Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD1D Double Nickase Plasmid (h): sc-402435-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD1D Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD1D Double Nickase Plasmid (h) and CD1D Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CD1D. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD1D Antibody (C3D5): sc-19632
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD1D Double Nickase Plasmid (h) | sc-402435-NIC | 20 µg | $410.00 | |||
CD1D Double Nickase Plasmid (h2) | sc-402435-NIC-2 | 20 µg | $410.00 |
CD1D encodes CD1d, a non-classical MHC class I–like antigen-presenting molecule that binds self and microbial lipid antigens and displays them at the cell surface for recognition by invariant natural killer T (iNKT) cells. Through CD1d–TCR interactions, it coordinates innate-like T cell activation, rapid cytokine release, and downstream cross-talk with dendritic cells, NK cells, and conventional T cells, shaping immune surveillance and tolerance. CD1D function is integrated with endosomal trafficking, lipid loading pathways, and antigen presentation processes that influence inflammatory signaling and tissue immune homeostasis. Altered CD1d-mediated lipid antigen presentation has been implicated in immune dysregulation relevant to infection, tumor immunology, and autoimmune or inflammatory conditions, motivating mechanistic studies in diverse cell types.
CD1D Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CD1D locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CD1D. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CD1D function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CD1D-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.