Date published: 2025-9-7
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ATGL Antibody (F-7) - Western Blotting - Image 354973
ATGL 抗体 (F-7): sc-365278. SJRH30 (A), HeLa (B), K-562 (C), 3T3-L1 (D) 和 A-673 (E) 全细胞裂解液中 ATGL 表达的 Western 印迹分析.
ATGL 抗体 (F-7): sc-365278. SJRH30 (A), HeLa (B), K-562 (C), 3T3-L1 (D) 和 A-673 (E) 全细胞裂解液中 ATGL 表达的 Western 印迹分析.

ATGL 抗体 (F-7): sc-365278

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说明书
  • ATGL 抗体 F-7 是小鼠单克隆 IgG1 κ，ATGL抗体, 在44篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列361-504位于human物种的ATGL的C-terminus
  • ATGL 抗体 (F-7) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 ATGL
  • 抗ATGL抗体（F-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ATGL (F-7): sc-365278的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是ATGL Antibody (F-7) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与ATGL Antibody (F-7) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

ATGL抗体（F-7）是一种IgG1κ小鼠单克隆ATGL抗体（也称为PNPLA2抗体），可以通过WB、IP、IF和ELISA检测来自小鼠、大鼠和人类的ATGL蛋白。ATGL抗体（F-7）以非偶联型抗ATGL抗体形式供应，同时也提供多种偶联型抗ATGL抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Adiponutrin家族成员与肥胖和糖尿病有关，包括Adiponutrin（ADPN）、GS1、GS2、GS2-like、PNPLA1和脂肪甘油三酯脂肪酶（ATGL），后者也被称为Desnutrin或含有Patatin样磷脂酶域蛋白2（PLNPA2）。ATGL是一种含有486个氨基酸的蛋白，在小鼠和人类脂肪组织中高度表达。它含有一个高度保守的180个氨基酸的N端Patatin域，该域与植物酰基水解酶共有的甘氨酸丰富区、天冬氨酸活性位点基序和活性丝氨酸水解酶基序相同。与激素敏感脂肪酶一起，ATGL催化哺乳动物脂肪组织中储存的甘油三酯。ATGL的脂肪酶活性依赖于活化的丝氨酸残基的存在。ADPN和ATGL受胰岛素的相反调节，即禁食时ATGL上调而ADPN下调。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ATGL 抗体 (F-7) 参考文献:

  1. 脂肪组织中的脂肪动员是由脂肪甘油三酯脂肪酶促进的。  |  Zimmermann, R., et al. 2004. Science. 306: 1383-6. PMID: 15550674
  2. 脂肪素家族成员的表达, 调节和甘油三酯水解酶活性。  |  Lake, AC., et al. 2005. J Lipid Res. 46: 2477-87. PMID: 16150821
  3. ATGL介导的脂肪分解代谢通过PPAR-α和PGC-1调节心脏线粒体功能。  |  Haemmerle, G., et al. 2011. Nat Med. 17: 1076-85. PMID: 21857651
  4. ATGL通过SIRT1促进自噬/脂质降解，从而控制肝脂滴分解代谢。  |  Sathyanarayan, A., et al. 2017. Cell Rep. 19: 1-9. PMID: 28380348
  5. ATGL 依赖性白脂肪组织脂肪分解控制肝细胞 PPARα 活性。  |  Fougerat, A., et al. 2022. Cell Rep. 39: 110910. PMID: 35675775
  6. ATGL是一种生物合成酶，用于羟基脂肪酸的脂肪酸酯。  |  Patel, R., et al. 2022. Nature. 606: 968-975. PMID: 35676490
  7. 阻断脂质摄取途径不能预防脂肪甘油三酯脂酶（ATGL）缺乏症中的毒性。  |  Oluwadare, J., et al. 2022. J Lipid Res. 63: 100274. PMID: 36115595

订购信息

产品名称产品编号规格价格数量收藏夹

ATGL 抗体 (F-7)

sc-365278
200 µg/ml
$316.00

ATGL (F-7): m-IgGκ BP-HRP 套装

sc-522189
200 µg Ab, 40 µg BP
$354.00

ATGL 抗体 (F-7) AC

sc-365278 AC
500 µg/ml, 25% agarose
$416.00

ATGL 抗体 (F-7) HRP

sc-365278 HRP
200 µg/ml
$316.00

ATGL 抗体 (F-7) FITC

sc-365278 FITC
200 µg/ml
$330.00

ATGL 抗体 (F-7) PE

sc-365278 PE
200 µg/ml
$343.00

ATGL 抗体 (F-7) Alexa Fluor® 488

sc-365278 AF488
200 µg/ml
$357.00

ATGL 抗体 (F-7) Alexa Fluor® 546

sc-365278 AF546
200 µg/ml
$357.00

ATGL 抗体 (F-7) Alexa Fluor® 594

sc-365278 AF594
200 µg/ml
$357.00

ATGL 抗体 (F-7) Alexa Fluor® 647

sc-365278 AF647
200 µg/ml
$357.00

ATGL 抗体 (F-7) Alexa Fluor® 680

sc-365278 AF680
200 µg/ml
$357.00

ATGL 抗体 (F-7) Alexa Fluor® 790

sc-365278 AF790
200 µg/ml
$357.00

I need to use an antibody to detect the protein in samples of equine origin. Do you know if sc-365278: ATGL (F-7) mouse monoclonal antibody will work?

Asked by: Trav11
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from Ottimo funzionamento!Ho utilizzato il campione di anticorpo per delle prove su cellule di fegato e la risposta è stata molto buona, con un segnale molto pulito.
Date published: 2022-02-02
Rated 5 out of 5 by from Good productThe antibody I bought a couple of days ago was very sensitive.
Date published: 2021-07-20
Rated 3 out of 5 by from Anticorpo discretoProteine caricate su membrana di nitrocellulosa. L'anticorpo è stato usato con diluzione 1:1000 in latte al 5% per una notte a 4°C. Presenza di background aspecifico.
Date published: 2021-04-23
Rated 1 out of 5 by from Does not work very well at all for endogenous ATGLWorked very poorly for ATGL with murine WAT lysate. The band if barely visible using a 1:1000 dilution and 40 ug of protein. Did not at all detect ATGL in 3T3-L1 adipocytes. As state in another review, it gave two bands and it is not easy to know which is correct as they are of similar (very weak) intensity. Essentially useless for my purpose. I wonder if everyone who indicated it worked for their IF are even getting the correct signals. Very disappointing and a waste of about $300.00. I would give less than one star if I could.
Date published: 2020-02-21
Rated 5 out of 5 by from Lab ManagerWe got very good IF results by testing this ATGL (F-7) antibody on mouse brain tissue.
Date published: 2019-11-20
Rated 4 out of 5 by from Works for HeLa cellsWe received a free sample of these antibody from Santa Cruz for evaluation. The antibody was tested in IF and western blot assays with HeLa cells. In IF it shows specific as well as some background non-specific staining, In WB it recognizes a major product of expected Mw, ~55KDa, and some minor bands. Overall, we consider specificity acceptable for our assays and can recommend using it for IF and WB with human cell lines.
Date published: 2018-04-01
Rated 4 out of 5 by from Good for protein detectionTested in in HEK-293T cells for western blot analysis
Date published: 2018-02-22
Rated 3 out of 5 by from goodIt is good to detect endogenous protein in HeLa cells using western blot analysis.
Date published: 2017-07-17
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ATGL 抗体 (F-7) is rated 4.2 out of 5 by 13.
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