CD74 Double Nickase Plasmid (h): sc-400279-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD74 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD74 Double Nickase Plasmid (h) and CD74 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CD74. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD74 Antibody (LN-2): sc-6262
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD74 Double Nickase Plasmid (h) | sc-400279-NIC | 20 µg | $410.00 | |||
CD74 Double Nickase Plasmid (h2) | sc-400279-NIC-2 | 20 µg | $410.00 |
CD74 encodes the invariant chain (Ii), a type II transmembrane protein that functions as an essential chaperone for MHC class II molecules in antigen-presenting cells. By occupying the peptide-binding groove in the endoplasmic reticulum and directing MHC II trafficking through endosomal compartments, CD74 supports peptide loading and presentation to CD4+ T cells as part of adaptive immune signaling. CD74 also serves as a cell-surface receptor component for macrophage migration inhibitory factor (MIF), linking it to downstream pathways such as NF-κB and MAPK that regulate inflammation, cell survival, and immune cell activation. Dysregulated CD74 expression and signaling have been associated with immune-mediated pathology and are frequently studied in the context of hematologic malignancies and inflammatory microenvironments.
CD74 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CD74 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CD74. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CD74 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CD74-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.