Date published: 2025-9-6

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BAF53 抗体 (E-3): sc-137062

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说明书
  • BAF53 抗体 E-3 是小鼠单克隆 IgG1 κ,BAF53抗体, 在13篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的BAF53的N-terminus附近的氨基酸87-129之间
  • BAF53 抗体 (E-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 BAF53; 也和以下物种反应,包括: 和 canine, bovine and porcine
  • 可用于ChIP的TransCruz试剂(sc-137062 X, 200 µg/0.1 ml)
  • 抗BAF53抗体(E-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取BAF53 (E-3): sc-137062免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 BAF53 抗体 (E-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与BAF53 抗体 (E-3) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

BAF53抗体(E-3)是一种IgG1 κ小鼠单克隆BAF53抗体(也被称为BAF53抗体),它能够通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学(IHC,石蜡切片)和酶联免疫吸附试验(ELISA)检测小鼠、大鼠和人的BAF53蛋白。BAF53抗体(E-3)既提供非偶联的抗BAF53抗体形式,也提供多种偶联形式的抗BAF53抗体,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®偶联物。SWI/SNF复合物通过ATP依赖的染色质重塑来调节基因表达。Brm(SNF2-α)、Brg-1(SNF2-β)、Ini1(整合酶相互作用蛋白1,SNF5)、BAF53(ARPnβ)、BAF57、BAF155(SRG3)和BAF170构成了功能核心。从酵母到人类的BAF53同源物含有一个保守的N端基序,其中包含位于丝氨酸2和酪氨酸6的残基,这些残基在BAF53活性中起重要作用。BAF53蛋白在细胞核和细胞质之间穿梭。BAF53还与TIP49和TIP48形成复合物,介导c-Myc的致癌活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

BAF53 抗体 (E-3) 参考文献:

  1. 由 SWI/SNF 亚基重构核心染色质重塑复合物。  |  Phelan, ML., et al. 1999. Mol Cell. 3: 247-53. PMID: 10078207
  2. 神经母细胞瘤中SMARCF1(SWI1的截短形式)的表达。  |  Takeuchi, T., et al. 2001. Am J Pathol. 158: 663-72. PMID: 11159203
  3. BAF53 可形成不同的核复合体,并在致癌转化过程中作为与 c-Myc 相互作用的关键核辅助因子发挥作用。  |  Park, J., et al. 2002. Mol Cell Biol. 22: 1307-16. PMID: 11839798
  4. 人类核肌动蛋白相关蛋白 hArpNbeta/Baf53 基因的替代剪接产物,在细胞质中编码一种蛋白异构体 hArpNbetaS。  |  Ohfuchi, E., et al. 2002. Biosci Biotechnol Biochem. 66: 1740-3. PMID: 12353638
  5. 染色质修饰复合物的成分 BAF53 的细胞质定域和核-细胞质穿梭。  |  Lee, JH., et al. 2003. Mol Cells. 16: 78-83. PMID: 14503849
  6. 裂殖酵母中的BAF53/Arp4同源蛋白Alp5是组蛋白H4乙酰化、动点-纺锤体附着和中心粒基因沉默所必需的。  |  Minoda, A., et al. 2005. Mol Biol Cell. 16: 316-27. PMID: 15483052
  7. BAF53 的 Ser2 和 Tyr6 突变体对细胞生长和 p53 依赖性转录的影响。  |  Lee, JH., et al. 2005. Mol Cells. 19: 289-93. PMID: 15879716
  8. 在BAF53耗竭的间期细胞中,染色质解聚导致染色体区域的扩张。  |  Lee, K., et al. 2007. Mol Biol Cell. 18: 4013-23. PMID: 17652455
  9. Baf复合物对HIV-1 LTR活性的不同调节。  |  Van Duyne, R., et al. 2011. J Mol Biol. 411: 581-96. PMID: 21699904
  10. 肌动蛋白相关蛋白BAF53对染色体亚域的完整性至关重要。  |  Lee, K., et al. 2015. Mol Cells. 38: 789-95. PMID: 26242195
  11. BAF53a 是一种潜在的预后生物标志物,可促进胶质瘤细胞的侵袭和上皮-间质转化。  |  Meng, L., et al. 2017. Oncol Rep. 38: 3327-3334. PMID: 29039584
  12. 在没有持续 ATP 水解的情况下,人类 SWI/SNF 可维持核小体破坏。  |  Imbalzano, AN., et al. 1996. J Biol Chem. 271: 20726-33. PMID: 8702824

订购信息

产品名称产品编号规格价格数量收藏夹

BAF53 抗体 (E-3)

sc-137062
200 µg/ml
$316.00

BAF53 (E-3): m-IgG Fc BP-HRP 套装

sc-528851
200 µg Ab; 10 µg BP
$354.00

BAF53 (E-3): m-IgGκ BP-HRP 套装

sc-521410
200 µg Ab, 40 µg BP
$354.00

BAF53 (E-3): m-IgG1 BP-HRP 套装

sc-543168
200 µg Ab; 20 µg BP
$354.00

BAF53 抗体 (E-3) X

sc-137062 X
200 µg/0.1 ml
$316.00

BAF53 抗体 (E-3) AC

sc-137062 AC
500 µg/ml, 25% agarose
$416.00

BAF53 抗体 (E-3) HRP

sc-137062 HRP
200 µg/ml
$316.00

BAF53 抗体 (E-3) FITC

sc-137062 FITC
200 µg/ml
$330.00

BAF53 抗体 (E-3) PE

sc-137062 PE
200 µg/ml
$343.00

BAF53 抗体 (E-3) Alexa Fluor® 488

sc-137062 AF488
200 µg/ml
$357.00

BAF53 抗体 (E-3) Alexa Fluor® 546

sc-137062 AF546
200 µg/ml
$357.00

BAF53 抗体 (E-3) Alexa Fluor® 594

sc-137062 AF594
200 µg/ml
$357.00

BAF53 抗体 (E-3) Alexa Fluor® 647

sc-137062 AF647
200 µg/ml
$357.00

BAF53 抗体 (E-3) Alexa Fluor® 680

sc-137062 AF680
200 µg/ml
$357.00

BAF53 抗体 (E-3) Alexa Fluor® 790

sc-137062 AF790
200 µg/ml
$357.00

BAF53 (E-3) 中和勝肽

sc-137062 P
100 µg/0.5 ml
$68.00

Hi, I observed double bands in blots using mouse liver and human 293T cell lysate. One is ~45 kDa and the other is ~40 kDa. Why is this happening? Which size band should I trust?

Asked by: Chowon
Thank you for your question. To troubleshoot the antibody please contact our technical service department by emailing scbt@scbt.com or call (800)-457-3801.
Answered by: BlakeJ
Date published: 2024-07-29

What application is the blocking peptide sc-137062 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Clear band in WBVery clear and strong band in WB of KM12 and HeLa cells (50 ug protein, 1:500, in 5% Milk/TBST)
Date published: 2018-11-02
Rated 5 out of 5 by from good productIt is a good antibody. We got clean band for WB experiment.
Date published: 2018-08-22
Rated 5 out of 5 by from Muy buenos resultados.Este anticuerpo se usó para detectar la expresión de BAF53 mediante Western Blot. Obtuvimos muy buenos resultados, pudimos observar una banda muy clara y precisa.
Date published: 2017-10-16
Rated 4 out of 5 by from Worked well for western blottingDetects endogenous protein from PC3 nuclear extracts
Date published: 2016-12-07
Rated 5 out of 5 by from Favorable WB signal produced using HeLaFavorable WB signal produced using HeLa, K-562 and Ramos whole cell lysates. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from Produced excellent immunoperoxidase nuclearProduced excellent immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
Date published: 2015-03-09
Rated 4 out of 5 by from Very large robust size bands obtained inVery large robust size bands obtained in WB using mouse BAF53 transfected 293T and Jurkat whole cell lysates -SCBT QC
Date published: 2015-01-04
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BAF53 抗体 (E-3) is rated 4.6 out of 5 by 7.
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