Date published: 2025-9-11
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Calnexin Antibody (TO-5) - Western Blotting - Image 376374
Calnexin 抗体 (TO-5): sc-80645. MCF7 (A), AN3 CA (B) 和 Caco-2 (C) 全细胞裂解液中 Calnexin 表达的 Western 印迹分析.
Calnexin 抗体 (TO-5): sc-80645. MCF7 (A), AN3 CA (B) 和 Caco-2 (C) 全细胞裂解液中 Calnexin 表达的 Western 印迹分析.

Calnexin 抗体 (TO-5): sc-80645

4.7(6)
寫評論提問

说明书
  • Calnexin抗体(TO-5)是小鼠单克隆IgG1 κ, 在18篇文献中引用，规格为200 µg/ml
  • 与human的Calnexin的氨基酸529-544对应的合成肽杂交
  • Calnexin 抗体 (TO-5) 推荐用于WB, IP, IF, IHC(P) 和 FCM，检测human 来源的 Calnexin
  • 抗Calnexin抗体（TO-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Calnexin (TO-5): sc-80645的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Calnexin 抗体 (TO-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Calnexin 抗体 (TO-5) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

Calnexin抗体（TO-5）是一种IgG1 κ小鼠单克隆Calnexin抗体（也称为CANX抗体），可通过WB、IP、IF、IHC(P)和FCM检测人类来源的Calnexin。Calnexin抗体（TO-5）有非偶联的抗Calnexin抗体形式，以及多种偶联形式的抗Calnexin抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。钙结合蛋白和钙调蛋白（也称为钙网蛋白）是钙结合蛋白，定位于内质网，钙结合蛋白定位于膜，钙调蛋白定位于内腔。钙结合蛋白是一种I型膜蛋白，与内质网中新合成的糖蛋白相互作用。它可能在协助蛋白质组装和保留内质网中未组装蛋白质亚基方面发挥作用。钙调蛋白具有低亲和力和高亲和力的钙结合位点。钙结合蛋白和钙调蛋白都不含钙调素中发现的钙结合"E-F手"基序。钙结合蛋白和钙调蛋白对内质网中糖蛋白的成熟很重要，似乎与许多相同的蛋白质结合。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Calnexin 抗体 (TO-5)

sc-80645
200 µg/ml
$316.00

Calnexin (TO-5): m-IgG Fc BP-HRP 套装

sc-527052
200 µg Ab; 10 µg BP
$354.00

Calnexin (TO-5): m-IgG1 BP-HRP 套装

sc-532425
200 µg Ab; 20 µg BP
$354.00

Calnexin 抗体 (TO-5) AC

sc-80645 AC
500 µg/ml, 25% agarose
$416.00

Calnexin 抗体 (TO-5) HRP

sc-80645 HRP
200 µg/ml
$316.00

Calnexin 抗体 (TO-5) FITC

sc-80645 FITC
200 µg/ml
$330.00

Calnexin 抗体 (TO-5) PE

sc-80645 PE
200 µg/ml
$343.00

Calnexin 抗体 (TO-5) Alexa Fluor® 488

sc-80645 AF488
200 µg/ml
$357.00

Calnexin 抗体 (TO-5) Alexa Fluor® 546

sc-80645 AF546
200 µg/ml
$357.00

Calnexin 抗体 (TO-5) Alexa Fluor® 594

sc-80645 AF594
200 µg/ml
$357.00

Calnexin 抗体 (TO-5) Alexa Fluor® 647

sc-80645 AF647
200 µg/ml
$357.00

Calnexin 抗体 (TO-5) Alexa Fluor® 680

sc-80645 AF680
200 µg/ml
$357.00

Calnexin 抗体 (TO-5) Alexa Fluor® 790

sc-80645 AF790
200 µg/ml
$357.00

Do you have any data demonstrating the cross-reactivity of the anti-Calnexin clone TO-5 to mouse cells or tissue? The paper you reference for WB on a mouse cell line (pubmed ID 23660595) shows no calnexin bands ... the data all negative.

Asked by: Dr Flo
Thank you for your question. We do not currently have any data using this monoclonal antibody, Calnexin (TO-5): sc-80645, in mouse or rat samples. We will reevaluate the species reactivity for this product and update our product information accordingly. If you have already purchased the antibody, please contact our Technical Service Department and we can support your purchase for any current recommended species per our product warranty. We will remove the product citation you mentioned for this antibody, as in the paper it does indicate that “Immunoreactive calnexin bands were not seen in any of the plasma membranes examined.” As an alternative, we would recommend using our alternate antibody, Calnexin (E-10): sc-46669. We currently have Western blot data in mouse Neuro-2A whole cell lysate. A link to this alternate product is below as a reference. https://www.scbt.com/scbt/product/calnexin-antibody-e-10
Answered by: Technical Support
Date published: 2017-08-15

Can Calnexin (TO-5): sc-80645 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Calnexin (TO-5): sc-80645 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_80645, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 94ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from good WB antibodythis antibody works well as a loading control of nuclear proteins
Date published: 2018-01-23
Rated 5 out of 5 by from WB: strongWB: strong, clean bands observed in HeLa, A-431, K-562 and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-05-12
Rated 5 out of 5 by from Worked well to immunoprecipitate (and verifyWorked well to immunoprecipitate (and verify via WB) calnexin from human fusion MDCK cells. -SCBT Publication Review
Date published: 2015-04-22
Rated 5 out of 5 by from Used for western blot on murine cell lysatesUsed for western blot on murine cell lysates (MC3T3-E1 cells). -SCBT Publication Review
Date published: 2015-03-15
Rated 4 out of 5 by from Bands at expected MW in transfected lysatesBands at expected MW in transfected lysates. -SCBT QC
Date published: 2015-01-23
Rated 5 out of 5 by from Published results of WB in canine mitralPublished results of WB in canine mitral valve extracts. -SCBT Publication Review
Date published: 2014-01-22
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_80645, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT
Calnexin 抗体 (TO-5) is rated 4.7 out of 5 by 6.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_80645, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 100ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。