Date published: 2025-9-7
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ATG7 Antibody (B-9) - Western Blotting - Image 378528
ATG7 抗体 (B-9): sc-376212. Caki-1 (A), Jurkat (B), MDA-MB-435S (C), NIH/3T3 (D) 和 HeLa (E) 全细胞裂解液中 ATG7 表达的 Western 印迹分析.
ATG7 抗体 (B-9): sc-376212. Caki-1 (A), Jurkat (B), MDA-MB-435S (C), NIH/3T3 (D) 和 HeLa (E) 全细胞裂解液中 ATG7 表达的 Western 印迹分析.

ATG7 抗体 (B-9): sc-376212

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寫評論提問

说明书
  • ATG7 抗体 B-9 是小鼠单克隆 IgG1 κ，ATG7抗体, 在58篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-300位于human物种的ATG7的N-terminus
  • ATG7 抗体 (B-9) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 ATG7
  • 抗ATG7抗体（B-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ATG7 (B-9): sc-376212的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是ATG7 Antibody (B-9) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与ATG7 Antibody (B-9) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外APG抗体，包括ATG7

ATG7抗体（B-9）是一种IgG1 κ小鼠单克隆ATG7抗体（也称为APG7L抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的ATG7蛋白。ATG7抗体（B-9）既有非偶联型抗ATG7抗体形式，也有多种偶联型抗ATG7抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。在酵母中，自噬是营养饥饿和细胞分化期间生存所必需的过程。自噬过程的特点是将细胞质蛋白非选择性地降解为称为自噬体的膜结构，这依赖于几种蛋白质，包括自噬蛋白ATG5和ATG7。酵母Atg7和人类同源物ATG7与酿酒酵母中的泛素激活酶E1具有相似性，同样负责酶促激活自噬结合系统。Atg5和人类同源物ATG5（也称为凋亡特异性蛋白或APS）作为自噬蛋白APG12的底物发挥作用。这些蛋白质通过共价键结合在一起，形成APG12/ATG5结合物，这是自噬进展所必需的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ATG7 抗体 (B-9) 参考文献:

  1. Apg7p/Cvt2p：一种对自噬至关重要的新型蛋白激活酶  |  Tanida, I., et al. 1999. Mol Biol Cell. 10: 1367-79. PMID: 10233150
  2. m6A mRNA甲基化通过靶向Atg5和Atg7来控制自噬和脂肪生成。  |  Wang, X., et al. 2020. Autophagy. 16: 1221-1235. PMID: 31451060
  3. 基于atg7的细胞自噬激活可逆转阿霉素诱导的心脏毒性。  |  Wang, Y., et al. 2021. Circ Res. 129: e166-e182. PMID: 34384247
  4. METTL3介导的ATG7的m6A修饰调节自噬-GATA4轴，促进细胞衰老和骨关节炎进展。  |  Chen, X., et al. 2022. Ann Rheum Dis. 81: 87-99. PMID: 34706873
  5. 内皮Atg7的消融通过上调Stat1抑制Hif1a表达，从而抑制缺血诱导的血管生成。  |  Yao, H., et al. 2023. Autophagy. 19: 1491-1511. PMID: 36300763
  6. ATG7的去乙酰化驱动巨噬体自噬和LC3相关微自噬的诱导。  |  Xu, Y., et al. 2024. Autophagy. 20: 1134-1146. PMID: 37999993
  7. 细胞质到液泡靶向和自噬利用相同的机制将蛋白质运送到酵母液泡。  |  Scott, SV., et al. 1996. Proc Natl Acad Sci U S A. 93: 12304-8. PMID: 8901576
  8. 参与酵母自噬的基因 APG5 的结构和功能分析。  |  Kametaka, S., et al. 1996. Gene. 178: 139-43. PMID: 8921905
  9. 向酵母液泡的非经典蛋白质分拣  |  Klionsky, DJ. 1998. J Biol Chem. 273: 10807-10. PMID: 9556549
  10. 人类凋亡特异性蛋白与酵母中参与自噬的基因 APG5 的产物之间的同源性。  |  Hammond, EM., et al. 1998. FEBS Lett. 425: 391-5. PMID: 9563500
  11. 自噬所必需的蛋白质连接系统  |  Mizushima, N., et al. 1998. Nature. 395: 395-8. PMID: 9759731
  12. 人类新的蛋白质连接系统。酵母 Apg12p 连接系统的对应物对自噬至关重要。  |  Mizushima, N., et al. 1998. J Biol Chem. 273: 33889-92. PMID: 9852036

订购信息

产品名称产品编号规格价格数量收藏夹

ATG7 抗体 (B-9)

sc-376212
200 µg/ml
$316.00

ATG7 (B-9): m-IgG1 BP-HRP 套装

sc-531715
200 µg Ab; 20 µg BP
$354.00

ATG7 抗体 (B-9) AC

sc-376212 AC
500 µg/ml, 25% agarose
$416.00

ATG7 抗体 (B-9) HRP

sc-376212 HRP
200 µg/ml
$316.00

ATG7 抗体 (B-9) FITC

sc-376212 FITC
200 µg/ml
$330.00

ATG7 抗体 (B-9) PE

sc-376212 PE
200 µg/ml
$343.00

ATG7 抗体 (B-9) Alexa Fluor® 488

sc-376212 AF488
200 µg/ml
$357.00

ATG7 抗体 (B-9) Alexa Fluor® 546

sc-376212 AF546
200 µg/ml
$357.00

ATG7 抗体 (B-9) Alexa Fluor® 594

sc-376212 AF594
200 µg/ml
$357.00

ATG7 抗体 (B-9) Alexa Fluor® 647

sc-376212 AF647
200 µg/ml
$357.00

ATG7 抗体 (B-9) Alexa Fluor® 680

sc-376212 AF680
200 µg/ml
$357.00

ATG7 抗体 (B-9) Alexa Fluor® 790

sc-376212 AF790
200 µg/ml
$357.00

We are doing WB to detect Atg7 in Untreated MOLM-13 AML cell line. But we didint get any result yet. Are you suggest anything about that? (5 million cell was used for isolation, 20 ug total protein, 10% gel)

Asked by: Merve
Thank you for your question. Please contact technical service directly for assistance with your experiment.
Answered by: Tech Support Europe
Date published: 2021-04-14

Can APG7 (B-9): sc-376212 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, APG7 (B-9): sc-376212 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good work!!This antibody gave a strong and clean signal when we used it at a 1:100 diluition with 80μg of protein samples.
Date published: 2022-11-09
Rated 5 out of 5 by from Works goodUsed it for western blot, Very clean bands, nice antibody.
Date published: 2018-02-06
Rated 5 out of 5 by from Good FeaturesThe antibody is useful for protein imprinting in chicken cardiac muscle cells, and the company's antibody is very good.
Date published: 2017-11-09
Rated 5 out of 5 by from Good resultsI tested the free sample and I got good results, but only with a more sensitive ECL reagent (Femto, Thermo)
Date published: 2017-10-31
Rated 4 out of 5 by from Worked wellI had great results using this antibody at 1:500 dilution.
Date published: 2017-10-23
Rated 5 out of 5 by from AmazingThe product quality is nice and western blot result of the band is very clear and strong
Date published: 2017-09-22
Rated 4 out of 5 by from atg7抗体功能评价抗体挺好用的，没什么杂带，这也是单克隆抗体对比与 多克隆抗体的优点，是做细胞自噬方面相对非常好用且优秀的抗体
Date published: 2017-09-22
Rated 5 out of 5 by from GREAT FEATURESI was so happy to get great results using this antibody to test APG7 levels in T24 and 5637 cells
Date published: 2017-09-08
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ATG7 抗体 (B-9) is rated 4.8 out of 5 by 20.
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