Bcl-2 (h): 293T Lysate: sc-176463

Bcl-2 (h): 293T Lysate is derived from the 293T cell line, a human embryonic kidney cell line engineered to express the SV40 large T antigen, enhancing its transfection efficiency and protein expression. This lysate is specifically enriched with human Bcl-2, a key anti-apoptotic protein that regulates cell death by inhibiting the mitochondrial apoptotic pathway. Bcl-2 interacts with pro-apoptotic proteins like Bax and Bak, preventing the release of cytochrome c from the mitochondria and thus blocking the caspase cascade that leads to apoptosis. In research, Bcl-2 (h): 293T Lysate is utilized to study the molecular mechanisms of apoptosis regulation and the role of Bcl-2 in cell survival. Researchers use this lysate in Western blotting to detect and quantify Bcl-2 expression under different experimental conditions, providing insights into its regulation and post-translational modifications. The lysate is also employed in co-immunoprecipitation assays to identify proteins that interact with Bcl-2, helping to explain its role in the apoptotic signaling network and its interactions with other members of the Bcl-2 family. Additionally, functional assays using Bcl-2 (h): 293T Lysate are conducted to assess the impact of Bcl-2 on apoptosis in response to various stimuli. These studies contribute to understanding how Bcl-2 modulates cell death and survival pathways, offering insights into fundamental processes of cell biology. The use of Bcl-2 (h): 293T Lysate allows for detailed investigations into apoptosis regulation, providing valuable data for understanding the complex balance between cell survival and programmed cell death. The origin and quality of the 293T cell line are rigorously controlled to ensure consistent and reproducible results in experimental research.