CD5 Antibody (CD5/54/F6): sc-53204
- CD5 Antibody (CD5/54/F6) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
- raised against a synthetic peptide corresponding to the intracellular region of CD5 of human origin
- Anti-CD5 Antibody (CD5/54/F6) is recommended for detection of CD5 of mouse, rat, human, rabbit, equine and bovine origin by WB, IP, IF and IHC(P)
- Anti-CD5 Antibody (CD5/54/F6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CD5 Antibody (CD5/54/F6) for WB and IHC(P) applications. These reagents are now offered in bundles with CD5 Antibody (CD5/54/F6) (see ordering information below).
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CD5 Antibody (CD5/54/F6) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD5 protein of mouse, rat, human, rabbit, equine, and bovine origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-CD5 antibody (CD5/54/F6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD5, also known as Lyt-1, is a transmembrane glycoprotein predominantly expressed on approximately 70% of normal peripheral blood lymphocytes and nearly all T lymphocytes in the thymus and peripheral blood. CD5 plays a crucial role in T cell activation; when T cells are activated through the T cell receptor (TCR), CD5 undergoes tyrosine phosphorylation, which is essential for modulating T cell responses. Notably, absence of CD5 leads to hyper-responsiveness of T cells to TCR-mediated activation, highlighting CD5′s importance in maintaining immune homeostasis. CD5 interacts with the TCR/CD3-ζ chain and the Src family kinase Lck p56, significantly enhancing Lck′s kinase activity by 10- to 15-fold when bound to CD5. Additionally, CD5 serves as a receptor for the B cell antigen CD72, although the precise consequences of this interaction remain to be fully elucidated, suggesting a potential role in thymic selection processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD5 Antibody (CD5/54/F6) | sc-53204 | 200 µg/ml | $322.00 | |||
CD5 Antibody (CD5/54/F6): m-IgG Fc BP-HRP Bundle | sc-526778 | 200 µg Ab; 10 µg BP | $361.00 | |||
CD5 Antibody (CD5/54/F6): m-IgG1 BP-HRP Bundle | sc-532151 | 200 µg Ab; 20 µg BP | $361.00 | |||
CD5 Antibody (CD5/54/F6) AC | sc-53204 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CD5 Antibody (CD5/54/F6) HRP | sc-53204 HRP | 200 µg/ml | $322.00 | |||
CD5 Antibody (CD5/54/F6) FITC | sc-53204 FITC | 200 µg/ml | $336.00 | |||
CD5 Antibody (CD5/54/F6) PE | sc-53204 PE | 200 µg/ml | $349.00 | |||
CD5 Antibody (CD5/54/F6) Alexa Fluor® 488 | sc-53204 AF488 | 200 µg/ml | $364.00 | |||
CD5 Antibody (CD5/54/F6) Alexa Fluor® 546 | sc-53204 AF546 | 200 µg/ml | $364.00 | |||
CD5 Antibody (CD5/54/F6) Alexa Fluor® 594 | sc-53204 AF594 | 200 µg/ml | $364.00 | |||
CD5 Antibody (CD5/54/F6) Alexa Fluor® 647 | sc-53204 AF647 | 200 µg/ml | $364.00 | |||
CD5 Antibody (CD5/54/F6) Alexa Fluor® 680 | sc-53204 AF680 | 200 µg/ml | $364.00 | |||
CD5 Antibody (CD5/54/F6) Alexa Fluor® 790 | sc-53204 AF790 | 200 µg/ml | $364.00 |