Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD42b Double Nickase Plasmid (h): sc-401273-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD42b Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD42b Double Nickase Plasmid (h) and CD42b Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GP1BA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD42b Antibody (E-8): sc-271171
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD42b Double Nickase Plasmid (h) | sc-401273-NIC | 20 µg | $410.00 | |||
CD42b Double Nickase Plasmid (h2) | sc-401273-NIC-2 | 20 µg | $410.00 |
GP1BA encodes platelet glycoprotein Ib alpha (CD42b), a key subunit of the GPIb-IX-V receptor complex that mediates platelet tethering to von Willebrand factor under high shear. CD42b-driven adhesion initiates signaling programs that coordinate platelet activation, cytoskeletal remodeling, and integrin engagement, linking hemostatic responses to thromboinflammatory pathways. This axis intersects with mechanotransduction and receptor-proximal signaling networks that regulate platelet aggregation and thrombus stability. Genetic or functional perturbation of GP1BA is associated with inherited platelet adhesion defects and altered bleeding or thrombosis-related phenotypes, making it a relevant target for mechanistic studies of platelet biology.
CD42b Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GP1BA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GP1BA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GP1BA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GP1BA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.