Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD21 Double Nickase Plasmid (h): sc-401352-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD21 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD21 Double Nickase Plasmid (h) and CD21 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CR2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD21 Antibody (A-3): sc-13135
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD21 Double Nickase Plasmid (h) | sc-401352-NIC | 20 µg | $410.00 | |||
CD21 Double Nickase Plasmid (h2) | sc-401352-NIC-2 | 20 µg | $410.00 |
Human CR2 encodes CD21, a complement receptor expressed predominantly on B cells and follicular dendritic cells that binds C3d-tagged antigens to amplify B cell receptor signaling. CD21 forms a coreceptor complex with CD19 and CD81 to lower activation thresholds and coordinate antigen capture, trafficking, and presentation within germinal center reactions. Through complement-dependent opsonization and immune complex handling, CD21 contributes to adaptive immune maturation and immunological memory. Altered CR2/CD21 expression or function has been associated with dysregulated humoral immunity and has been studied in contexts such as autoimmune inflammation and B cell–linked malignancy biology.
CD21 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CR2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CR2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CR2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CR2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.