Date published: 2025-9-10

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β-glucuronidase 抗体 (E-11): sc-374629

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说明书
  • beta-glucuronidase 抗体 E-11 是小鼠单克隆 IgG1 κ,β-glucuronidase抗体, 在2篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列352-651位于human物种的β-glucuronidase的C-terminus
  • beta-glucuronidase 抗体 (E-11) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 β-glucuronidase
  • 抗beta-glucuronidase抗体(E-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取β-glucuronidase (E-11): sc-374629免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 β-glucuronidase 抗体 (E-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与β-glucuronidase 抗体 (E-11) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品,请参阅我们的小鼠 IgG 结合蛋白完整列表。

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関連項目

β-葡萄糖醛酸酶抗体(E-11)是一种IgG1 κ小鼠单克隆β-葡萄糖醛酸酶抗体(也被称为β-葡萄糖醛酸酶抗体),可通过WB、IP、IF、IHC(P)和ELISA检测人源的β-葡萄糖醛酸酶蛋白。β-葡萄糖醛酸酶抗体(E-11)既可以非偶联抗β-葡萄糖醛酸酶抗体的形式提供,也可以多种偶联形式的抗β-葡萄糖醛酸酶抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。β-葡萄糖醛酸酶催化β-D-葡萄糖醛酸苷和水转化为醇和D-葡萄糖醛酸。β-葡萄糖醛酸酶缺乏是人类溶酶体贮积症粘多糖病VII型(MPS VII)的病因。具体来说,两个残基对催化活性很重要:Glu 451和Glu 540。这些位点的突变会影响蛋白质的整体结构,该蛋白质通常由每个启动子包括一个果冻卷筒、一个免疫球蛋白恒定域和一个TIM卷筒的同源四聚体组成。β-葡萄糖醛酸酶活性的调节可能在肿瘤发生和多种癌症的侵袭性中发挥作用,也是开发需要内源性酶裂解活性细胞抑制剂以发挥抗肿瘤活性的功能性前药的重要因素。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

β-glucuronidase 抗体 (E-11) 参考文献:

  1. 人口腔鳞状细胞癌异种移植物中的肝素酶和肿瘤侵袭模式  |  Kurokawa, H., et al. 2003. Cancer Sci. 94: 277-85. PMID: 12824922
  2. 维拉帕米能调节 HepG2 细胞中人 beta-葡萄糖醛酸酶的活性和 mRNA 表达。  |  Grube, M., et al. 2003. Naunyn Schmiedebergs Arch Pharmacol. 368: 463-9. PMID: 14618298
  3. 大鼠和小鼠 beta-葡糖醛酸酶 mRNA 和 cDNA 的特性,包括序列多态性和 mRNA 水平遗传调节的证据。  |  Watson, G., et al. 1985. Gene. 36: 15-25. PMID: 3840761
  4. 在大肠杆菌中分离和表达编码人类 beta-葡糖醛酸酶的 cDNA 克隆。  |  Guise, KS., et al. 1985. Gene. 34: 105-10. PMID: 3924735
  5. 癫痫发作活动, 糖尿病和其他一些疾病患者血清中的β-葡萄糖醛酸酶。  |  Varma, R., et al. 1983. Neurosci Lett. 39: 105-11. PMID: 6355907
  6. 人类精浆中β-葡萄糖醛酸酶主要形式的分离和特征描述。  |  Gupta, GS. and Singh, GP. 1983. Biochim Biophys Acta. 748: 398-404. PMID: 6416296
  7. 利用免疫亲和层析技术纯化大鼠肝脏微粒体和溶酶体中的β-葡萄糖醛酸酶并确定其特征。  |  Himeno Mnishimura, Y., et al. 1976. Eur J Biochem. 70: 349-59. PMID: 64358
  8. 人类 beta-葡萄糖醛酸酶的结构揭示了候选溶酶体靶向和活性位点基团。  |  Jain, S., et al. 1996. Nat Struct Biol. 3: 375-81. PMID: 8599764
  9. 轻度粘多糖病 VII 型, 假缺失症和一个杂合子中的β-葡萄糖醛酸酶酶活性低和人类β-葡萄糖醛酸酶基因突变。  |  Vervoort, R., et al. 1998. Hum Genet. 102: 69-78. PMID: 9490302

订购信息

产品名称产品编号规格价格数量收藏夹

β-glucuronidase 抗体 (E-11)

sc-374629
200 µg/ml
$316.00

β-glucuronidase (E-11): m-IgGκ BP-HRP 套装

sc-522796
200 µg Ab, 40 µg BP
$354.00

β-glucuronidase 抗体 (E-11) AC

sc-374629 AC
500 µg/ml, 25% agarose
$416.00

β-glucuronidase 抗体 (E-11) HRP

sc-374629 HRP
200 µg/ml
$316.00

β-glucuronidase 抗体 (E-11) FITC

sc-374629 FITC
200 µg/ml
$330.00

β-glucuronidase 抗体 (E-11) PE

sc-374629 PE
200 µg/ml
$343.00

β-glucuronidase 抗体 (E-11) Alexa Fluor® 488

sc-374629 AF488
200 µg/ml
$357.00

β-glucuronidase 抗体 (E-11) Alexa Fluor® 546

sc-374629 AF546
200 µg/ml
$357.00

β-glucuronidase 抗体 (E-11) Alexa Fluor® 594

sc-374629 AF594
200 µg/ml
$357.00

β-glucuronidase 抗体 (E-11) Alexa Fluor® 647

sc-374629 AF647
200 µg/ml
$357.00

β-glucuronidase 抗体 (E-11) Alexa Fluor® 680

sc-374629 AF680
200 µg/ml
$357.00

β-glucuronidase 抗体 (E-11) Alexa Fluor® 790

sc-374629 AF790
200 µg/ml
$357.00

Hi there. Is this antibody compatible with BSA blocking or dry milk blocking reagents? I know there is a recommended specific blocking solution but am wondering if it is essential. Thank you.

Asked by: avutz
Thank you for your question. While results will be best with our UltraCruz® Blocking Reagent: sc-516214, other options for blocking can also be employed instead. Your best course of action would be a preliminary experiment in which you compare results from BSA- v Blotto-based blocking on your particular samples.
Answered by: Tech Support Europe
Date published: 2022-10-06

请问这个能识别鼠源的β-glucuronidase?我们希望找一个特异性识别人源的内参蛋白

Asked by: qw04
感谢您的提问。 sc-374629没有检测和小鼠的结合效率。不能保证它只和人类结合。如果您可以直接给我们asia@scbio.cn发电子邮件,那将会很有帮助。这样我们可以对这个问题和其他相关问题进行更多的讨论。 Thank you for your question. Sc-374629 was not tested for binding efficiency with mice. There is no guarantee that it will only bind to humans.It would be helpful if you could email us directly at asia@scbio.cn. So we can have a more interactive discussion about this and other related issues.
Answered by: Zhanglong
Date published: 2022-03-07

鼠源的肝组织可以反应吗?

Asked by: irisrjy
感谢您的提问。β-glucuronidase Antibody (E-11): sc-374629 不推荐用于鼠的样本。Thank you for your question. β-glucuronidase Antibody (E-11): sc-374629 is not recommended for detection of mouse sample.
Answered by: Technical Support 8
Date published: 2018-12-21

I am using β-glucuronidase (E-11): sc-374629 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Specific detection in WBFor IF in cold MeOH fixed cells and 4% PFA-fixed cells, I could not see obvious staining, but I detected specific a band by WB in over exposure. I think this antibody worked very well.
Date published: 2017-02-02
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human liver tissue. -SCBT QC
Date published: 2015-02-22
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β-glucuronidase 抗体 (E-11) is rated 5.0 out of 5 by 3.
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