Date published: 2025-9-6

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BAF53 抗体 (C-7): sc-271011

4.7(3)
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说明书
  • BAF53抗体(C-7)是小鼠单克隆IgG1 κ, 在1篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列47-171位于human物种的BAF53的N-terminus附近
  • 推荐用于 mouse, rat 和 human 来源的BAF53 WB, IP, IF 和 ELISA检测
  • 可用于ChIP的TransCruz试剂(sc-271011 X, 200 µg/0.1 ml)
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是BAF53 Antibody (C-7) for WB applications. 的首选辅助检测试剂,这些试剂现与BAF53 Antibody (C-7) 打包提供(请参阅下面的订购信息)。

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関連項目

SWI/SNF复合物通过ATP依赖的染色质重塑来调节基因表达。Brm(SNF2-α)、Brg-1(SNF2-β)、Ini1(整合酶相互作用蛋白1,SNF5)、BAF53(ARPnβ)、BAF57、BAF155(SRG3)和BAF170组成了功能核心。从酵母到人类的BAF53同源物都含有一个保守的N端基序,该基序包含位于第2位丝氨酸和第6位酪氨酸的残基,这些残基在BAF53的活性中发挥着重要作用。BAF53蛋白在细胞核和细胞质之间穿梭。BAF53还与TIP49和TIP48形成复合物,介导c-Myc的致癌活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

BAF53 抗体 (C-7) 参考文献:

  1. 通过荧光原位杂交将SMARCF1基因分配到塔玛袋鼠染色体5q上。  |  Waters, PD., et al. 2001. Cytogenet Cell Genet. 93: 315-6. PMID: 11528131
  2. TIP49通过影响染色质重塑来调节β-catenin介导的肿瘤转化和T细胞因子靶基因的诱导。  |  Feng, Y., et al. 2003. Cancer Res. 63: 8726-34. PMID: 14695187
  3. 由染色质重塑复合体控制的新型 RING 分子 Skeletrophin 在恶性黑色素瘤中下调。  |  Takeuchi, T., et al. 2005. DNA Cell Biol. 24: 339-44. PMID: 15869411
  4. BAF53 的 Ser2 和 Tyr6 突变体对细胞生长和 p53 依赖性转录的影响。  |  Lee, JH., et al. 2005. Mol Cells. 19: 289-93. PMID: 15879716
  5. BAF53与p53相互作用,并参与p53介导的p21基因转录。  |  Wang, M., et al. 2007. J Biochem. 142: 613-20. PMID: 17878219
  6. BAF53与有丝分裂染色体的关联。  |  Lee, K., et al. 2007. Mol Cells. 24: 288-93. PMID: 17978584
  7. 通过核受体共激活因子飞行-I将SWI/SNF染色质重塑复合物招募到类固醇激素调节的启动子中。  |  Jeong, KW., et al. 2009. J Biol Chem. 284: 29298-309. PMID: 19720835
  8. 在宫颈癌细胞中,通过减少BAF53来抑制HPV E6和E7的表达。  |  Lee, K., et al. 2011. Biochem Biophys Res Commun. 412: 328-33. PMID: 21821000
  9. BAF53a 是一种潜在的预后生物标志物,可促进胶质瘤细胞的侵袭和上皮-间质转化。  |  Meng, L., et al. 2017. Oncol Rep. 38: 3327-3334. PMID: 29039584
  10. DNA 损伤通过 CDK9 和 BAF53 触发 RASSF6 抑癌基因的核积累,从而调控 p53 靶基因的转录。  |  Kuleape, JA., et al. 2022. Mol Cell Biol. 42: e0031021. PMID: 34898277

订购信息

产品名称产品编号规格价格数量收藏夹

BAF53 抗体 (C-7)

sc-271011
200 µg/ml
$316.00

BAF53 (C-7): m-IgG Fc BP-HRP 套装

sc-540186
200 µg Ab; 10 µg BP
$354.00

BAF53 (C-7): m-IgG1 BP-HRP 套装

sc-541958
200 µg Ab; 20 µg BP
$354.00

BAF53 抗体 (C-7) X

sc-271011 X
200 µg/0.1 ml
$316.00

What is the recommended starting dilution for Western blot for BAF53 (C-7): sc-271011 monoclonal antibody?

Asked by: cjMara
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-271011.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Faint bands appeared on Western BlotGot a sample for BAF53 protein and did not get great signal. Had to increase intensity drastically to see faint bands. However, bands were crisp and clear.
Date published: 2017-09-18
Rated 5 out of 5 by from Satisfactory Western Blot data of BAF53 expressionSatisfactory Western Blot data of BAF53 expression in HeLa, K-562 and Ramos whole cell lysates. -SCBT QC
Date published: 2015-03-21
Rated 5 out of 5 by from Good Western Blot data of BAF53 expression in nonGood Western Blot data of BAF53 expression in non-transfected and mouse BAF53 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-06-06
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BAF53 抗体 (C-7) is rated 4.7 out of 5 by 3.
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