Date published: 2025-9-6
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ATF-5 Antibody (E-10) - Western Blotting - Image 281863
ATF-5 抗体 (E-10): sc-377168. 人肝脏组织提取物中 ATF-5 表达的 Western 印迹分析.
ATF-5 抗体 (E-10): sc-377168. 人肝脏组织提取物中 ATF-5 表达的 Western 印迹分析.

ATF-5 抗体 (E-10): sc-377168

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寫評論提問

说明书
  • ATF-5 抗体 E-10 是小鼠单克隆 IgG1 κ，ATF-5抗体, 在8篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-83位于human物种的ATF-5的N-terminus
  • ATF-5 抗体 (E-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 ATF-5
  • 抗ATF-5抗体（E-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-377168 X, 200 µg/0.1 ml)
  • 关于如何获取ATF-5 (E-10): sc-377168的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 ATF-5 抗体 (E-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与ATF-5 抗体 (E-10) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 ATF 抗体包括 ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 α, ATF-5 β 和 ATF-7IP

ATF-5抗体（E-10）是一种IgG1 κ小鼠单克隆ATF-5抗体（也称为ATF5抗体或ATFX抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的ATF-5蛋白。ATF-5抗体（E-10）既有非偶联形式的抗ATF-5抗体，也有多种偶联形式的抗ATF-5抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。真核基因转录受结合模块顺式作用启动子和增强子元件的序列特异性转录因子的调节。ATF/CREB转录因子家族与回文cAMP反应元件（CRE）八核苷酸TGACGTCA结合。这个基因家族中特征最明显的成员包括CREB-1、CREB-2（也称为ATF-4）、CRE-BPa、LZIP（也称为CREB3和Luman）、CREM-1、CREM-2、ATF-1、ATF-2、ATF-3、ATF-5、ATF-6和ATF-7。这类蛋白含有高度分化的N端结构域，但共享一个用于二聚化和DNA结合的C端亮氨酸拉链。ATF-5（ATFx）可以定位到细胞质或细胞核，作为二聚体结合DNA。它与CCND3和PTP4A1相互作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ATF-5 抗体 (E-10) 参考文献:

  1. 人类 Cdc34 和 Rad6B 泛素结合酶靶向环 AMP 诱导转录的抑制因子进行蛋白水解。  |  Pati, D., et al. 1999. Mol Cell Biol. 19: 5001-13. PMID: 10373550
  2. 小鼠 Atf5：两种新型 mRNA 的分子克隆, 基因组组织和气味感觉神经元定域。  |  Hansen, MB., et al. 2002. Genomics. 80: 344-50. PMID: 12213205
  3. DISC1（Disrupted-In-Schizophrenia 1）是一种与中心体相关的蛋白，它与 MAP1A, MIPT3, ATF4/5 和 NUDEL 相互作用：突变时的调控和相互作用的丧失。  |  Morris, JA., et al. 2003. Hum Mol Genet. 12: 1591-608. PMID: 12812986
  4. 携带 LKB1 基因突变的人类肺腺癌的独特基因表达。  |  Fernandez, P., et al. 2004. Oncogene. 23: 5084-91. PMID: 15077168
  5. 激活转录因子 5 的下调是神经祖细胞分化为星形胶质细胞所必需的。  |  Angelastro, JM., et al. 2005. J Neurosci. 25: 3889-99. PMID: 15829641
  6. 神经祖细胞向神经元发展需要 ATF5 的调节表达。  |  Angelastro, JM., et al. 2003. J Neurosci. 23: 4590-600. PMID: 12805299

订购信息

产品名称产品编号规格价格数量收藏夹

ATF-5 抗体 (E-10)

sc-377168
200 µg/ml
$316.00

ATF-5 (E-10): m-IgGκ BP-HRP 套装

sc-523219
200 µg Ab, 40 µg BP
$354.00

ATF-5 抗体 (E-10) X

sc-377168 X
200 µg/0.1 ml
$316.00

ATF-5 抗体 (E-10) AC

sc-377168 AC
500 µg/ml, 25% agarose
$416.00

ATF-5 抗体 (E-10) HRP

sc-377168 HRP
200 µg/ml
$316.00

ATF-5 抗体 (E-10) FITC

sc-377168 FITC
200 µg/ml
$330.00

ATF-5 抗体 (E-10) PE

sc-377168 PE
200 µg/ml
$343.00

ATF-5 抗体 (E-10) Alexa Fluor® 488

sc-377168 AF488
200 µg/ml
$357.00

ATF-5 抗体 (E-10) Alexa Fluor® 546

sc-377168 AF546
200 µg/ml
$357.00

ATF-5 抗体 (E-10) Alexa Fluor® 594

sc-377168 AF594
200 µg/ml
$357.00

ATF-5 抗体 (E-10) Alexa Fluor® 647

sc-377168 AF647
200 µg/ml
$357.00

ATF-5 抗体 (E-10) Alexa Fluor® 680

sc-377168 AF680
200 µg/ml
$357.00

ATF-5 抗体 (E-10) Alexa Fluor® 790

sc-377168 AF790
200 µg/ml
$357.00

Hi, I am interested in purchasing this antibody for an immunofluorescence. However, I feel like there isn't enough information in the caption provided to test it out. Can you direct me to the protocol for this staining? Megan

Asked by: Megan
Thank you for contacting Santa Cruz Biotechnology. https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: BlakeJ
Date published: 2024-06-26

I am using ATF-5 (E-10): sc-377168 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good work!!This antibody gave a strong and clean signal when we used it at a 1:100 diluition with 80μg of protein samples.
Date published: 2022-11-09
Rated 4 out of 5 by from Specific for ATF5 with a close non-specific bandWorks nicely for western blotting of HeLa lysates at a 1:500 dilution at 4 degrees overnight, producing a clean, specific band at approximately 38kDa. However, there is a strong non-specific band that runs very close to the ATF5 band at just above 40kDa.
Date published: 2019-02-07
Rated 4 out of 5 by from Working on Western BlotI got this as a free trial sample. The antibody specifically recognised a band at the right size upon TG treatment in HeLa cells.
Date published: 2018-09-24
Rated 1 out of 5 by from Not specificFound a band of similar size when comparing WT and ATF5KO western blots.
Date published: 2018-03-28
Rated 5 out of 5 by from WB: Single strong band in human liver tissueWB: Single strong band in human liver tissue extract. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Produced excellent immunoperoxidase nuclearProduced excellent immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human uterine cervix tissue. -SCBT QC
Date published: 2015-03-23
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ATF-5抗体 (E-10) is rated 3.8 out of 5 by 6.
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