Date published: 2025-9-6
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Cdc37 Antibody (E-4) - Western Blotting - Image 358010
Cdc37 抗体 (E-4): sc-13129. Jurkat (A), K-562 (B), A-431 (C), NIH/3T3 (D), SW480 (E) 和 MCF7 (F) 全细胞裂解液中 Cdc37 表达的 Western 印迹分析.
Cdc37 抗体 (E-4): sc-13129. Jurkat (A), K-562 (B), A-431 (C), NIH/3T3 (D), SW480 (E) 和 MCF7 (F) 全细胞裂解液中 Cdc37 表达的 Western 印迹分析.

Cdc37 抗体 (E-4): sc-13129

4.9(11)
寫評論提問

说明书
  • Cdc37 抗体 E-4 是小鼠单克隆 IgG2b κ，Cdc37抗体, 在39篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Cdc37的氨基酸108-378
  • Cdc37 抗体 (E-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Cdc37
  • 抗Cdc37抗体（E-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 关于如何获取Cdc37 (E-4): sc-13129的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Cdc37 抗体 (E-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cdc37 抗体 (E-4) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Cdc 抗体 包括 Cdc11, Cdc123, Cdc13, Cdc14 Phosphatase, Cdc16, Cdc2, Cdc20B, Cdc25, Cdc26和Cdc27

Cdc37抗体（E-4）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC）和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人类来源的Cdc37。Cdc37 (E-4) 抗体有非结合型和多种结合型可供选择，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。Cdc37通过促进细胞周期从G1期向S期过渡所必需的细胞周期依赖性激酶4（Cdk4）和细胞周期蛋白D1复合物的形成，在细胞周期调控中发挥着关键作用。Cdc37与热休克蛋白90（Hsp90）形成稳定的复合物，对于参与细胞周期调节的各种客户蛋白的稳定性和功能至关重要。Cdc37的正常运作和相关蛋白相互作用对于维持细胞稳态和防止细胞无序增殖至关重要，而细胞无序增殖会导致癌症。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdc37 抗体 (E-4) 参考文献:

  1. 融合激酶由 Cdc37/Hsp90 稳定，并提高 Gli 蛋白水平。  |  Kise, Y., et al. 2006. Biochem Biophys Res Commun. 351: 78-84. PMID: 17054904
  2. Cdc37 在蛋白激酶质量控制中发挥着独特的作用，可保护新生链不被降解，并促进翻译后成熟。  |  Mandal, AK., et al. 2007. J Cell Biol. 176: 319-28. PMID: 17242065
  3. cdc25 是一种特异性酪氨酸磷酸酶，可直接激活 p34cdc2。  |  Gautier, J., et al. 1991. Cell. 67: 197-211. PMID: 1913817
  4. 酵母(Saccharomyces cerevisiae)cdc突变体中人工染色体的有丝分裂传递。  |  Palmer, RE., et al. 1990. Genetics. 125: 763-74. PMID: 2204582
  5. 针对多种疾病中 Hsp90-Cdc37 相互作用的治疗潜力  |  Zhang, X., et al. 2022. Curr Drug Targets. 23: 1023-1038. PMID: 35400341
  6. RAF1-HSP90-CDC37 复合物的结构揭示了 RAF1 调控的基础。  |  García-Alonso, S., et al. 2022. Mol Cell. 82: 3438-3452.e8. PMID: 36055235
  7. CDC37 基因表达对转移性结直肠癌目的基因治疗的预测价值。  |  Arai, H., et al. 2024. Eur J Cancer. 201: 113914. PMID: 38359495
  8. 细胞周期的新转折  |  Barinaga, M. 1995. Science. 269: 631-2. PMID: 7624789
  9. 含有 CDC27 和 CDC16 的 20S 复合物可催化泛素与细胞周期蛋白 B 的有丝分裂特异性连接。  |  King, RW., et al. 1995. Cell. 81: 279-88. PMID: 7736580
  10. 通过酵母互补克隆出 CDC34 细胞周期基因的人类同源物。  |  Plon, SE., et al. 1993. Proc Natl Acad Sci U S A. 90: 10484-8. PMID: 8248134
  11. 哺乳动物的 p50Cdc37 是 Hsp90 的蛋白激酶靶向亚基，能与 Cdk4 结合并使其稳定。  |  Stepanova, L., et al. 1996. Genes Dev. 10: 1491-502. PMID: 8666233
  12. 一种与酵母 Cdc6p 相关的人类蛋白质。  |  Williams, RS., et al. 1997. Proc Natl Acad Sci U S A. 94: 142-7. PMID: 8990175

订购信息

产品名称产品编号规格价格数量收藏夹

Cdc37 抗体 (E-4)

sc-13129
200 µg/ml
$316.00

Cdc37 (E-4): m-IgG Fc BP-HRP 套装

sc-528265
200 µg Ab; 10 µg BP
$354.00

Cdc37 (E-4): m-IgGκ BP-HRP 套装

sc-520600
200 µg Ab, 40 µg BP
$354.00

Cdc37 (E-4): m-IgG2b BP-HRP 套装

sc-550016
200 µg Ab; 10 µg BP
$354.00

Cdc37 抗体 (E-4) AC

sc-13129 AC
500 µg/ml, 25% agarose
$416.00

Cdc37 抗体 (E-4) HRP

sc-13129 HRP
200 µg/ml
$316.00

Cdc37 抗体 (E-4) FITC

sc-13129 FITC
200 µg/ml
$330.00

Cdc37 抗体 (E-4) PE

sc-13129 PE
200 µg/ml
$343.00

Cdc37 抗体 (E-4) Alexa Fluor® 488

sc-13129 AF488
200 µg/ml
$357.00

Cdc37 抗体 (E-4) Alexa Fluor® 546

sc-13129 AF546
200 µg/ml
$357.00

Cdc37 抗体 (E-4) Alexa Fluor® 594

sc-13129 AF594
200 µg/ml
$357.00

Cdc37 抗体 (E-4) Alexa Fluor® 647

sc-13129 AF647
200 µg/ml
$357.00

Cdc37 抗体 (E-4) Alexa Fluor® 680

sc-13129 AF680
200 µg/ml
$357.00

Cdc37 抗体 (E-4) Alexa Fluor® 790

sc-13129 AF790
200 µg/ml
$357.00

Cdc37 抗体 (E-4) TRITC

sc-13129 TRITC
200 µg/ml
$320.00

Hi I would like to use this antibody (Cdc37 (E-4)) for immunoprecipitation. Has anyone tested it for this? How much antibody was used?

Asked by: Henriette
Thank you for your question. This antibody is recommended and warrantied for Immunoprecipitation use, and there are several publications in that application. Here is a link to our recommended protocol: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-08-28

I am using Cdc37 (E-4): sc-13129 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 5
Date published: 2017-01-25
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Rated 5 out of 5 by from Excellent in WBExcellent in WB Dilution 1:1000 clear and specific band
Date published: 2021-02-25
Rated 4 out of 5 by from Great antibodyThis antibody works well for IP and also WB. Thank you, Santa Cruz.
Date published: 2018-03-25
Rated 5 out of 5 by from Good antibodyThis antibody worked very good for WB and IP. WB diluted 1:1000 in 293t cell lysate.
Date published: 2017-09-28
Rated 5 out of 5 by from very helpfulThis antibody is very helpful for analyisis cdc37 in HepG2 cell, I got clear and strong signal band at 50kda.This is the target band, no background by dilution 1;600.
Date published: 2017-08-26
Rated 5 out of 5 by from Pretty goodGood specificity in WB in rat testis tissue, we usually use the dilution 1:300.
Date published: 2017-03-18
Rated 5 out of 5 by from versitile antibodyUsed this antibody for IHC(P), IF and WB in multiple samples, which produced nice and specific signal
Date published: 2017-01-17
Rated 5 out of 5 by from Good cytoplasmic staining of formalin fixedGood cytoplasmic staining of formalin fixed glandular cells. -SCBT Publication Review
Date published: 2015-04-11
Rated 5 out of 5 by from Strong WB band in JurkatStrong WB band in Jurkat, CTLL-2 and KNRK whole cell lysates. -SCBT QC
Date published: 2015-04-11
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Cdc37 抗体 (E-4) is rated 4.9 out of 5 by 11.
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