Date published: 2025-10-25
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ATF-5 Antibody (E-10) - Western Blotting - Image 281863
ATF-5 抗体 (E-10): sc-377168. 人肝脏组织提取物中 ATF-5 表达的 Western 印迹分析.
ATF-5 抗体 (E-10): sc-377168. 人肝脏组织提取物中 ATF-5 表达的 Western 印迹分析.

ATF-5 抗体 (E-10): sc-377168

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说明书
  • ATF-5 抗体 E-10 是小鼠单克隆 IgG2b κ，ATF-5抗体, 在8篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-83位于human物种的ATF-5的N-terminus
  • ATF-5 抗体 (E-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 ATF-5
  • 抗ATF-5抗体（E-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-377168 X, 200 µg/0.1 ml)
  • m-IgGκ BP-HRP是ATF-5 Antibody (E-10) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与ATF-5 Antibody (E-10) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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研究信息

関連項目

附加ATF抗体，包括ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 α, ATF-5 β和ATF-7IP

ATF-5抗体（E-10）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的ATF-5。ATF-5 (E-10) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。ATF-5 在细胞应激反应中起着至关重要的作用，并参与缺氧和营养缺乏等条件下的基因表达调控。ATF-5能够调节转录以应对压力，这对于细胞的存活和适应至关重要，因此ATF-5成为癌症和神经退行性疾病研究的重要目标。ATF-5定位于细胞质和细胞核中，以二聚体的形式与DNA结合，并与其他蛋白质（如参与细胞周期调节和信号转导途径的CCND3和PTP4A1）相互作用。ATF-5所属的ATF/CREB转录因子家族具有保守的C端亮氨酸拉链结构，有利于二聚体形成和DNA结合，而N端结构域则表现出显著的变异性，从而实现多种调节功能。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ATF-5 抗体 (E-10) 参考文献:

  1. 人类 Cdc34 和 Rad6B 泛素结合酶靶向环 AMP 诱导转录的抑制因子进行蛋白水解。  |  Pati, D., et al. 1999. Mol Cell Biol. 19: 5001-13. PMID: 10373550
  2. 小鼠 Atf5：两种新型 mRNA 的分子克隆, 基因组组织和气味感觉神经元定域。  |  Hansen, MB., et al. 2002. Genomics. 80: 344-50. PMID: 12213205
  3. DISC1（Disrupted-In-Schizophrenia 1）是一种与中心体相关的蛋白，它与 MAP1A, MIPT3, ATF4/5 和 NUDEL 相互作用：突变时的调控和相互作用的丧失。  |  Morris, JA., et al. 2003. Hum Mol Genet. 12: 1591-608. PMID: 12812986
  4. 携带 LKB1 基因突变的人类肺腺癌的独特基因表达。  |  Fernandez, P., et al. 2004. Oncogene. 23: 5084-91. PMID: 15077168
  5. 激活转录因子 5 的下调是神经祖细胞分化为星形胶质细胞所必需的。  |  Angelastro, JM., et al. 2005. J Neurosci. 25: 3889-99. PMID: 15829641
  6. 神经祖细胞向神经元发展需要 ATF5 的调节表达。  |  Angelastro, JM., et al. 2003. J Neurosci. 23: 4590-600. PMID: 12805299

订购信息

产品名称产品编号规格价格数量收藏夹

ATF-5 抗体 (E-10)

sc-377168
200 µg/ml
$316.00

ATF-5 (E-10): m-IgGκ BP-HRP 套装

sc-523219
200 µg Ab, 40 µg BP
$354.00

ATF-5 抗体 (E-10) X

sc-377168 X
200 µg/0.1 ml
$316.00

ATF-5 抗体 (E-10) AC

sc-377168 AC
500 µg/ml, 25% agarose
$416.00

ATF-5 抗体 (E-10) HRP

sc-377168 HRP
200 µg/ml
$316.00

ATF-5 抗体 (E-10) FITC

sc-377168 FITC
200 µg/ml
$330.00

ATF-5 抗体 (E-10) PE

sc-377168 PE
200 µg/ml
$343.00

ATF-5 抗体 (E-10) Alexa Fluor® 488

sc-377168 AF488
200 µg/ml
$357.00

ATF-5 抗体 (E-10) Alexa Fluor® 546

sc-377168 AF546
200 µg/ml
$357.00

ATF-5 抗体 (E-10) Alexa Fluor® 594

sc-377168 AF594
200 µg/ml
$357.00

ATF-5 抗体 (E-10) Alexa Fluor® 647

sc-377168 AF647
200 µg/ml
$357.00

ATF-5 抗体 (E-10) Alexa Fluor® 680

sc-377168 AF680
200 µg/ml
$357.00

ATF-5 抗体 (E-10) Alexa Fluor® 790

sc-377168 AF790
200 µg/ml
$357.00

Hi, I am interested in purchasing this antibody for an immunofluorescence. However, I feel like there isn't enough information in the caption provided to test it out. Can you direct me to the protocol for this staining? Megan

Asked by: Megan
Thank you for contacting Santa Cruz Biotechnology. https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: BlakeJ
Date published: 2024-06-26

I am using ATF-5 (E-10): sc-377168 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good work!!This antibody gave a strong and clean signal when we used it at a 1:100 diluition with 80μg of protein samples.
Date published: 2022-11-09
Rated 4 out of 5 by from Specific for ATF5 with a close non-specific bandWorks nicely for western blotting of HeLa lysates at a 1:500 dilution at 4 degrees overnight, producing a clean, specific band at approximately 38kDa. However, there is a strong non-specific band that runs very close to the ATF5 band at just above 40kDa.
Date published: 2019-02-07
Rated 4 out of 5 by from Working on Western BlotI got this as a free trial sample. The antibody specifically recognised a band at the right size upon TG treatment in HeLa cells.
Date published: 2018-09-24
Rated 1 out of 5 by from Not specificFound a band of similar size when comparing WT and ATF5KO western blots.
Date published: 2018-03-28
Rated 5 out of 5 by from WB: Single strong band in human liver tissueWB: Single strong band in human liver tissue extract. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Produced excellent immunoperoxidase nuclearProduced excellent immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human uterine cervix tissue. -SCBT QC
Date published: 2015-03-23
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ATF-5抗体 (E-10) is rated 3.8 out of 5 by 6.
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