Date published: 2025-9-6

021-6093-6350

SCBT Portrait Logo
Seach Input

CD14 抗体 (5A3B11B5): sc-58951

4.6(13)
寫評論提問

说明书
  • CD14 抗体 5A3B11B5 是小鼠单克隆 IgG2b κ,CD14抗体, 在26篇文献中引用,规格为200 µg/ml
  • 免疫human物种的重组CD14
  • CD14 抗体 (5A3B11B5) 推荐用于WB, IP, IF 和 IHC(P),检测human 来源的 CD14
  • 抗CD14抗体(5A3B11B5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD14 (5A3B11B5): sc-58951免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 CD14 抗体 (5A3B11B5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD14 抗体 (5A3B11B5) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

関連項目

CD14 抗体(5A3B11B5)是一种 IgG2b κ 小鼠单克隆 CD14 抗体(也称为单核细胞分化抗原 CD14 抗体或 CD14 标记抗体),可通过 WB、IP、IF 和 IHC(P) 检测人源 CD14 蛋白。CD14 抗体(5A3B11B5)既可以是非偶联的抗 CD14 抗体形式,也可以是多种偶联形式的抗 CD14 抗体,包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。脂多糖(LPS)可引发活化的巨噬细胞和单核细胞产生的介质和细胞因子的分泌。CD14 是一种糖基磷脂酰肌醇(GPI)锚定蛋白,存在于单核细胞和多形核白细胞的表面。CD14 作为 LPS 的受体,可导致各种蛋白质的分泌。通过 CD14 受体激活 LPS 中的单核细胞的一个重要成分是"衔接分子",即脂多糖结合蛋白(LBP)。CD14 有两种形式,一种是膜结合型(mCD14),另一种是可溶性型(sCD14)。mCD14 可单独对 LPS 作出应答并促进蛋白质的分泌,而不表达 mCD14 的细胞则无法对 LPS 作出应答。缺乏 mCD14 的细胞在 sCD14 的存在下可对 LPS/LBP 作出应答。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD14 抗体 (5A3B11B5)

sc-58951
200 µg/ml
$316.00

CD14 (5A3B11B5): m-IgG Fc BP-HRP 套装

sc-528632
200 µg Ab; 10 µg BP
$354.00

CD14 (5A3B11B5): m-IgGκ BP-HRP 套装

sc-521062
200 µg Ab, 40 µg BP
$354.00

CD14 抗体 (5A3B11B5) AC

sc-58951 AC
500 µg/ml, 25% agarose
$416.00

CD14 抗体 (5A3B11B5) HRP

sc-58951 HRP
200 µg/ml
$316.00

CD14 抗体 (5A3B11B5) FITC

sc-58951 FITC
200 µg/ml
$330.00

CD14 抗体 (5A3B11B5) PE

sc-58951 PE
200 µg/ml
$343.00

CD14 抗体 (5A3B11B5) Alexa Fluor® 488

sc-58951 AF488
200 µg/ml
$357.00

CD14 抗体 (5A3B11B5) Alexa Fluor® 546

sc-58951 AF546
200 µg/ml
$357.00

CD14 抗体 (5A3B11B5) Alexa Fluor® 594

sc-58951 AF594
200 µg/ml
$357.00

CD14 抗体 (5A3B11B5) Alexa Fluor® 647

sc-58951 AF647
200 µg/ml
$357.00

CD14 抗体 (5A3B11B5) Alexa Fluor® 680

sc-58951 AF680
200 µg/ml
$357.00

CD14 抗体 (5A3B11B5) Alexa Fluor® 790

sc-58951 AF790
200 µg/ml
$357.00

Do you know if this antibody will bind to rat CD14? In this paper: PMID: 20849360 DOI: 10.3109/14653249.2010.515578, they seem to suggest that they used a CD14 antibody from Santa Cruz to detect CD14 in rat blood cells.

Asked by: JTponce
Thank you for your question. sc-58951CD14 (5A3B11B5) is recommended for human . There is a 64%homology in rat.
Answered by: BlakeJ
Date published: 2025-02-21

Hello, Does CD14 Antibody (5A3B11B5) Alexa Fluor® 594 have cross reactivity with mouse? Thank you, Ying

Asked by: YingLi
Thank you for your question. This mouse monoclonal antibody has only been validated for human use at this time, and can not be recommended or warrantied for use on mouse samples. If you have any further questions, please contact Technical Service.
Answered by: Technical Support
Date published: 2018-12-11

Can this antibody be used with the Li-Cor system? What secondary should be used?

Asked by: AbPolly
Thank you for your question. Yes, this antibody can be used with the Li-COR system. We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-01-30
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_58951, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 99ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Works nicely on paraffin sectionsI used this to stain paraffin embedded lung tissue for a pan macrophage/monocyte marker. It works very well. The antibody is compatible with 10 mM citrate, pH 6.0 antigen retrieval using a microwave for 30 min. Antibody diluted 1:200 and applied for 60 min, secondary antibody was goat-anti-mouse-Cy2 (Jackson Immunoresearch) diluted 1:400 and applied for 60 mins. I used it for dual staining with another antibody.
Date published: 2023-03-08
Rated 5 out of 5 by from Ottimo funzionamentoHo testato questo prodotto e sono soddisfatta in quanto esce un'unica banda relativa alla proteina
Date published: 2022-04-01
Rated 5 out of 5 by from great antibodyI used this antibody on immunohistochemistry of paraffin embedded tissues and worked great.
Date published: 2017-09-19
Rated 4 out of 5 by from Good antibodyI used this antibody to test the changes in T cell acute lymphoblastic leukemia cells like jurkat,it is a Good antibody for WB
Date published: 2017-06-21
Rated 4 out of 5 by from UNclearit is raised in human origin,so i failed to get a chear band in rat
Date published: 2017-03-16
Rated 5 out of 5 by from cd14,membrane-boundCD14, which we procured in accordance with the orientation test requirements, was glycosylphosphatidylinositol-anchored protein found on the surface of monocytes and polymorphonuclear leukocytes. In fact there are two forms of CD14, membrane-bound and soluble forms, and we use the former
Date published: 2017-02-06
Rated 5 out of 5 by from Great CD14 monoclonal choice for WB and IHC(P)good cytoplasmic immunoperoxidase staining in formalin fixed, paraffin-embedded human lymph node tissue
Date published: 2017-01-14
Rated 5 out of 5 by from ExcellentThere was very strong detection of the ~87 kDa target protein in mouse tissues. Products have a good auxiliary effect, worthy of attention.
Date published: 2017-01-10
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_zh_CN, sid_58951, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
CD14 抗体 (5A3B11B5) is rated 4.6 out of 5 by 13.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_zh_CN, sid_58951, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 104ms
  • REVIEWS, PRODUCT