CD30 CRISPR/Cas9 KO Plasmid (m): sc-423446
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD30 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD30 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD30 Antibody (C-3): sc-46683
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD30 CRISPR/Cas9 KO Plasmid (m) | sc-423446 | 20 µg | $397.00 |
Overview
Mouse Tnfrsf8 encodes CD30, a TNF receptor superfamily member expressed on activated lymphocytes that transduces signals through adaptor proteins such as TRAFs to modulate NF-κB and MAPK pathway activity. CD30-dependent signaling influences T cell and B cell activation programs, cytokine production, and survival decisions that shape immune homeostasis. In murine models, altered CD30 function has been used to interrogate mechanisms of lymphocyte differentiation, immune regulation, and inflammation within lymphoid tissues. Dysregulated CD30-associated pathways are relevant to studies of aberrant immune activation and lymphoproliferative phenotypes, supporting its utility as a genetic node for immunology research.
CD30 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tnfrsf8 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tnfrsf8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tnfrsf8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD30 protein expression.
This CRISPR knockout system enables efficient generation of Tnfrsf8-deficient cell models for investigation of CD30 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tnfrsf8 exon(s) critical for CD30 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tnfrsf8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD30 CRISPR/Cas9 KO Plasmid (m) and CD30 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tnfrsf8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD30 HDR Plasmid (m) and CD30 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tnfrsf8 homology arms to support homology-directed repair at defined Tnfrsf8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.