CD1C Double Nickase Plasmid (h): sc-403813-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD1C Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD1C Double Nickase Plasmid (h) and CD1C Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CD1C. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD1C Antibody (B-6): sc-390980
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD1C Double Nickase Plasmid (h) | sc-403813-NIC | 20 µg | $410.00 | |||
CD1C Double Nickase Plasmid (h2) | sc-403813-NIC-2 | 20 µg | $410.00 |
CD1C encodes CD1c, an MHC class I–like antigen-presenting molecule that binds and displays lipid and glycolipid antigens to T cells, shaping adaptive immune recognition. CD1c is prominently expressed on dendritic cell subsets and participates in endosomal trafficking and antigen-loading processes that connect innate sensing to T-cell priming. Through CD1-restricted antigen presentation, CD1c influences immune polarization and cytokine programs involved in host defense and inflammatory regulation. Altered CD1c-expressing dendritic cell abundance or function has been associated with immune dysregulation in autoimmunity, infection, and tumor-associated inflammation, making CD1C a useful node for mechanistic immunology studies.
CD1C Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CD1C locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CD1C. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CD1C function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CD1C-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.