Date published: 2025-9-7
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caspase-9 Antibody (96.1.23) - Western Blotting - Image 16448
caspase-9 抗体 (96. 1. 23): sc-56076. 在 HeLa (A), Jurkat (B), 石硫合剂处理的 Jurkat (C), 石硫合剂处理的 HeLa (D), 紫外线处理的 HeLa (E) 和 MOLT-4 (F) 全细胞裂解液中对 procaspase-9 表达的 Western 印迹分析.
caspase-9 抗体 (96. 1. 23): sc-56076. 在 HeLa (A), Jurkat (B), 石硫合剂处理的 Jurkat (C), 石硫合剂处理的 HeLa (D), 紫外线处理的 HeLa (E) 和 MOLT-4 (F) 全细胞裂解液中对 procaspase-9 表达的 Western 印迹分析.

caspase-9 抗体 (96.1.23): sc-56076

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寫評論提問

说明书
  • caspase-9 抗体 96.1.23 是小鼠单克隆 IgG1 κ，caspase-9抗体, 在114篇文献中引用，规格为200 µg/ml
  • raised against the prodomain of caspase-9 of human origin
  • caspase-9 抗体 (96.1.23) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 caspase-9
  • 抗caspase-9抗体（96.1.23）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取caspase-9 (96.1.23): sc-56076的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 caspase-9 抗体 (96.1.23) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与caspase-9 抗体 (96.1.23) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Caspase 抗体 包括 caspase-1, caspase-10, caspase-11, caspase-12, caspase-14, caspase-2, caspase-3, caspase-4, caspase-5和caspase-6

Caspase-9抗体（96.1.23）是一种鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人源的caspase-9蛋白。抗半胱氨酸蛋白酶-9抗体（96.1.23）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Caspase-9在细胞凋亡途径中起着至关重要的作用，它作为启动酶激活下游效应酶，如caspase-3和caspase-7，从而促进细胞程序性死亡。这个过程维持细胞内环境稳定，并消除受损或不需要的细胞，这在癌症和神经退行性疾病中尤为重要。Caspase-9的激活受到严格调控，并响应细胞凋亡信号而发生，这凸显了其在细胞应激和损伤反应中的重要性。了解半胱天冬酶-9的功能及其与凋亡级联反应中其他蛋白的相互作用，有助于我们制定针对各种疾病中细胞凋亡的治疗策略。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

caspase-9 抗体 (96.1.23) 参考文献:

  1. caspase-3 和 XIAP 对 Apaf-1/caspase-9 细胞凋亡小体的调控  |  Zou, H., et al. 2003. J Biol Chem. 278: 8091-8. PMID: 12506111
  2. HAX-1抑制缺氧再氧合过程中线粒体后半胱天冬酶-9的激活和细胞死亡。  |  Shaw, J. and Kirshenbaum, LA. 2006. Circ Res. 99: 336-8. PMID: 16917098
  3. 一种基于Caspase 9的Rapamycin激活自杀基因。  |  Stavrou, M., et al. 2018. Mol Ther. 26: 1266-1276. PMID: 29661681
  4. 半胱天冬酶-9是骨细胞迁移的积极调节因子，通过diaPASEF蛋白质组学鉴定。  |  Říhová, K., et al. 2024. J Proteome Res. 23: 2999-3011. PMID: 38498986
  5. DNA 链断裂诱导的聚（ADP-核糖）聚合酶翻译后修饰。  |  Lindahl, T., et al. 1995. Trends Biochem Sci. 20: 405-11. PMID: 8533153
  6. ICE-LAP3是草履虫细胞死亡蛋白Ced-3的哺乳动物同源物，在Fas和肿瘤坏死因子诱导的细胞凋亡过程中被激活。  |  Duan, H., et al. 1996. J Biol Chem. 271: 1621-5. PMID: 8576161
  7. Apopain/CPP32 可裂解细胞修复所必需的蛋白质：细胞凋亡的基本原理。  |  Casciola-Rosen, L., et al. 1996. J Exp Med. 183: 1957-64. PMID: 8642305
  8. ICE-LAP6 是 ICE/Ced-3 基因家族的一个新成员，它被细胞毒性 T 细胞蛋白酶颗粒酶 B 激活。  |  Duan, H., et al. 1996. J Biol Chem. 271: 16720-4. PMID: 8663294
  9. Mch2 alpha 而非 CPP32 对层粘蛋白 A 的裂解：多种白细胞介素 1 β-转化酶相关蛋白酶在细胞凋亡过程中具有不同的底物识别特性。  |  Takahashi, A., et al. 1996. Proc Natl Acad Sci U S A. 93: 8395-400. PMID: 8710882
  10. Mch4体外激活CPP32和Mch3，Mch4是一种新型人类凋亡半胱氨酸蛋白酶，含有两个类似FADD的结构域。  |  Fernandes-Alnemri, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 7464-9. PMID: 8755496
  11. 多聚（ADP-核糖）聚合酶在与分化相关的 DNA 复制过程中的表达表明，它是多蛋白 DNA 复制复合体的一个组成部分。  |  Simbulan-Rosenthal, CM., et al. 1996. Biochemistry. 35: 11622-33. PMID: 8794742
  12. Caspases：细胞凋亡的刽子手。  |  Cohen, GM. 1997. Biochem J. 326 (Pt 1): 1-16. PMID: 9337844

订购信息

产品名称产品编号规格价格数量收藏夹

caspase-9 抗体 (96.1.23)

sc-56076
200 µg/ml
$316.00

caspase-9 (96.1.23): m-IgG Fc BP-HRP 套装

sc-526875
200 µg Ab; 10 µg BP
$354.00

caspase-9 (96.1.23): m-IgG1 BP-HRP 套装

sc-532248
200 µg Ab; 20 µg BP
$354.00

caspase-9 抗体 (96.1.23) AC

sc-56076 AC
500 µg/ml, 25% agarose
$416.00

caspase-9 抗体 (96.1.23) HRP

sc-56076 HRP
200 µg/ml
$316.00

caspase-9 抗体 (96.1.23) FITC

sc-56076 FITC
200 µg/ml
$330.00

caspase-9 抗体 (96.1.23) PE

sc-56076 PE
200 µg/ml
$343.00

caspase-9 抗体 (96.1.23) Alexa Fluor® 488

sc-56076 AF488
200 µg/ml
$357.00

caspase-9 抗体 (96.1.23) Alexa Fluor® 546

sc-56076 AF546
200 µg/ml
$357.00

caspase-9 抗体 (96.1.23) Alexa Fluor® 594

sc-56076 AF594
200 µg/ml
$357.00

caspase-9 抗体 (96.1.23) Alexa Fluor® 647

sc-56076 AF647
200 µg/ml
$357.00

caspase-9 抗体 (96.1.23) Alexa Fluor® 680

sc-56076 AF680
200 µg/ml
$357.00

caspase-9 抗体 (96.1.23) Alexa Fluor® 790

sc-56076 AF790
200 µg/ml
$357.00

I cannot detect Pro-caspase 9 and Caspase 9 bands in HT29 cells. I would like to ask what I should use to dilute the antibody (3% BSA or 3% skim milk), what blocking buffer to use (3% BSA or 3% skim milk), the recommended dilution ratio for this cell type

Asked by: kimanhxinh
Thank you for your question. For assistance please contact Technical service .Please call 800-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-04-04

Is this cas9 antibody(caspase-9 抗体 (96.1.23): sc-56076) suitable for immunofluorescence in frozen section of mouse? The protein what I want to detect is exogenous by caspase-9 CRISPR technique. Is this antibody suitable?

Asked by: Miss Wang
Thank you for your question. Yes, caspase-9 抗体 (96.1.23): sc-56076 is suitable for immunofluorescence in frozen section of mouse. This antibody is raised against the prodomain of caspase-9 of human origin, we did not test it on exogenous cas9 by caspase-9 CRISPR technique.
Answered by: Technical Service SH
Date published: 2022-01-13

What secondary should be used with this antibody?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-12

Can this antibody be used to stain mouse tissue in IHC? Are there any mouse on mouse background issues?

Asked by: AbPolly
Thank you for your question. Yes, this antibody is appropriate for use with mouse tissue. In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to HRP, PE, FITC, AF488 and AF647.
Answered by: Technical Support 9
Date published: 2017-01-12
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Rated 5 out of 5 by from opinions about antibodiesVery good antibodies, work at delal dilutions, no non-specific binding
Date published: 2023-05-24
Rated 5 out of 5 by from Worth recommending!I bought this tube of antibody two years ago, and I have published Bioactive Materials about this research. The title of DNA APtamer S11e, and recognizes fibrosarcoma and acts as a tumor suppressor. Worth recommending!
Date published: 2022-03-13
Rated 5 out of 5 by from Great featuresI tested the antibody on a western blot with Huh-7 cells, it worked very well, at the 1:1000 dilution, giving a clean signal and recognizing both the full length and the cleaved fragment.
Date published: 2021-05-05
Rated 1 out of 5 by from sadly it didn't workWe used it for WB but sadly it didn't give us the results we expected
Date published: 2019-04-01
Rated 2 out of 5 by from Ok. But messy.Multiple band through western blotting, not very clean.
Date published: 2018-07-06
Rated 5 out of 5 by from Caspasa 9Muy buen anticuerpo, funciona muy bien para WB en intestino de rata.
Date published: 2018-06-18
Rated 4 out of 5 by from Worked fineWorked as expected and band intensity was better than contenders..may be 3.5 stars
Date published: 2018-02-01
Rated 5 out of 5 by from clear bandI get this antibody about 2 month ago, I use it on my human retinal endothelial cells lysis. It looks like this antibody can detect both caspase-9 and cleavage caspase-9 at the same time, which is good.
Date published: 2017-11-14
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caspase-9 抗体 (96.1.23) is rated 4.3 out of 5 by 33.
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