CD96 CRISPR/Cas9 KO Plasmid (h): sc-407300
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD96 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD96 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD96 Antibody (NK92.50.1): sc-53574
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD96 CRISPR/Cas9 KO Plasmid (h) | sc-407300 | 20 µg | $397.00 |
Overview
CD96 is a type I transmembrane immunoglobulin superfamily receptor expressed primarily on T cells and NK cells, where it modulates immune synapse formation and cytotoxic effector function. It participates in adhesion and co-inhibitory signaling through interactions within the nectin/nectin-like network, influencing signaling pathways that tune activation thresholds and cytokine production. CD96 function intersects with immune checkpoint regulation alongside receptors such as TIGIT and CD226, shaping responses to stressed or transformed cells in the tumor microenvironment. Dysregulated CD96 activity and expression have been associated with altered anti-tumor immunity, chronic inflammation, and immune evasion mechanisms relevant to oncology and immunology research.
CD96 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CD96 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CD96 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CD96 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD96 protein expression.
This CRISPR knockout system enables efficient generation of CD96-deficient cell models for investigation of CD96 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CD96 exon(s) critical for CD96 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CD96 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD96 CRISPR/Cas9 KO Plasmid (h) and CD96 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CD96 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD96 HDR Plasmid (h) and CD96 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CD96 homology arms to support homology-directed repair at defined CD96 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.