CD2AP CRISPR/Cas9 KO Plasmid (m): sc-419542
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD2AP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD2AP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD2AP CRISPR/Cas9 KO Plasmid (m) | sc-419542 | 20 µg | $397.00 |
Overview
Cd2ap encodes CD2-associated protein (CD2AP), an adaptor and scaffold that coordinates actin cytoskeleton remodeling with membrane trafficking at specialized cell junctions. In mouse cells, CD2AP participates in endocytosis and vesicular sorting through interactions with slit diaphragm and endocytic machinery, supporting cellular polarity and barrier functions. CD2AP-linked networks intersect with signaling pathways that govern adhesion dynamics and cytoskeletal organization, making it a useful node for studying junctional stability and receptor turnover. Genetic perturbation of Cd2ap has been associated with glomerular filtration barrier defects and proteinuric kidney phenotypes, and it is also used to probe immune synapse organization and epithelial integrity in disease-relevant models.
CD2AP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cd2ap gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cd2ap together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cd2ap open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD2AP protein expression.
This CRISPR knockout system enables efficient generation of Cd2ap-deficient cell models for investigation of CD2AP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cd2ap exon(s) critical for CD2AP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cd2ap genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD2AP CRISPR/Cas9 KO Plasmid (m) and CD2AP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cd2ap locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD2AP HDR Plasmid (m) and CD2AP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cd2ap homology arms to support homology-directed repair at defined Cd2ap target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.