进行至关重要。Cdc6通过确保DNA复制在正确的时间进行，帮助维持基因组的稳定性，防止可能导致细胞功能异常或疾病的错误。Cdc6与细胞周期调节中涉及的几种关键蛋白相互作用，包括细胞周期不同阶段之间过渡至关重要的细胞周期蛋白依赖性激酶和各种泛素连接酶。对这些相互作用的调节可确保细胞准确复制DNA并正确分裂，从而支持整体细胞健康和功能。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdc6 抗体 (D-1) 参考文献:

  1. 利用慢病毒 RNA 干扰表达载体下调人 Cdc6 蛋白。  |  Luo, F., et al. 2006. Methods Mol Biol. 342: 287-93. PMID: 16957383
  2. Cdc6：在致癌过程中起关键作用的多功能分子开关。  |  Petrakis, TG., et al. 2012. Transcription. 3: 124-9. PMID: 22771947
  3. ORC/Cdc6/MCM2-7复合物--调节螺旋酶装载的新力量。  |  Riera, A., et al. 2013. Cell Cycle. 12: 2155-6. PMID: 23803736
  4. CDC6 在多形性胶质母细胞瘤中上调并成为不良预后特征。  |  Zhao, H., et al. 2021. Clin Transl Oncol. 23: 565-571. PMID: 32661826
  5. CDC6 可能是肝细胞癌的生物标志物  |  Kong, DG. and Yao, FZ. 2021. Int J Clin Exp Pathol. 14: 811-818. PMID: 34367412
  6. CDC6 是 CDK1 激活动态以及有丝分裂进入和进展时间的关键抑制调节因子。  |  El Dika, M., et al. 2023. Biology (Basel). 12: PMID: 37372141
  7. CDC6 是一种关键的复制许可因子，在弥漫大 B 细胞淋巴瘤中过度表达并导致不良预后。  |  Shen, M., et al. 2023. BMC Cancer. 23: 978. PMID: 37833632
  8. TOP2A和CDC6在肝癌中的作用  |  Jia, W., et al. 2023. Medicine (Baltimore). 102: e35604. PMID: 37861550
  9. 基于 TCGA 和 GEO 数据挖掘和实验分析，CDC6 可作为肺腺癌预后和进展的指标。  |  Lou, H., et al. 2024. Oncol Rep. 51: PMID: 38186304
  10. 通过抑制CDC6和阻断DNA复制，双重抑制SYK和EGFR克服了化疗耐药性。  |  Mandal, J., et al. 2024. Cancer Res. 84: 3881-3893. PMID: 39120597

订购信息

产品名称产品编号规格价格数量收藏夹

Cdc6 抗体 (D-1)

sc-13136
200 µg/ml
$322.00

Cdc6 (D-1): m-IgG Fc BP-HRP 套装

sc-526539
200 µg Ab; 10 µg BP
$361.00

Cdc6 (D-1): m-IgG1 BP-HRP 套装

sc-531912
200 µg Ab; 20 µg BP
$361.00

Cdc6 抗体 (D-1) AC

sc-13136 AC
500 µg/ml, 25% agarose
$424.00

Cdc6 抗体 (D-1) HRP

sc-13136 HRP
200 µg/ml
$322.00

Cdc6 抗体 (D-1) FITC

sc-13136 FITC
200 µg/ml
$336.00

Cdc6 抗体 (D-1) PE

sc-13136 PE
200 µg/ml
$349.00

Cdc6 抗体 (D-1) Alexa Fluor® 488

sc-13136 AF488
200 µg/ml
$364.00

Cdc6 抗体 (D-1) Alexa Fluor® 546

sc-13136 AF546
200 µg/ml
$364.00

Cdc6 抗体 (D-1) Alexa Fluor® 594

sc-13136 AF594
200 µg/ml
$364.00

Cdc6 抗体 (D-1) Alexa Fluor® 647

sc-13136 AF647
200 µg/ml
$364.00

Cdc6 抗体 (D-1) Alexa Fluor® 680

sc-13136 AF680
200 µg/ml
$364.00

Cdc6 抗体 (D-1) Alexa Fluor® 790

sc-13136 AF790
200 µg/ml
$364.00

What is the recommended fixation method for immunofluorescence staining with Cdc6 (D-1): sc-13136 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by sjieya from Great antibody for Western blotThis antibody performs very well for Western blot for multiple human cell lines.
Date published: 2020-01-05
Rated 1 out of 5 by zusl from CDC6 AbThe antibody didn´t give me any signal using HCT116 nuclear extracts performed by standard WB procedure that worked for another Santa Cruz antibodies (for example sc-9964 – also against CDC6).
Date published: 2018-07-23
Rated 5 out of 5 by cjMara from Excellent antibody! Published for ChIP.This monoclonal Cdc6 Antibody (D-1) has excellent in-house data for Western blot and IF, and it has been published for ChIP (see PMID: 26872786).
Date published: 2017-02-07
Rated 5 out of 5 by Steffi from good antibodygood antibody, used in WB on human samples
Date published: 2016-07-25
Rated 4 out of 5 by SH from This antibody works well and can be usedThis antibody works well and can be used for IP and Western Blot with human HeLa, T98G, U2-OS, HEK 293T, H1299 cell lines. -SCBT Publication Review
Date published: 2015-04-29
Rated 5 out of 5 by MS from Works well in Western Blot application withWorks well in Western Blot application with the human HEF cell line. -SCBT Publication Review
Date published: 2015-03-18
Rated 5 out of 5 by JL from Strong band at correct molecular weight observedStrong band at correct molecular weight observed by Western Blot with HeLa nuclear extract. -SCBT QC
Date published: 2015-01-19
Rated 5 out of 5 by JM from Produced good quality flow cytometry dataProduced good quality flow cytometry data in HeLa and S3 cells. -SCBT Publication Review
Date published: 2015-01-09
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Cdc6 抗体 (D-1) is rated 4.3 out of 5 by 9.
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