CA II Antibody (H-10): sc-133110

CA II Antibody (H-10) is a mouse monoclonal IgG2b antibody that detects carbonic anhydrase II (CA II) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CA II plays a crucial role in the reversible hydration of carbon dioxide, facilitating essential physiological processes such as respiration and acid-base balance, which are vital for maintaining homeostasis in the body. CA II is predominantly located in the cytoplasm, where CA II exhibits the highest turnover rate and broadest tissue distribution among all known human carbonic anhydrase isozymes, making CA II a key player in metabolic processes. The human CA2 gene, which encodes CA II, is mapped to chromosome 8q21, and CA II expression is critical for the proper functioning of various tissues, including the kidneys and brain. CA II′s ability to regulate pH and bicarbonate levels underscores CA II′s importance in both normal physiology and pathological conditions, such as cancer, where altered expression of carbonic anhydrases can influence tumor growth and metastasis. With diverse applications and significant biological relevance, anti-CA II antibody (H-10) is an invaluable tool for researchers studying the roles of carbonic anhydrases in health and disease.

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CA II Antibody (H-10) References:

1. Membrane-associated carbonic anhydrase activity in the brain of CA II-deficient mice.  |  Ridderstråle, Y. and Wistrand, PJ. 2000. J Neurocytol. 29: 263-9. PMID: 11276178
2. Carbonic anhydrase II autoantibody and oxidative stress in rheumatoid arthritis.  |  Alver, A., et al. 2011. Clin Biochem. 44: 1385-9. PMID: 21986594
3. Carbonic anhydrase II mediates malignant behavior of pulmonary neuroendocrine tumors.  |  Zhou, Y., et al. 2015. Am J Respir Cell Mol Biol. 52: 183-92. PMID: 25019941
4. Characterization of the Copper(II) Binding Sites in Human Carbonic Anhydrase II.  |  Nettles, WL., et al. 2015. Inorg Chem. 54: 5671-80. PMID: 26010488
5. Mitochondrial proteomic profiling reveals increased carbonic anhydrase II in aging and neurodegeneration.  |  Pollard, A., et al. 2016. Aging (Albany NY). 8: 2425-2436. PMID: 27743511
6. Carbonic anhydrase II microcrystals suitable for XFEL studies.  |  Lomelino, CL., et al. 2018. Acta Crystallogr F Struct Biol Commun. 74: 327-330. PMID: 29870015
7. Carbonic anhydrase II in complex with carboxylic acid-based inhibitors.  |  Lomelino, CL. and McKenna, R. 2019. Acta Crystallogr F Struct Biol Commun. 75: 166-170. PMID: 30839290
8. Polymorphisms in Brucella Carbonic Anhydrase II Mediate CO2 Dependence and Fitness in vivo.  |  García Lobo, JM., et al. 2019. Front Microbiol. 10: 2751. PMID: 31921002
9. Inhibitory Efficacy of Thiosemicarbazones for Carbonic Anhydrase II (Bovine and Human) as a Target of Calcification and Tumorigenicity.  |  Khan, M., et al. 2022. Curr Pharm Des. 28: 3010-3022. PMID: 35909284
10. Bis-pharmacophore of cinnamaldehyde-clubbed thiosemicarbazones as potent carbonic anhydrase-II inhibitors.  |  Rasool, A., et al. 2022. Sci Rep. 12: 16095. PMID: 36167735

The preferred CA II antibody is CA II Antibody (G-2): sc-48351.