Brn-2/BRN2/POU3F2 Antibody (C-2): sc-393334

Brn-2 Antibody (C-2) is a mouse monoclonal IgG2b antibody that detects Brn-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Brn-2 is a member of the Brn family of transcription factors, specifically classified as a class III POU domain protein, and plays a pivotal role in the early embryonic development of the central nervous system. Brn-2 is predominantly expressed during the development of the forebrain and is coexpressed in most layer II-V cortical neurons, where Brn-2 is essential for regulating the initiation of radial migration of cortical neurons. This process is crucial for proper brain architecture and function, as Brn-2 ensures that neurons migrate to their appropriate locations during development. Additionally, Brn-2 is implicated in the differentiation of smooth muscle cells, highlighting Brn-2′s importance beyond neuronal development. Brn-2′s structure includes two functional transactivating domains, which facilitate Brn-2′s role in gene regulation, and Brn-2′s interactions with other proteins in the Brn family, such as Brn-3a and Brn-3b, further underscore Brn-2′s significance in transcriptional control. anti-Brn-2 antibody (C-2) is an invaluable tool for researchers studying neuronal development and the molecular mechanisms underlying various neurological conditions.

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Brn-2/BRN2/POU3F2 Antibody (C-2) References:

1. PQBP-1, a novel polyglutamine tract-binding protein, inhibits transcription activation by Brn-2 and affects cell survival.  |  Waragai, M., et al. 1999. Hum Mol Genet. 8: 977-87. PMID: 10332029
2. Combinatorial expression of zebrafish Brn-1- and Brn-2-related POU genes in the embryonic brain, pronephric primordium, and pharyngeal arches.  |  Hauptmann, G. and Gerster, T. 2000. Dev Dyn. 218: 345-58. PMID: 10842361
3. Expression of the POU-domain transcription factors SCIP/Oct-6 and Brn-2 is associated with Schwann cell but not oligodendrocyte remyelination of the CNS.  |  Sim, FJ., et al. 2002. Mol Cell Neurosci. 20: 669-82. PMID: 12213447
4. Cooperative dimerization of the POU domain protein Brn-2 on a new motif activates the neuronal promoter of the human aromatic L-amino acid decarboxylase gene.  |  Dugast-Darzacq, C., et al. 2004. Brain Res Mol Brain Res. 120: 151-63. PMID: 14741405
5. Rb induces a proliferative arrest and curtails Brn-2 expression in retinoblastoma cells.  |  Cobrinik, D., et al. 2006. Mol Cancer. 5: 72. PMID: 17163992
6. Histologic and epidemiologic correlates of P-MAPK, Brn-2, pRb, p53, and p16 immunostaining in cutaneous melanomas.  |  Richmond-Sinclair, NM., et al. 2008. Melanoma Res. 18: 336-45. PMID: 18781132
7. The POU domain transcription factor Brn-2: elevated expression in malignant melanoma and regulation of melanocyte-specific gene expression.  |  Eisen, T., et al. 1995. Oncogene. 11: 2157-64. PMID: 7478537
8. The POU domain transcription factor Brn-2 is required for the determination of specific neuronal lineages in the hypothalamus of the mouse.  |  Nakai, S., et al. 1995. Genes Dev. 9: 3109-21. PMID: 8543155
9. Development and survival of the endocrine hypothalamus and posterior pituitary gland requires the neuronal POU domain factor Brn-2.  |  Schonemann, MD., et al. 1995. Genes Dev. 9: 3122-35. PMID: 8543156
10. Redox regulation of Brn-2/N-Oct-3 POU domain DNA binding activity and proteolytic formation of N-Oct-5 during melanoma cell nuclear extraction.  |  Smith, AG., et al. 1998. Melanoma Res. 8: 2-10. PMID: 9508370

The preferred Brn-2/BRN2/POU3F2 antibody is Brn-2/BRN2/POU3F2 Antibody (B-2): sc-393324.