CD3抗体 (5B2) 推荐用于 mouse, rat 和 human 来源的CD3 IP, IF 和 FCM检测

CD3 抗体 (5B2): sc-70626

CD3抗体 (5B2) is rated 5.0 out of 5 by 2.
  • y_2020, m_11, d_24, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_zh_CN, sid_70626, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 133ms
  • REVIEWS, PRODUCT
5
2
4
0
3
0
2
0
1
0

说明书

    • CD3 抗体 5B2 是小鼠单克隆 IgG3,提供 100 µg/ml
    • raised against human peripheral lymphocytes
    • 推荐用于 mouse, rat 和 human 来源的CD3 IP, IF 和 FCM检测
    • 还可偶联FITC 用于IF, IHC(P) 和FCM
    • 看 CD3 (PC3/188A): sc-20047,可查看有标记的 CD3 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790

Should I permeabilize the cells if I want use the 5B2 for FACS analysis?

Asked by: Elenarb000
Thank you for your question. Yes, we recommend fixing and permeabilizing when using this mouse monoclonal antibody, CD3 (5B2): sc-70626, for flow cytometry (FCM) for FACS applications. A link to our recommended protocol for FCM is provided below. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-09-20

I am using CD3 (5B2): sc-70626 monoclonal antibody for immunoprecipitation, how much antibody should I use?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-04-05
  • y_2020, m_11, d_25, h_17CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_70626, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 153ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from IF publication with rat C6 cells IF publication with rat C6 cells. -SCBT Publication Review
Date published: 2015-05-01
Rated 5 out of 5 by from CD3 (5B2) was used successfully in mouse D1 and D1 CD3 (5B2) was used successfully in mouse D1 and D1-CD4 T cells for immunoprecipitation. -SCBT Publication Review
Date published: 2013-04-25
  • y_2020, m_11, d_24, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_zh_CN, sid_70626, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT

圣克鲁斯生物技术在生物医药研究市场的产品开发方面处于世界领先地位。欢迎 致电我们的免费电话1-800-457-3801。 版权 ©, 2007-2020圣克鲁斯生物技术。版权所有。“Santa Cruz Biotechnology”,圣克鲁斯生物技 术的 商标,“Santa Cruz Animal Health”,“San Juan Ranch”,“Supplement of Champions”,San Juan Ranch的商标,“Ultracruz”,“Chemcruz”,“Immunocruz”,“Exactacruz”和“EZ Touch”都是圣克鲁斯生物技术的注册商标。所有的商标都归其各自拥有者所有。