Date published: 2025-9-6
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C3 Antibody (B-9) - Western Blotting - Image 10912
C3 抗体 (B-9): sc-28294. 人重组 C3b 的 Western 印迹分析.
C3 抗体 (B-9): sc-28294. 人重组 C3b 的 Western 印迹分析.

C3 抗体 (B-9): sc-28294

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说明书
  • C3 抗体 B-9 是小鼠单克隆 IgG1 κ，C3抗体, 在60篇文献中引用，规格为200 µg/ml
  • 免疫human物种的C3 precursor的氨基酸541-840
  • C3 抗体 (B-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 C3 precursor, C3a anaphylatoxin, C3 α chain, C3 β chain and C3b α′ chain
  • 抗C3抗体（B-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取C3 (B-9): sc-28294的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 C3 抗体 (B-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与C3 抗体 (B-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Complement Pathway Protein 抗体 包括 C3, C3a / α, C3 α, C3aR, C3d, C4, C4 α, C4 β, C5b-9和C5 β

C3抗体（B-9）是一种小鼠单克隆IgG1 kappa轻链抗体，针对人类补体成分3（C3）蛋白的氨基酸541-840。C3也称为CPAMD1或酰化刺激蛋白（ASP），是补体系统的重要组成部分，在先天免疫中起着至关重要的作用。C3主要在肝脏合成，分泌到血液中，存在于细胞外空间，以非活性前体的形式循环。激活后，C3被分解为C3a和C3b片段；C3a作为过敏毒素，可引发炎症反应，而C3b则与病原体表面结合，促进免疫细胞的吞噬作用。C3位于细胞外，这一点至关重要，因为C3能够立即与入侵的病原体相互作用，激活细胞表面的补体级联反应。C3激活失调会导致自身免疫性疾病，并增加感染的可能性。C3 (B-9) 抗体可识别小鼠、大鼠和人类来源的 C3 前体、C3a 过敏毒素、C3 α 链、C3 β 链和 C3b α′ 链，推荐用于蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）。C3 (B-9) 单克隆抗体是研究补体激活途径及其在多种物种免疫反应中的意义的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

C3 抗体 (B-9) 参考文献:

  1. C3a 活性的调节：人 C3a 受体的内化及其受 C5a 的抑制。  |  Settmacher, B., et al. 1999. J Immunol. 162: 7409-16. PMID: 10358194
  2. C3aR和C5aR受体的配体诱导磷酸化是由 'G蛋白偶联受体激酶 '介导的。  |  Langkabel, P., et al. 1999. Eur J Immunol. 29: 3035-46. PMID: 10508278
  3. C3a无乳毒素受体在哮喘的效应阶段发挥作用。  |  Humbles, AA., et al. 2000. Nature. 406: 998-1001. PMID: 10984054
  4. 人类补体成分 C3：cDNA 编码序列和衍生一级结构。  |  de Bruijn, MH. and Fey, GH. 1985. Proc Natl Acad Sci U S A. 82: 708-12. PMID: 2579379
  5. 丝裂原活化蛋白激酶的激活需要来自人类无乳毒素 C5a 受体的两种信号输入。  |  Buhl, AM., et al. 1995. J Biol Chem. 270: 19828-32. PMID: 7649993
  6. 在实验性李斯特菌脑膜脑炎中，TNF-α介导的神经元上无形体毒素 C5a 受体的表达。  |  Stahel, PF., et al. 1997. J Immunol. 159: 861-9. PMID: 9218605

订购信息

产品名称产品编号规格价格数量收藏夹

C3 抗体 (B-9)

sc-28294
200 µg/ml
$316.00

C3 (B-9): m-IgG Fc BP-HRP 套装

sc-528410
200 µg Ab; 10 µg BP
$354.00

C3 (B-9): m-IgGκ BP-HRP 套装

sc-520784
200 µg Ab, 40 µg BP
$354.00

C3 抗体 (B-9) AC

sc-28294 AC
500 µg/ml, 25% agarose
$416.00

C3 抗体 (B-9) HRP

sc-28294 HRP
200 µg/ml
$316.00

C3 抗体 (B-9) FITC

sc-28294 FITC
200 µg/ml
$330.00

C3 抗体 (B-9) PE

sc-28294 PE
200 µg/ml
$343.00

C3 抗体 (B-9) Alexa Fluor® 488

sc-28294 AF488
200 µg/ml
$357.00

C3 抗体 (B-9) Alexa Fluor® 546

sc-28294 AF546
200 µg/ml
$357.00

C3 抗体 (B-9) Alexa Fluor® 594

sc-28294 AF594
200 µg/ml
$357.00

C3 抗体 (B-9) Alexa Fluor® 647

sc-28294 AF647
200 µg/ml
$357.00

C3 抗体 (B-9) Alexa Fluor® 680

sc-28294 AF680
200 µg/ml
$357.00

C3 抗体 (B-9) Alexa Fluor® 790

sc-28294 AF790
200 µg/ml
$357.00

Hello-Which antibody pairs would you recommend to develop a C3 sandwich ELISA

Asked by: Aaron Stoker
Thank you for your question. We have not validated our antibodies for sandwich ELISA.
Answered by: BlakeJ
Date published: 2024-09-20

hello,i see C3 antibody (B-9): sc-28294 is a mouse monoclonal antibody,how can it detect c3a anaphylatoxin and C3b α′ chain at the same time,if i want to use it in IHC,how can i distinguish it is C3b,C3a or c3?

Asked by: sophia
Thank you for your question. C3 Antibody (B-9) is recommended for detection of C3 precursor, C3a anaphylatoxin, C3 α chain, C3 β chain and C3b α′ chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. If you would like to detect the specific protein, please select the other antibody. Please contact our Technical Service Department if you have any other questions. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Sen Li
Date published: 2023-12-25

hello,can C3 Antibody (B-9): sc-28294 be used to dectect C3a anaphylatoxin in mouse.If so,did you have any such successful examples (western images) to shown to me ?

Asked by: sophia0314
Thank you for your question. Yes, C3 Antibody (B-9): sc-28294 is recommended for detection of C3a anaphylatoxin in mouse sample by WB, IP, IF, IHC(P) and ELISA. We don't have an in-house data for detection of C3a anaphylatoxin in mouse sample by WB at this moment, but please refer to the citations for this product.
Answered by: Technical Service SH
Date published: 2023-03-16

Can I use non-transfected HEK-293T as a negative control? In your legend of Near-infrared western blot analysis of C3 expression said 293T has C3 band. But same time in your "sc-28294 HRP. Direct western blot analysis" legend said 293T has no band.

Asked by: Woosung
Thank you for your question. Our Control 293T Lysate: sc-117752 is derived from non-transfected 293T cells and suitable for use as a negative control with our 293T Transfected Lysates. Our Control 293T Lysate goes through the same transfection process as our gene-specific transfected lysates, except without transfecting any cDNA into the cells. The Control 293T Lysate is also used at a lower concentration than regular cell lines for Western blotting. That said, it does appear that HEK293 does not have significant RNA expression of C3 per the Human Protein Atlas. We will review of the Near-infrared Western blot data showing a signal in HEK293T cell lysate, and if it can not be supported we will remove it.
Answered by: Technical Support
Date published: 2021-07-12

Has anyone used this AB for IHC in rat brain tissue? 

Asked by: Anonymous
Thank you for your question. We do not have publications specifically in rat brain tissue, but we have many publication in other rat tissues such as kidney, intesting and heart. We do have publications in mouse brain samples, so I would expect it to work perfectly fine in rat brain as well.
Answered by: Tech Service
Date published: 2021-06-01

Hi - I see that this antibody can detect C3 (which has a molecular weight of 150kDa), but that it is also recommended to detect C3a and C3b. How do I specifically probe for C3a and C3b separately on a western blot? Do I add in something to break C3 down?

Asked by: C3question
Thank you for your question. At this time, we are not able to provide any specific inductions or treatments for samples to increase expression of the different C3 subunits. This is a research-based question, and we would recommend you consult with the primary literature. There are many product citations using this antibody for Western blotting applications, so it may be useful to start by reviewing the citations on our website for this antibody.
Answered by: Technical Support
Date published: 2019-07-12

Has this antibody specificity been validated? If so, can you include the link to evidence/publication? Thank you.

Asked by: Shandiz
Thank you for your question. This antibody has many data images published on this product page, all showing validation. It also has many citations showing it’s use in publications. You can find them here: https://www.scbt.com/scbt/misc/citationResults.jsp ?_requestid=6883845
Answered by: Technical Support
Date published: 2019-05-29

Under what conditions have you demonstrated a positive C3 signal in human paraffin samples? I have tried numerous epitope retrieval protocols and seen nothing. I'm disappointed that you discontinued the goat polyclonal V-20 in favor of this product.

Asked by: squareshot
Thank you for your question. We are sorry to hear that this antibody is not performing for you as expected. We want you to be successful in your research! We use a 1:50 dilution with this antibody on paraffin sections, after a antigen retrieval in sodium citrate with heat. Please contact our Technical Service Department by phone, e-mail or live chat. We are more than happy to help you troubleshoot the antibody. Also, we do warranty our antibodies for one year from the date of purchase, so if we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge, or issue a credit or refund if that is not possible.
Answered by: Tech Service
Date published: 2017-04-12
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Rated 5 out of 5 by from Works well4% PFA fixed, frozen section, rat cochlea, IF microscopy. Worked well.
Date published: 2018-08-01
Rated 1 out of 5 by from Does not detect human C3 in paraffin tissuesI tried using this antibody to detect human C3 in paraffin-embedded tissues, using three different antigen retrieval techniques, and saw nothing. I also tried using the unconjugated antibody followed by an anti-mouse secondary, saw nothing. On the other hand, the discontinued goat polyclonal worked.
Date published: 2017-05-08
Rated 5 out of 5 by from Great for all manner of C3 detection.This is a proven winner in C3 detection. It has broad range or reactivity and great specificity. It will provide you the results and signal you want in all standard applications. It has been published with more than 10 times.
Date published: 2017-01-05
Rated 5 out of 5 by from Superb!!!Great western if and ihc results; strong signal and low background
Date published: 2017-01-05
Rated 4 out of 5 by from IHC-Psome macrophages were positive for C3 by this antobody in IHC-P method
Date published: 2016-12-30
Rated 5 out of 5 by from Great in multiple applicationsThis antibody works very well in Western Blot, IF and IHC(P)
Date published: 2016-12-23
Rated 5 out of 5 by from I used it to detect mouse complement C3bI used it to detect mouse complement C3b with western blot. I should say that it is a wonderful product which gives a clear and concise band without obvious mixed band. I do have bought many of your product (mainly antibodies), they all works well, and the price is acceptable. You helped me a lot in my experiments, and I will keep on using your product.
Date published: 2015-10-15
Rated 5 out of 5 by from Great stain in mouse intestinal tissuesGreat stain in mouse intestinal tissues.
Date published: 2015-08-06
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C3 抗体 (B-9) is rated 4.7 out of 5 by 16.
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