casein kinase Iγ3 CRISPR/Cas9 KO Plasmid (h): sc-406678
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- casein kinase Iγ3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the casein kinase Iγ3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
casein kinase Iγ3 CRISPR/Cas9 KO Plasmid (h) | sc-406678 | 20 µg | $397.00 |
Overview
CSNK1G3 encodes casein kinase I gamma 3 (CK1γ3), a membrane-associated serine/threonine protein kinase in the CK1 family that phosphorylates diverse substrates to shape signal transduction, protein turnover, and subcellular trafficking. CK1 isoforms commonly interface with pathways such as Wnt/β-catenin signaling, circadian and cell-cycle control, and receptor-proximal phosphorylation events that influence endocytosis and cytoskeletal organization. Through these roles, CSNK1G3 activity can affect phosphorylation-dependent checkpoints, ubiquitin-mediated degradation, and stimulus-responsive signaling dynamics. Dysregulated kinase signaling and altered phosphorylation networks are frequently linked to proliferative and neurobiological phenotypes, supporting investigation of CSNK1G3 in disease-relevant cellular models without implying clinical outcomes.
casein kinase Iγ3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CSNK1G3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CSNK1G3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CSNK1G3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish casein kinase Iγ3 protein expression.
This CRISPR knockout system enables efficient generation of CSNK1G3-deficient cell models for investigation of casein kinase Iγ3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CSNK1G3 exon(s) critical for casein kinase Iγ3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CSNK1G3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by casein kinase Iγ3 CRISPR/Cas9 KO Plasmid (h) and casein kinase Iγ3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CSNK1G3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by casein kinase Iγ3 HDR Plasmid (h) and casein kinase Iγ3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CSNK1G3 homology arms to support homology-directed repair at defined CSNK1G3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.