CD45 Antibody (2B11): sc-20056

CD45 Antibody (2B11) is a mouse monoclonal IgG1 kappa light chain antibody designed to target CD45, also known as Protein Tyrosine Phosphatase Receptor Type C (PTPRC). CD45 is a crucial transmembrane glycoprotein expressed on all nucleated hematopoietic cells, playing an essential role in regulating immune cell signaling and activation. Located on the cell surface, CD45 modulates signal transduction pathways by dephosphorylating key tyrosine residues on Src family kinases such as Lck and Fyn, as well as on ZAP-70 from the Syk family of kinases. These interactions are vital for proper T and B cell receptor signaling, influencing lymphocyte development, activation, and immune responses. CD45 monoclonal antibody (2B11) is raised against isolated neoplastic cells from a T cell lymphoma, ensuring effectiveness in targeting CD45 across various human applications, including western blotting (WB), immunoprecipitation (IP), Proximity Ligation Assay (PAR), and flow cytometry (FCM). Anti-CD45 antibody (2B11) recognizes specific epitopes generated by alternative splicing of the CD45 gene, allowing for the detection of multiple isoforms such as CD45RA, CD45RB, CD45RC, and CD45RO. These isoforms are differentially expressed among distinct immune cell subsets, providing valuable insights into immune cell differentiation and function. CD45 (2B11) antibody facilitates detailed analysis of immune cell populations and their signaling mechanisms, making this reagent an indispensable tool for research and diagnostic applications in immunology and hematology.

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CD45 Antibody (2B11) References:

1. CD45 isoforms in T cell signalling and development.  |  McNeill, L., et al. 2004. Immunol Lett. 92: 125-34. PMID: 15081536
2. The continuum of stem cell transdifferentiation: possibility of hematopoietic stem cell plasticity with concurrent CD45 expression.  |  Udani, VM. 2006. Stem Cells Dev. 15: 1-3. PMID: 16522157
3. Innate immune cell CD45 regulates lymphopenia-induced T cell proliferation.  |  Saunders, AE., et al. 2014. J Immunol. 193: 2831-42. PMID: 25114101
4. CD45 phosphatase is crucial for human and murine acute myeloid leukemia maintenance through its localization in lipid rafts.  |  Saint-Paul, L., et al. 2016. Oncotarget. 7: 64785-64797. PMID: 27579617
5. CD45 tyrosine phosphatase activity and membrane anchoring are required for T-cell antigen receptor signaling.  |  Niklinska, BB., et al. 1994. Mol Cell Biol. 14: 8078-84. PMID: 7526153
6. Antibodies to CD45 and other cell membrane antigens in systemic lupus erythematosus.  |  Winfield, JB., et al. 1994. Springer Semin Immunopathol. 16: 201-10. PMID: 7716705
7. CD45 isoform expression on human haemopoietic cells at different stages of development.  |  Craig, W., et al. 1994. Br J Haematol. 88: 24-30. PMID: 7803253
8. Adhesive interactions in thymic development: does selective expression of CD45 isoforms promote stage-specific microclustering in the assembly of functional adhesive complexes on differentiating T lineage lymphocytes?  |  Pilarski, LM. 1993. Immunol Cell Biol. 71 (Pt 1): 59-69. PMID: 8436412
9. CD45 and RPTPalpha display different protein tyrosine phosphatase activities in T lymphocytes.  |  Ng, DH., et al. 1997. Biochem J. 327 (Pt 3): 867-76. PMID: 9581568
10. Interaction between CD45-AP and protein-tyrosine kinases involved in T cell receptor signaling.  |  Motoya, S., et al. 1999. J Biol Chem. 274: 1407-14. PMID: 9880514

The preferred CD45 antibody is CD45 Antibody (35-Z6): sc-1178.