ATG7抗体 (B-9) 推荐用于 mouse, rat 和 human 来源的ATG7 WB, IP, IF 和 ELISA检测

ATG7 抗体 (B-9): sc-376212

ATG7抗体 (B-9) is rated 4.8 out of 5 by 19.
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说明书

    • 抗-ATG7 抗体 B-9 是小鼠单克隆 IgG1 κ,ATG7抗体, 在34篇文献中引用,规格为200 µg/ml
    • 免疫原氨基酸序列1-300位于human物种的ATG7的N-terminus
    • 抗-ATG7 抗体 (B-9) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 ATG7
    • 抗-ATG7 抗体 (B-9) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取ATG7 (B-9): sc-376212免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG1 BP-HRP 是ATG7 Antibody (B-9) for WB首选应用二抗. 这些试剂现在以套装形式提供ATG7 Antibody (B-9) (见下方订购信息).
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We are doing WB to detect Atg7 in Untreated MOLM-13 AML cell line. But we didint get any result yet. Are you suggest anything about that? (5 million cell was used for isolation, 20 ug total protein, 10% gel)

Asked by: Merve
Thank you for your question. Please contact technical service directly for assistance with your experiment.
Answered by: Tech Support Europe
Date published: 2021-04-14

Can APG7 (B-9): sc-376212 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, APG7 (B-9): sc-376212 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works good Used it for western blot, Very clean bands, nice antibody.
Date published: 2018-02-06
Rated 5 out of 5 by from Good Features The antibody is useful for protein imprinting in chicken cardiac muscle cells, and the company's antibody is very good.
Date published: 2017-11-09
Rated 5 out of 5 by from Good results I tested the free sample and I got good results, but only with a more sensitive ECL reagent (Femto, Thermo)
Date published: 2017-10-31
Rated 4 out of 5 by from Worked well I had great results using this antibody at 1:500 dilution.
Date published: 2017-10-23
Rated 5 out of 5 by from Amazing The product quality is nice and western blot result of the band is very clear and strong
Date published: 2017-09-22
Rated 4 out of 5 by from atg7抗体功能评价 抗体挺好用的,没什么杂带,这也是单克隆抗体对比与 多克隆抗体的优点,是做细胞自噬方面相对非常好用且优秀的抗体
Date published: 2017-09-22
Rated 5 out of 5 by from GREAT FEATURES I was so happy to get great results using this antibody to test APG7 levels in T24 and 5637 cells
Date published: 2017-09-08
Rated 4 out of 5 by from Good This antibody worked well in WB with the dilution of 1:500
Date published: 2017-08-25
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