Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BHMT Double Nickase Plasmid (h): sc-405772-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BHMT Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- BHMT Double Nickase Plasmid (h) and BHMT Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BHMT. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BHMT Antibody (H-7): sc-390299
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BHMT Double Nickase Plasmid (h) | sc-405772-NIC | 20 µg | $410.00 | |||
BHMT Double Nickase Plasmid (h2) | sc-405772-NIC-2 | 20 µg | $410.00 |
Human BHMT (betaine–homocysteine S-methyltransferase) is a cytosolic methyltransferase that catalyzes remethylation of homocysteine to methionine using betaine as a methyl donor, linking one‑carbon metabolism to methionine/S-adenosylmethionine homeostasis. Through regulation of homocysteine flux, BHMT influences cellular methylation capacity, redox balance, and osmolyte metabolism, with downstream effects on epigenetic regulation and hepatic metabolic programs. Altered BHMT activity has been associated with hyperhomocysteinemia and disruptions in methyl-group balance observed in metabolic and liver-related disorders, making it a useful node for studying methylation-dependent pathways and nutrient–gene interactions.
BHMT Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BHMT locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BHMT. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BHMT function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BHMT-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.